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Title: Lignases and aldo-keto reductases for conversion of lignin-containing materials to fermentable products

Abstract

Termites have specialized digestive systems that overcome the lignin barrier in wood to release fermentable simple sugars. Using the termite Reticulitermes flavipes and its gut symbionts, high-throughput titanium pyrosequencing and proteomics approaches experimentally compared the effects of lignin-containing diets on host-symbiont digestome composition. Proteomic investigations and functional digestive studies with recombinant lignocellulases conducted in parallel provided strong evidence of congruence at the transcription and translational levels and provide enzymatic strategies for overcoming recalcitrant lignin barriers in biofuel feedstocks. Briefly described, therefore, the disclosure provides a system for generating a fermentable product from a lignified plant material, the system comprising a cooperating series of at least two catalytically active polypeptides, where said catalytically active polypeptides are selected from the group consisting of: cellulase Cell-1, .beta.-glu cellulase, an aldo-keto-reductase, a catalase, a laccase, and an endo-xylanase.

Inventors:
;
Publication Date:
Research Org.:
PURDUE RESEARCH FOUNDATION, West Lafayette, IN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1324695
Patent Number(s):
9,441,256
Application Number:
14/379,990
Assignee:
PURDUE RESEARCH FOUNDATION (West Lafayette, IN)
DOE Contract Number:  
FC03-97ER62402
Resource Type:
Patent
Resource Relation:
Patent File Date: 2013 Feb 11
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS

Citation Formats

Scharf, Michael, and Sethi, Amit. Lignases and aldo-keto reductases for conversion of lignin-containing materials to fermentable products. United States: N. p., 2016. Web.
Scharf, Michael, & Sethi, Amit. Lignases and aldo-keto reductases for conversion of lignin-containing materials to fermentable products. United States.
Scharf, Michael, and Sethi, Amit. Tue . "Lignases and aldo-keto reductases for conversion of lignin-containing materials to fermentable products". United States. https://www.osti.gov/servlets/purl/1324695.
@article{osti_1324695,
title = {Lignases and aldo-keto reductases for conversion of lignin-containing materials to fermentable products},
author = {Scharf, Michael and Sethi, Amit},
abstractNote = {Termites have specialized digestive systems that overcome the lignin barrier in wood to release fermentable simple sugars. Using the termite Reticulitermes flavipes and its gut symbionts, high-throughput titanium pyrosequencing and proteomics approaches experimentally compared the effects of lignin-containing diets on host-symbiont digestome composition. Proteomic investigations and functional digestive studies with recombinant lignocellulases conducted in parallel provided strong evidence of congruence at the transcription and translational levels and provide enzymatic strategies for overcoming recalcitrant lignin barriers in biofuel feedstocks. Briefly described, therefore, the disclosure provides a system for generating a fermentable product from a lignified plant material, the system comprising a cooperating series of at least two catalytically active polypeptides, where said catalytically active polypeptides are selected from the group consisting of: cellulase Cell-1, .beta.-glu cellulase, an aldo-keto-reductase, a catalase, a laccase, and an endo-xylanase.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {9}
}

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