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Title: Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification

Patent ·
OSTI ID:1327928
; ;

Provided herein are methods and systems for assembling, solubilizing and/or purifying a membrane associated protein in a nanolipoprotein particle, which comprise a temperature transition cycle performed in presence of a detergent, wherein during the temperature transition cycle the nanolipoprotein components are brought to a temperature above and below the gel to liquid crystalling transition temperature of the membrane forming lipid of the nanolipoprotein particle.

Research Organization:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC52-07NA27344
Assignee:
Lawrence Livermore National Security, LLC (Livermore, CA)
Patent Number(s):
9,458,191
Application Number:
14/536,513
OSTI ID:
1327928
Resource Relation:
Patent File Date: 2014 Nov 07
Country of Publication:
United States
Language:
English

References (16)

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Nanoparticle-based vaccine delivery system containing adjuvant patent-application May 2008
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Copper-catalysed ligation of azides and acetylenes patent May 2008
Nanolipoprotein Particles and Related Compositions, Methods and Systems patent-application December 2009
Photocatalytic Hydrogen Production And Polypeptides Capable Of Same patent-application August 2010
Membrane scaffold proteins and tethered membrane proteins patent-application September 2006
Photothermal detection of nucleic acid hybridization patent-application September 2004
Nanoparticle-Based vaccine delivery system containing adjuvant patent-application August 2006

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