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Title: Assessment of microcrystal quality by transmission electron microscopy for efficient serial femtosecond crystallography

Authors:
; ; ; ; ; ; ; ;
Publication Date:
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1324012
Grant/Contract Number:
0006-15; AC02-76SF00515
Resource Type:
Journal Article: Publisher's Accepted Manuscript
Journal Name:
Archives of Biochemistry and Biophysics
Additional Journal Information:
Journal Volume: 602; Journal Issue: C; Related Information: CHORUS Timestamp: 2017-10-03 21:33:41; Journal ID: ISSN 0003-9861
Publisher:
Elsevier
Country of Publication:
United States
Language:
English

Citation Formats

Barnes, Christopher O., Kovaleva, Elena G., Fu, Xiaofeng, Stevenson, Hilary P., Brewster, Aaron S., DePonte, Daniel P., Baxter, Elizabeth L., Cohen, Aina E., and Calero, Guillermo. Assessment of microcrystal quality by transmission electron microscopy for efficient serial femtosecond crystallography. United States: N. p., 2016. Web. doi:10.1016/j.abb.2016.02.011.
Barnes, Christopher O., Kovaleva, Elena G., Fu, Xiaofeng, Stevenson, Hilary P., Brewster, Aaron S., DePonte, Daniel P., Baxter, Elizabeth L., Cohen, Aina E., & Calero, Guillermo. Assessment of microcrystal quality by transmission electron microscopy for efficient serial femtosecond crystallography. United States. doi:10.1016/j.abb.2016.02.011.
Barnes, Christopher O., Kovaleva, Elena G., Fu, Xiaofeng, Stevenson, Hilary P., Brewster, Aaron S., DePonte, Daniel P., Baxter, Elizabeth L., Cohen, Aina E., and Calero, Guillermo. 2016. "Assessment of microcrystal quality by transmission electron microscopy for efficient serial femtosecond crystallography". United States. doi:10.1016/j.abb.2016.02.011.
@article{osti_1324012,
title = {Assessment of microcrystal quality by transmission electron microscopy for efficient serial femtosecond crystallography},
author = {Barnes, Christopher O. and Kovaleva, Elena G. and Fu, Xiaofeng and Stevenson, Hilary P. and Brewster, Aaron S. and DePonte, Daniel P. and Baxter, Elizabeth L. and Cohen, Aina E. and Calero, Guillermo},
abstractNote = {},
doi = {10.1016/j.abb.2016.02.011},
journal = {Archives of Biochemistry and Biophysics},
number = C,
volume = 602,
place = {United States},
year = 2016,
month = 7
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at 10.1016/j.abb.2016.02.011

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  • Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment. We introduce a double-flow focusing nozzle to meet this challenge, with significantly reduced sample consumption, while improving jet stability over previous generations of nozzles. We demonstrate its use to determine the first room-temperature structure of RNA polymerase II at high resolution, revealing new structural details. Furthermore, the double flow-focusing nozzles were successfully tested with three other protein samples and the first room temperature structure of an extradiol ring-cleaving dioxygenase was solved by utilizing the improvedmore » operation and characteristics of these devices.« less
  • X-ray free-electron lasers deliver intense femtosecond pulses that promise to yield high resolution diffraction data of nanocrystals before the destruction of the sample by radiation damage. Diffraction intensities of lysozyme nanocrystals collected at the Linac Coherent Light Source using 2 keV photons were used for structure determination by molecular replacement and analyzed for radiation damage as a function of pulse length and fluence. Signatures of radiation damage are observed for pulses as short as 70 fs. Parametric scaling used in conventional crystallography does not account for the observed effects.
  • Please check the README file inside the metadata directory for more information about the dataset.
  • The 18R martensite phase was observed in the b.c.c. matrix of an Fe-25.8 wt% Mn-7.4 wt% Al-0.11 wt% C alloy, after cooling from high temperature. The crystal structure is determined from the selected area diffraction (SAD) and high resolution imaging techniques. The 18R martensite has an orthorhombic lattice with lattice constants a = 0.448 nm, b= 0.259 nm and c = 3.865 nm and is best described as an 18R(4[bar 2])[sub 3] rather than an 18R(5[bar 1])[sub 3] structure. The expression of 18R(4[bar 2])[sub 3] and 18R(5[bar 1])[sub 3] comes from the combined notation of Ramsdell and Zhdanov [Nishiyama, Martensiticmore » Transformation, p. 75 (1978)]. The stacking fault density relative to the 18R(4[bar 2])[sub 3] structure is determined from high resolution imaging to be 0.096. Martensite crystallographic parameters such as orientation relationship, habit plane, shape strain direction and magnitude of lattice invariant shear are calculated using the CRAB theory, in good agreement with experimental observations. The Burgers vector of anticoherency dislocations and the shape strain direction were confirmed using the two beam technique and computer image simulation. The observed interfacial dislocation structure and choice of lattice correspondence are discussed in terms of near-CSL concepts.« less