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Title: A multi-omic future for microbiome studies

Abstract

Microbes constitute about a third of the Earth’s biomass and play critical roles in sustaining life. While results from multiple sequence-based studies have illustrated the importance of microbial communities for human health and the environment, additional technological developments are still needed to gain more insight into their functions [1]. To date, the majority of sequencing studies have focused on the 16S rRNA gene as a phylogenetic marker. This approach has enabled exploration of microbial compositions in a range of sample types, while bypassing the need for cultivation. 16S rRNA gene sequencing has also enabled a vast majority of microorganisms never previously isolated in culture to be identified and placed into a phylogenetic context [2]. These technologies have been utilized to map the locations of microbes inhabiting various locations of the body [3]. Similarly, sequencing has been used to determine the identities and distributions of microorganisms inhabiting different ecosystems [4, 5], and efforts in single cell sequencing of the microbiome have helped fill in missing branches of the phylogenetic tree [6].

Authors:
;
Publication Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org.:
USDOE
OSTI Identifier:
1327110
Report Number(s):
PNNL-SA-116189
Journal ID: ISSN 2058-5276; 48680; 453040220
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Nature Microbiology
Additional Journal Information:
Journal Volume: 1; Journal Issue: 5; Journal ID: ISSN 2058-5276
Country of Publication:
United States
Language:
English
Subject:
Environmental Molecular Sciences Laboratory

Citation Formats

Jansson, Janet K., and Baker, Erin S. A multi-omic future for microbiome studies. United States: N. p., 2016. Web. doi:10.1038/NMICROBIOL.2016.49.
Jansson, Janet K., & Baker, Erin S. A multi-omic future for microbiome studies. United States. doi:10.1038/NMICROBIOL.2016.49.
Jansson, Janet K., and Baker, Erin S. Tue . "A multi-omic future for microbiome studies". United States. doi:10.1038/NMICROBIOL.2016.49.
@article{osti_1327110,
title = {A multi-omic future for microbiome studies},
author = {Jansson, Janet K. and Baker, Erin S.},
abstractNote = {Microbes constitute about a third of the Earth’s biomass and play critical roles in sustaining life. While results from multiple sequence-based studies have illustrated the importance of microbial communities for human health and the environment, additional technological developments are still needed to gain more insight into their functions [1]. To date, the majority of sequencing studies have focused on the 16S rRNA gene as a phylogenetic marker. This approach has enabled exploration of microbial compositions in a range of sample types, while bypassing the need for cultivation. 16S rRNA gene sequencing has also enabled a vast majority of microorganisms never previously isolated in culture to be identified and placed into a phylogenetic context [2]. These technologies have been utilized to map the locations of microbes inhabiting various locations of the body [3]. Similarly, sequencing has been used to determine the identities and distributions of microorganisms inhabiting different ecosystems [4, 5], and efforts in single cell sequencing of the microbiome have helped fill in missing branches of the phylogenetic tree [6].},
doi = {10.1038/NMICROBIOL.2016.49},
journal = {Nature Microbiology},
issn = {2058-5276},
number = 5,
volume = 1,
place = {United States},
year = {2016},
month = {4}
}