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Title: Feruloyl-CoA:monolignol transferase

Abstract

The invention relates to nucleic acids encoding a feruloyl-CoA:monolignol transferase and the feruloyl-CoA:monolignol transferase enzyme that enables incorporation of monolignol ferulates, for example, including p-coumaryl ferulate, coniferyl ferulate, and sinapyl ferulate, into the lignin of plants.

Inventors:
; ; ;
Publication Date:
Research Org.:
WISCONSIN ALUMNI RESEARCH FOUNDATION, Madison, WI (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1325010
Patent Number(s):
9,441,235
Application Number:
13/811,823
Assignee:
WISCONSIN ALUMNI RESEARCH FOUNDATION (Madison, WI) CHO
DOE Contract Number:
FC02-07ER64494
Resource Type:
Patent
Resource Relation:
Patent File Date: 2011 Jul 22
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Wilkerson, Curtis, Ralph, John, Withers, Saunia, and Mansfield, Shawn D. Feruloyl-CoA:monolignol transferase. United States: N. p., 2016. Web.
Wilkerson, Curtis, Ralph, John, Withers, Saunia, & Mansfield, Shawn D. Feruloyl-CoA:monolignol transferase. United States.
Wilkerson, Curtis, Ralph, John, Withers, Saunia, and Mansfield, Shawn D. 2016. "Feruloyl-CoA:monolignol transferase". United States. doi:. https://www.osti.gov/servlets/purl/1325010.
@article{osti_1325010,
title = {Feruloyl-CoA:monolignol transferase},
author = {Wilkerson, Curtis and Ralph, John and Withers, Saunia and Mansfield, Shawn D.},
abstractNote = {The invention relates to nucleic acids encoding a feruloyl-CoA:monolignol transferase and the feruloyl-CoA:monolignol transferase enzyme that enables incorporation of monolignol ferulates, for example, including p-coumaryl ferulate, coniferyl ferulate, and sinapyl ferulate, into the lignin of plants.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = 2016,
month = 9
}

Patent:

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  • The invention relates to nucleic acids encoding a feruloyl-CoA:monolignol transferase and the feruloyl-CoA:monolignol transferase enzyme that enables incorporation of monolignol ferulates, for example, including p-coumaryl ferulate, coniferyl ferulate, and sinapyl ferulate, into the lignin of plants.
  • The invention relates to isolated nucleic acids encoding a feruloyl-CoA:monolignol transferase and feruloyl-CoA:monolignol transferase enzymes. The isolated nucleic acids and/or the enzymes enable incorporation of monolignol ferulates into the lignin of plants, where such monolignol ferulates include, for example, p-coumaryl ferulate, coniferyl ferulate, and/or sinapyl ferulate. The invention also includes methods and plants that include nucleic acids encoding a feruloyl-CoA:monolignol transferase enzyme and/or feruloyl-CoA:monolignol transferase enzymes.
  • Coumarins belong to an important class of plant secondary metabolites. Feruloyl-CoA 6’-hydroxylase (F6’H), a 2-oxoglutarate dependent dioxygenase (2OGD), catalyzes a pivotal step in the biosynthesis of a simple coumarin scopoletin. In this study, we determined the 3-dimensional structure of the F6’H1 apo enzyme by X-ray crystallography. It is the first reported structure of a 2OGD enzyme involved in coumarin biosynthesis and closely resembles the structure of Arabidopsis thaliana anthocyanidin synthase. To better understand the mechanism of enzyme catalysis and substrate specificity, we also generated a homology model of a related ortho-hydroxylase (C 2’H) from sweet potato. By comparing these twomore » structures, we targeted two amino acid residues and verified their roles in substrate binding and specificity by site-directed mutagenesis.« less