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Title: Determining the syringyl/guaiacyl lignin ratio in the vessel and fiber cell walls of transgenic Populus plants

Abstract

Observation of the spatial lignin distribution throughout the plant cell wall provides insight into the physicochemical characteristics of lignocellulosic biomass. The distribution of syringyl (S) and guaiacyl (G) lignin in cell walls of a genetically modified Populus deltoides and its corresponding empty vector control were analyzed with time-of-flight secondary ion mass spectrometry (ToF-SIMS) and then mapped to determine the S/G lignin ratio of the sample surface and specific regions of interest (ROIs). The surface characterizations of transgenic cross-sections within 1 cm vertical distance of each other on the stem possess similar S/G lignin ratios. Furthermore, the analysis of the ROIs determined that there was a 50% decrease in the S/G lignin ratio of the transgenic xylem fiber cell walls.

Authors:
 [1];  [1];  [1];  [1]
  1. Georgia Inst. of Technology, Atlanta, GA (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1326559
Grant/Contract Number:
AC05-00OR22725
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Energy and Fuels
Additional Journal Information:
Journal Volume: 30; Journal Issue: 7; Journal ID: ISSN 0887-0624
Publisher:
American Chemical Society (ACS)
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS

Citation Formats

Tolbert, Allison K., Ma, Tao, Kalluri, Udaya C., and Ragauskas, Arthur J. Determining the syringyl/guaiacyl lignin ratio in the vessel and fiber cell walls of transgenic Populus plants. United States: N. p., 2016. Web. doi:10.1021/acs.energyfuels.6b00560.
Tolbert, Allison K., Ma, Tao, Kalluri, Udaya C., & Ragauskas, Arthur J. Determining the syringyl/guaiacyl lignin ratio in the vessel and fiber cell walls of transgenic Populus plants. United States. doi:10.1021/acs.energyfuels.6b00560.
Tolbert, Allison K., Ma, Tao, Kalluri, Udaya C., and Ragauskas, Arthur J. Mon . "Determining the syringyl/guaiacyl lignin ratio in the vessel and fiber cell walls of transgenic Populus plants". United States. doi:10.1021/acs.energyfuels.6b00560. https://www.osti.gov/servlets/purl/1326559.
@article{osti_1326559,
title = {Determining the syringyl/guaiacyl lignin ratio in the vessel and fiber cell walls of transgenic Populus plants},
author = {Tolbert, Allison K. and Ma, Tao and Kalluri, Udaya C. and Ragauskas, Arthur J.},
abstractNote = {Observation of the spatial lignin distribution throughout the plant cell wall provides insight into the physicochemical characteristics of lignocellulosic biomass. The distribution of syringyl (S) and guaiacyl (G) lignin in cell walls of a genetically modified Populus deltoides and its corresponding empty vector control were analyzed with time-of-flight secondary ion mass spectrometry (ToF-SIMS) and then mapped to determine the S/G lignin ratio of the sample surface and specific regions of interest (ROIs). The surface characterizations of transgenic cross-sections within 1 cm vertical distance of each other on the stem possess similar S/G lignin ratios. Furthermore, the analysis of the ROIs determined that there was a 50% decrease in the S/G lignin ratio of the transgenic xylem fiber cell walls.},
doi = {10.1021/acs.energyfuels.6b00560},
journal = {Energy and Fuels},
number = 7,
volume = 30,
place = {United States},
year = {Mon Jun 20 00:00:00 EDT 2016},
month = {Mon Jun 20 00:00:00 EDT 2016}
}

Journal Article:
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Cited by: 1work
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