Cell wall targeted in planta iron accumulation enhances biomass conversion and seed iron concentration in Arabidopsis and rice

Yang, Haibing; Wei, Hui; Ma, Guojie; Antunes, Mauricio S.; Vogt, Stefan; Cox, Joseph; Zhang, Xiao; Liu, Xiping; Bu, Lintao; Gleber, S. Charlotte; Carpita, Nicholas C.; Makowski, Lee; Himmel, Michael E.; Tucker, Melvin P.; McCann, Maureen C.; Murphy, Angus S.; Peer, Wendy A.

doi:10.1111/pbi.12557

Title: Cell wall targeted in planta iron accumulation enhances biomass conversion and seed iron concentration in Arabidopsis and rice

Journal Article · Thu Apr 07 00:00:00 EDT 2016 · Plant Biotechnology Journal

DOI:https://doi.org/10.1111/pbi.12557· OSTI ID:1324324

Yang, Haibing ^[1]; Wei, Hui ^[2]; Ma, Guojie ^[3]; Antunes, Mauricio S. ^[4]; Vogt, Stefan ^[5]; Cox, Joseph ^[3]; Zhang, Xiao ^[6]; Liu, Xiping ^[3]; Bu, Lintao ^[7]; Gleber, S. Charlotte ^[5]; Carpita, Nicholas C. ^[8]; Makowski, Lee ^[9]; Himmel, Michael E. ^[2]; Tucker, Melvin P. ^[5]; McCann, Maureen C. ^[4]; Murphy, Angus S. ^[10]; Peer, Wendy A. ^[11]

Center for Direct Catalytic Conversion Of Biomass to Biofuels (C3Bio) Purdue University West Lafayette IN USA, Department of Horticulture Purdue University West Lafayette IN USA, Department of Biological Sciences Purdue University West Lafayette IN USA
Biosciences Center National Renewable Energy Laboratory Golden CO USA
Center for Direct Catalytic Conversion Of Biomass to Biofuels (C3Bio) Purdue University West Lafayette IN USA, Department of Horticulture Purdue University West Lafayette IN USA
Center for Direct Catalytic Conversion Of Biomass to Biofuels (C3Bio) Purdue University West Lafayette IN USA, Department of Biological Sciences Purdue University West Lafayette IN USA
X‐ray Science Division Advanced Photon Source Argonne National Laboratory Argonne IL USA
Department of Horticulture Purdue University West Lafayette IN USA
National Bioenergy Center National Renewable Energy Laboratory Golden CO USA
Department of Biological Sciences Purdue University West Lafayette IN USA, Department of Botany and Plant Pathology Purdue University West Lafayette IN USA
Department of Bioengineering Northeastern University Boston MA USA, Department of Chemistry and Chemical Biology Northeastern University Boston MA USA
Center for Direct Catalytic Conversion Of Biomass to Biofuels (C3Bio) Purdue University West Lafayette IN USA, Department of Horticulture Purdue University West Lafayette IN USA, Department of Plant Science and Landscape Architecture University of Maryland College Park MD USA
Center for Direct Catalytic Conversion Of Biomass to Biofuels (C3Bio) Purdue University West Lafayette IN USA, Department of Horticulture Purdue University West Lafayette IN USA, Department of Plant Science and Landscape Architecture University of Maryland College Park MD USA, Department of Environmental Science and Technology University of Maryland College Park MD USA

Conversion of nongrain biomass into liquid fuel is a sustainable approach to energy demands as global population increases. Previously, we showed that iron can act as a catalyst to enhance the degradation of lignocellulosic biomass for biofuel production. However, direct addition of iron catalysts to biomass pretreatment is diffusion-limited, would increase the cost and complexity of biorefinery unit operations and may have deleterious environmental impacts. Here, we show a new strategy for in planta accumulation of iron throughout the volume of the cell wall where iron acts as a catalyst in the deconstruction of lignocellulosic biomass. We engineered CBM-IBP fusion polypeptides composed of a carbohydrate-binding module family 11 (CBM11) and an iron-binding peptide (IBP) for secretion into Arabidopsis and rice cell walls. CBM-IBP transformed Arabidopsis and rice plants show significant increases in iron accumulation and biomass conversion compared to respective controls. Further, CBM-IBP rice shows a 35% increase in seed iron concentration and a 40% increase in seed yield in greenhouse experiments. In conclusion, CBM-IBP rice potentially could be used to address iron deficiency, the most common and widespread nutritional disorder according to the World Health Organization.

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Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States); Energy Frontier Research Centers (EFRC) (United States). Center for Direct Catalytic Conversion of Biomass to Biofuels (C3Bio)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: DE‐SC0000997; DE‐AC02‐06CH11357; AC36-08GO28308; SC000997

OSTI ID:: 1324324

Alternate ID(s):: OSTI ID: 1324325; OSTI ID: 1325506

Report Number(s):: NREL/JA-2700-66087

Journal Information:: Plant Biotechnology Journal, Journal Name: Plant Biotechnology Journal Vol. 14 Journal Issue: 10; ISSN 1467-7644

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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Cited by: 13 works

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