skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Chemical imaging of molecular changes in a hydrated single cell by dynamic secondary ion mass spectrometry and super-resolution microscopy

Abstract

Chemical imaging of single cells is important in capturing biological dynamics. Single cell correlative imaging is realized between structured illumination microscopy (SIM) and time-of-flight secondary ion mass spectrometry (ToF-SIMS) using System for Analysis at the Liquid Vacuum Interface (SALVI), a multimodal microreactor. SIM characterized cells and guided subsequent ToF-SIMS analysis. Dynamic ToF-SIMS provided time- and space-resolved cell molecular mapping. Lipid fragments were identified in the hydrated cell membrane. Principal component analysis was used to elucidate chemical component differences among mouse lung cells that uptake zinc oxide nanoparticles. Our results provided submicron chemical spatial mapping for investigations of cell dynamics at the molecular level.

Authors:
; ; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org.:
USDOE
OSTI Identifier:
1327108
Report Number(s):
PNNL-SA-110602
Journal ID: ISSN 1757-9694; 49143; 48143; 48672; 48671
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Integrative Biology
Additional Journal Information:
Journal Volume: 8; Journal Issue: 5; Journal ID: ISSN 1757-9694
Publisher:
Royal Society of Chemistry
Country of Publication:
United States
Language:
English
Subject:
correlative chemical imaging • microfluidic • surface analysis • nanoparticle toxicity • principal component analysis; Environmental Molecular Sciences Laboratory

Citation Formats

Hua, Xin, Szymanski, Craig, Wang, Zhaoying, Zhou, Yufan, Ma, Xiang, Yu, Jiachao, Evans, James, Orr, Galya, Liu, Songqin, Zhu, Zihua, and Yu, Xiao-Ying. Chemical imaging of molecular changes in a hydrated single cell by dynamic secondary ion mass spectrometry and super-resolution microscopy. United States: N. p., 2016. Web. doi:10.1039/c5ib00308c.
Hua, Xin, Szymanski, Craig, Wang, Zhaoying, Zhou, Yufan, Ma, Xiang, Yu, Jiachao, Evans, James, Orr, Galya, Liu, Songqin, Zhu, Zihua, & Yu, Xiao-Ying. Chemical imaging of molecular changes in a hydrated single cell by dynamic secondary ion mass spectrometry and super-resolution microscopy. United States. doi:10.1039/c5ib00308c.
Hua, Xin, Szymanski, Craig, Wang, Zhaoying, Zhou, Yufan, Ma, Xiang, Yu, Jiachao, Evans, James, Orr, Galya, Liu, Songqin, Zhu, Zihua, and Yu, Xiao-Ying. Fri . "Chemical imaging of molecular changes in a hydrated single cell by dynamic secondary ion mass spectrometry and super-resolution microscopy". United States. doi:10.1039/c5ib00308c.
@article{osti_1327108,
title = {Chemical imaging of molecular changes in a hydrated single cell by dynamic secondary ion mass spectrometry and super-resolution microscopy},
author = {Hua, Xin and Szymanski, Craig and Wang, Zhaoying and Zhou, Yufan and Ma, Xiang and Yu, Jiachao and Evans, James and Orr, Galya and Liu, Songqin and Zhu, Zihua and Yu, Xiao-Ying},
abstractNote = {Chemical imaging of single cells is important in capturing biological dynamics. Single cell correlative imaging is realized between structured illumination microscopy (SIM) and time-of-flight secondary ion mass spectrometry (ToF-SIMS) using System for Analysis at the Liquid Vacuum Interface (SALVI), a multimodal microreactor. SIM characterized cells and guided subsequent ToF-SIMS analysis. Dynamic ToF-SIMS provided time- and space-resolved cell molecular mapping. Lipid fragments were identified in the hydrated cell membrane. Principal component analysis was used to elucidate chemical component differences among mouse lung cells that uptake zinc oxide nanoparticles. Our results provided submicron chemical spatial mapping for investigations of cell dynamics at the molecular level.},
doi = {10.1039/c5ib00308c},
journal = {Integrative Biology},
issn = {1757-9694},
number = 5,
volume = 8,
place = {United States},
year = {2016},
month = {1}
}

Works referenced in this record:

Putting Molecules in Their Place: PUTTING MOLECULES IN THEIR PLACE
journal, December 2013

  • Cinquin, Bertrand P.; Do, Myan; McDermott, Gerry
  • Journal of Cellular Biochemistry, Vol. 115, Issue 2
  • DOI: 10.1002/jcb.24658

Nanoscale NMR spectroscopy and imaging of multiple nuclear species
journal, January 2015

  • DeVience, Stephen J.; Pham, Linh M.; Lovchinsky, Igor
  • Nature Nanotechnology, Vol. 10, Issue 2
  • DOI: 10.1038/nnano.2014.313

In Situ Characterization of Hydrated Proteins in Water by SALVI and ToF-SIMS
journal, January 2016

  • Yu, Jiachao; Zhou, Yufan; Hua, Xin
  • Journal of Visualized Experiments, Issue 108
  • DOI: 10.3791/53708

Correlated imaging – a grand challenge in chemical analysis
journal, January 2013

  • Masyuko, Rachel; Lanni, Eric J.; Sweedler, Jonathan V.
  • The Analyst, Vol. 138, Issue 7
  • DOI: 10.1039/c3an36416j

Two-dimensional and three-dimensional dynamic imaging of live biofilms in a microchannel by time-of-flight secondary ion mass spectrometry
journal, May 2015

  • Hua, Xin; Marshall, Matthew J.; Xiong, Yijia
  • Biomicrofluidics, Vol. 9, Issue 3
  • DOI: 10.1063/1.4919807

Making a hybrid microfluidic platform compatible for in situ imaging by vacuum-based techniques
journal, November 2011

  • Yang, Li; Yu, Xiao-Ying; Zhu, Zihua
  • Journal of Vacuum Science & Technology A: Vacuum, Surfaces, and Films, Vol. 29, Issue 6
  • DOI: 10.1116/1.3654147

Zinc Oxide—From Synthesis to Application: A Review
journal, April 2014

  • Kołodziejczak-Radzimska, Agnieszka; Jesionowski, Teofil
  • Materials, Vol. 7, Issue 4
  • DOI: 10.3390/ma7042833

Droplet Microfluidics-A Tool for Single-Cell Analysis
journal, November 2012

  • Joensson, Haakan N.; Andersson Svahn, Helene
  • Angewandte Chemie International Edition, Vol. 51, Issue 49
  • DOI: 10.1002/anie.201200460

In situ SEM and ToF-SIMS analysis of IgG conjugated gold nanoparticles at aqueous surfaces : In situ SEM and ToF-SIMS analysis of aqueous Au nanoparticles
journal, March 2013

  • Yang, Li; Zhu, Zihua; Yu, Xiao-Ying
  • Surface and Interface Analysis, Vol. 46, Issue 4
  • DOI: 10.1002/sia.5252

Graphene-enabled electron microscopy and correlated super-resolution microscopy of wet cells
journal, June 2015

  • Wojcik, Michal; Hauser, Margaret; Li, Wan
  • Nature Communications, Vol. 6, Issue 1
  • DOI: 10.1038/ncomms8384

Membrane lipids: where they are and how they behave
journal, February 2008

  • van Meer, Gerrit; Voelker, Dennis R.; Feigenson, Gerald W.
  • Nature Reviews Molecular Cell Biology, Vol. 9, Issue 2
  • DOI: 10.1038/nrm2330

Aerosolized ZnO Nanoparticles Induce Toxicity in Alveolar Type II Epithelial Cells at the Air-Liquid Interface
journal, September 2011

  • Xie, Yumei; Williams, Nolann G.; Tolic, Ana
  • Toxicological Sciences, Vol. 125, Issue 2
  • DOI: 10.1093/toxsci/kfr251

TOF-SIMS: Accurate mass scale calibration
journal, April 2006

  • Green, F. M.; Gilmore, I. S.; Seah, M. P.
  • Journal of the American Society for Mass Spectrometry, Vol. 17, Issue 4
  • DOI: 10.1016/j.jasms.2005.12.005

In situ thermo-TOF-SIMS study of thermal decomposition of zinc acetate dihydrate
journal, January 2004

  • Ghule, Anil Vithal; Ghule, Kalyani; Chen, Chin-Yuan
  • Journal of Mass Spectrometry, Vol. 39, Issue 10
  • DOI: 10.1002/jms.721

Imaging Hydrated Microbial Extracellular Polymers: Comparative Analysis by Electron Microscopy
journal, December 2010

  • Dohnalkova, Alice C.; Marshall, Matthew J.; Arey, Bruce W.
  • Applied and Environmental Microbiology, Vol. 77, Issue 4
  • DOI: 10.1128/AEM.02001-10