Proteomic-based stable isotope probing reveals taxonomically Distinct Patterns in Amino Acid Assimilation by Coastal Marine Bacterioplankton

Bryson, Samuel; Li, Zhou; Pett-Ridge, Jennifer; Mayali, Xavier; Pan, Chongle; Mueller, Ryan S.

doi:10.1128/mSystems.00027-15

Title: Proteomic-based stable isotope probing reveals taxonomically Distinct Patterns in Amino Acid Assimilation by Coastal Marine Bacterioplankton

Journal Article · Tue Apr 26 00:00:00 EDT 2016 · mSystems

DOI:https://doi.org/10.1128/mSystems.00027-15· OSTI ID:1324077

Bryson, Samuel ^[1]; Li, Zhou ^[2]; Pett-Ridge, Jennifer ^[3]; Mayali, Xavier ^[3]; Pan, Chongle ^[4]; Mueller, Ryan S. ^[1]

Oregon State Univ., Corvallis, OR (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Heterotrophic marine bacterioplankton are a critical component of the carbon cycle, processing nearly a quarter of annual global primary production, yet defining how substrate utilization preferences and resource partitioning structure these microbial communities remains a challenge. In this study, we utilized proteomics-based stable isotope probing (proteomic SIP) to characterize the assimilation of amino acids by coastal marine bacterioplankton populations. We incubated microcosms of seawater collected from Newport, OR and Monterey Bay, CA with 1 M ¹³C-amino acids for 15 and 32 hours. Subsequent analysis of ¹³C incorporation into protein biomass quantified the frequency and extent of isotope enrichment for identified proteins. Using these metrics we tested whether amino acid assimilation patterns were different for specific bacterioplankton populations. Proteins associated with Rhodobacterales and Alteromonadales tended to have a significantly high number of tandem mass spectra from ¹³C-enriched peptides, while Flavobacteriales and SAR11 proteins generally had significantly low numbers of ¹³C-enriched spectra. Rhodobacterales proteins associated with amino acid transport and metabolism had an increased frequency of ¹³C-enriched spectra at time-point 2, while Alteromonadales ribosomal proteins were ¹³C- enriched across time-points. Overall, proteomic SIP facilitated quantitative comparisons of dissolved free amino acids assimilation by specific taxa, both between sympatric populations and between protein functional groups within discrete populations, allowing an unprecedented examination of population-level metabolic responses to resource acquisition in complex microbial communities.

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Research Organization:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Oak Ridge Leadership Computing Facility (OLCF)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1324077

Journal Information:: mSystems, Vol. 1, Issue 2; ISSN 2379-5077

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 25 works

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