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Title: Systems biology-guided biodesign of consolidated lignin conversion

Abstract

Lignin is the second most abundant biopolymer on the earth, yet its utilization for fungible products is complicated by its recalcitrant nature and remains a major challenge for sustainable lignocellulosic biorefineries. In this study, we used a systems biology approach to reveal the carbon utilization pattern and lignin degradation mechanisms in a unique lignin-utilizing Pseudomonas putida strain (A514). The mechanistic study further guided the design of three functional modules to enable a consolidated lignin bioconversion route. First, P. putida A514 mobilized a dye peroxidase-based enzymatic system for lignin depolymerization. This system could be enhanced by overexpressing a secreted multifunctional dye peroxidase to promote a two-fold enhancement of cell growth on insoluble kraft lignin. Second, A514 employed a variety of peripheral and central catabolism pathways to metabolize aromatic compounds, which can be optimized by overexpressing key enzymes. Third, the β-oxidation of fatty acid was up-regulated, whereas fatty acid synthesis was down-regulated when A514 was grown on lignin and vanillic acid. Therefore, the functional module for polyhydroxyalkanoate (PHA) production was designed to rechannel β-oxidation products. As a result, PHA content reached 73% per cell dry weight (CDW). Further integrating the three functional modules enhanced the production of PHA from kraft lignin andmore » biorefinery waste. Furthermore, this study elucidated lignin conversion mechanisms in bacteria with potential industrial implications and laid out the concept for engineering a consolidated lignin conversion route.« less

Authors:
 [1];  [2];  [3];  [2];  [2];  [4];  [2];  [2];  [4];  [2];  [5];  [2]
  1. Texas A & M Univ., College Station, TX (United States); Zhejiang Univ., Hangzhou (China)
  2. Texas A & M Univ., College Station, TX (United States)
  3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  4. Michigan State Univ., East Lansing, MI (United States)
  5. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1326560
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Green Chemistry
Additional Journal Information:
Journal Name: Green Chemistry; Journal ID: ISSN 1463-9262
Publisher:
Royal Society of Chemistry
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Lin, Lu, Cheng, Yanbing, Pu, Yunqiao, Sun, Su, Li, Xiao, Jin, Mingjie, Pierson, Elizabeth A., Gross, Dennis C., Dale, Bruce E., Dai, Susie Y., Ragauskas, Arthur J., and Yuan, Joshua S.. Systems biology-guided biodesign of consolidated lignin conversion. United States: N. p., 2016. Web. doi:10.1039/C6GC01131D.
Lin, Lu, Cheng, Yanbing, Pu, Yunqiao, Sun, Su, Li, Xiao, Jin, Mingjie, Pierson, Elizabeth A., Gross, Dennis C., Dale, Bruce E., Dai, Susie Y., Ragauskas, Arthur J., & Yuan, Joshua S.. Systems biology-guided biodesign of consolidated lignin conversion. United States. doi:10.1039/C6GC01131D.
Lin, Lu, Cheng, Yanbing, Pu, Yunqiao, Sun, Su, Li, Xiao, Jin, Mingjie, Pierson, Elizabeth A., Gross, Dennis C., Dale, Bruce E., Dai, Susie Y., Ragauskas, Arthur J., and Yuan, Joshua S.. Tue . "Systems biology-guided biodesign of consolidated lignin conversion". United States. doi:10.1039/C6GC01131D. https://www.osti.gov/servlets/purl/1326560.
@article{osti_1326560,
title = {Systems biology-guided biodesign of consolidated lignin conversion},
author = {Lin, Lu and Cheng, Yanbing and Pu, Yunqiao and Sun, Su and Li, Xiao and Jin, Mingjie and Pierson, Elizabeth A. and Gross, Dennis C. and Dale, Bruce E. and Dai, Susie Y. and Ragauskas, Arthur J. and Yuan, Joshua S.},
abstractNote = {Lignin is the second most abundant biopolymer on the earth, yet its utilization for fungible products is complicated by its recalcitrant nature and remains a major challenge for sustainable lignocellulosic biorefineries. In this study, we used a systems biology approach to reveal the carbon utilization pattern and lignin degradation mechanisms in a unique lignin-utilizing Pseudomonas putida strain (A514). The mechanistic study further guided the design of three functional modules to enable a consolidated lignin bioconversion route. First, P. putida A514 mobilized a dye peroxidase-based enzymatic system for lignin depolymerization. This system could be enhanced by overexpressing a secreted multifunctional dye peroxidase to promote a two-fold enhancement of cell growth on insoluble kraft lignin. Second, A514 employed a variety of peripheral and central catabolism pathways to metabolize aromatic compounds, which can be optimized by overexpressing key enzymes. Third, the β-oxidation of fatty acid was up-regulated, whereas fatty acid synthesis was down-regulated when A514 was grown on lignin and vanillic acid. Therefore, the functional module for polyhydroxyalkanoate (PHA) production was designed to rechannel β-oxidation products. As a result, PHA content reached 73% per cell dry weight (CDW). Further integrating the three functional modules enhanced the production of PHA from kraft lignin and biorefinery waste. Furthermore, this study elucidated lignin conversion mechanisms in bacteria with potential industrial implications and laid out the concept for engineering a consolidated lignin conversion route.},
doi = {10.1039/C6GC01131D},
journal = {Green Chemistry},
number = ,
volume = ,
place = {United States},
year = {Tue Jul 12 00:00:00 EDT 2016},
month = {Tue Jul 12 00:00:00 EDT 2016}
}

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