Application of Long Sequence Reads To Improve Genomes for Clostridium thermocellum AD2, Clostridium thermocellum LQRI, and Pelosinus fermentans R7

Utturkar, Sagar M.; Bayer, Edward A.; Borovok, Ilya; Lamed, Raphael; Hurt, Richard A.; Land, Miriam L.; Klingeman, Dawn M.; Elias, Dwayne; Zhou, Jizhong; Huntemann, Marcel; Clum, Alicia; Pillay, Manoj; Palaniappan, Krishnaveni; Varghese, Neha; Mikhailova, Natalia; Stamatis, Dimitrios; Reddy, T. B. K.; Ngan, Chew Yee; Daum, Chris; Shapiro, Nicole; Markowitz, Victor; Ivanova, Natalia; Kyrpides, Nikos; Woyke, Tanja; Brown, Steven D.

doi:10.1128/genomeA.01043-16

Title: Application of Long Sequence Reads To Improve Genomes for Clostridium thermocellum AD2, Clostridium thermocellum LQRI, and Pelosinus fermentans R7

Journal Article · Thu Oct 27 00:00:00 EDT 2016 · Genome Announcements

DOI:https://doi.org/10.1128/genomeA.01043-16· OSTI ID:1786439

Utturkar, Sagar M. ^[1]; Bayer, Edward A. ^[2]; Borovok, Ilya ^[3]; Lamed, Raphael ^[3]; Hurt, Richard A. ^[4]; Land, Miriam L. ^[4]; Klingeman, Dawn M. ^[5];

^[4]; Zhou, Jizhong ^[6]; Huntemann, Marcel ^[7]; Clum, Alicia ^[7]; Pillay, Manoj ^[7]; Palaniappan, Krishnaveni ^[7]; Varghese, Neha ^[7]; Mikhailova, Natalia ^[7]; Stamatis, Dimitrios ^[7]; Reddy, T. B. K. ^[7]; Ngan, Chew Yee ^[7]; Daum, Chris ^[7]; Shapiro, Nicole ^[7] more »

Graduate School of Genome Science and Technology, University of Tennessee, Knoxville, Tennessee, USA
Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot, Israel
Department of Molecular Microbiology and Biotechnology, Tel-Aviv University, Ramat Aviv, Israel
Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA
Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA, BioEnergy Science Center, Oak Ridge, Tennessee, USA
Institute for Environmental Genomics, University of Oklahoma, Norman, Oklahoma, USA
DOE Joint Genome Institute, Walnut Creek, California, USA
Graduate School of Genome Science and Technology, University of Tennessee, Knoxville, Tennessee, USA, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA, BioEnergy Science Center, Oak Ridge, Tennessee, USA

We and others have shown the utility of long sequence reads to improve genome assembly quality. In this study, we generated PacBio DNA sequence data to improve the assemblies of draft genomes forClostridium thermocellumAD2,Clostridium thermocellumLQRI, andPelosinus fermentansR7.

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Research Organization:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-00OR22725; AC02-05CH11231

OSTI ID:: 1786439

Alternate ID(s):: OSTI ID: 1327678; OSTI ID: 1378997

Journal Information:: Genome Announcements, Journal Name: Genome Announcements Vol. 4 Journal Issue: 5; ISSN 2169-8287

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

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