Extracellular vesicles from Paracoccidioides pathogenic species transport polysaccharide and expose ligands for DC-SIGN receptors

da Silva, Roberta Peres; Heiss, Christian; Black, Ian; Azadi, Parastoo; Gerlach, Jared Q.; Travassos, Luiz R.; Joshi, Lokesh; Kilcoyne, Michelle; Puccia, Rosana

doi:10.1038/srep14213

Title: Extracellular vesicles from Paracoccidioides pathogenic species transport polysaccharide and expose ligands for DC-SIGN receptors

Journal Article · Mon Sep 21 00:00:00 EDT 2015 · Scientific Reports

DOI:https://doi.org/10.1038/srep14213· OSTI ID:1324971

da Silva, Roberta Peres ^[1]; Heiss, Christian ^[2]; Black, Ian ^[2]; Azadi, Parastoo ^[2]; Gerlach, Jared Q. ^[3]; Travassos, Luiz R. ^[1]; Joshi, Lokesh ^[3]; Kilcoyne, Michelle ^[4]; Puccia, Rosana ^[1]

Escola Paulista de Medicina-Univ. Federal de Sao Paulo, Sao Paulo (Brazil)
Univ. of Georgia, Athens, GA (United States)
National Centre for Biomedical Engineering Science, NUI Galway (Ireland)
National Centre for Biomedical Engineering Science, NUI Galway (Ireland); National Univ. of Ireland, Galway (Ireland)

Extracellular vesicles (EVs) mediate non-conventional transport of molecules across the fungal cell wall. We aimed at describing the carbohydrate composition and surface carbohydrate epitopes of EVs isolated from the pathogenic fungi Paracoccidioides brasiliensis and P. lutzii using standard procedures. Total EV carbohydrates were ethanol-precipitated from preparations depleted of lipids and proteins, then analyzed by chemical degradation, gas chromatography-mass spectrometry, nuclear magnetic resonance and size-exclusion chromatography. EV glycosyl residues of Glc, Man, and Gal comprised most probably two major components: a high molecular mass 4,6-α-glucan and a galactofuranosylmannan, possibly an oligomer, bearing a 2-α-Manp main chain linked to β-Galf (1,3) and α-Manp (1,6) end units. The results also suggested the presence of small amounts of a (1→6)- Manp polymer, (1→3)-glucan and (1→6)-glucan. Glycan microarrays allowed identification of EV surface lectin(s), while plant lectin microarray profiling revealed terminal Man and GlcNAc residues exposed at the EVs surface. Mammalian lectin microarray profiling showed that DC-SIGN receptors recognized surface carbohydrate in Paracoccidioides EVs. Our results suggest that oligosaccharides, cytoplasmic storage, and cell wall polysaccharides can be exported in fungal EVs, which also expose surface PAMPs and lectins. As a result, the role of these newly identified components in the interaction with the host remains to be unraveled.

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Research Organization:: Univ. of Georgia, Athens, GA (United States). Analytical Services & Training Lab. Complex Carbohydrate Research Center

Sponsoring Organization:: USDOE

Grant/Contract Number:: FG02-93ER20097

OSTI ID:: 1324971

Journal Information:: Scientific Reports, Vol. 5; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 49 works

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