skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Use and engineering of efflux pumps for the export of olefins in microbes

Abstract

The scope of the project is to investigate efflux pump systems in engineered host microorganisms, such as E. coli, and develop a pump engineered to export a target compound. To initiate the project in coordination with other TOTAL driven projects, the first target compound to be studied was 1-hexene. However, we were investigating other chemicals as Styrene. The main goal of the project was to generate a set of optimized efflux pump systems for microorganisms (E. coli and Streptomyces or other host) engineered to contain biosynthetic pathways to export large titers of target compounds that are toxic (or accumulate and push back biosynthesis) to the host cell. An optimized microbial host will utilize specific and efficient cell wall located pumps to extrude harmful target compounds and enable greater production of these compounds.

Authors:
 [1]
  1. Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1326827
Report Number(s):
LBNL-1005835
ir:1005835
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Mukhopadhyay, Aindrila. Use and engineering of efflux pumps for the export of olefins in microbes. United States: N. p., 2016. Web. doi:10.2172/1326827.
Mukhopadhyay, Aindrila. Use and engineering of efflux pumps for the export of olefins in microbes. United States. doi:10.2172/1326827.
Mukhopadhyay, Aindrila. Thu . "Use and engineering of efflux pumps for the export of olefins in microbes". United States. doi:10.2172/1326827. https://www.osti.gov/servlets/purl/1326827.
@article{osti_1326827,
title = {Use and engineering of efflux pumps for the export of olefins in microbes},
author = {Mukhopadhyay, Aindrila},
abstractNote = {The scope of the project is to investigate efflux pump systems in engineered host microorganisms, such as E. coli, and develop a pump engineered to export a target compound. To initiate the project in coordination with other TOTAL driven projects, the first target compound to be studied was 1-hexene. However, we were investigating other chemicals as Styrene. The main goal of the project was to generate a set of optimized efflux pump systems for microorganisms (E. coli and Streptomyces or other host) engineered to contain biosynthetic pathways to export large titers of target compounds that are toxic (or accumulate and push back biosynthesis) to the host cell. An optimized microbial host will utilize specific and efficient cell wall located pumps to extrude harmful target compounds and enable greater production of these compounds.},
doi = {10.2172/1326827},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Thu Jul 14 00:00:00 EDT 2016},
month = {Thu Jul 14 00:00:00 EDT 2016}
}

Technical Report:

Save / Share: