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Title: Cortisol and testosterone accumulation in a low pH recirculating aquaculture system for rainbow trout ( Oncorhynchus mykiss )

Authors:
 [1];  [2];  [3];  [2];  [3]
  1. Aquaculture and Fisheries Group, Wageningen University, Wageningen the Netherlands, CCMAR - Centro de Ciências do Mar, Universidade do Algarve, Faro Portugal
  2. CCMAR - Centro de Ciências do Mar, Universidade do Algarve, Faro Portugal
  3. Aquaculture and Fisheries Group, Wageningen University, Wageningen the Netherlands
Publication Date:
Sponsoring Org.:
USDOE Office of Nuclear Energy (NE), Fuel Cycle Technologies (NE-5)
OSTI Identifier:
1321030
Resource Type:
Journal Article: Published Article
Journal Name:
Aquaculture Research
Additional Journal Information:
Journal Volume: 48; Journal Issue: 7; Related Information: CHORUS Timestamp: 2017-06-12 03:03:43; Journal ID: ISSN 1355-557X
Publisher:
Wiley-Blackwell
Country of Publication:
Country unknown/Code not available
Language:
English

Citation Formats

Mota, Vasco C., Martins, Catarina I. M., Eding, Ep H., Canário, Adelino V. M., and Verreth, Johan A. J. Cortisol and testosterone accumulation in a low pH recirculating aquaculture system for rainbow trout ( Oncorhynchus mykiss ). Country unknown/Code not available: N. p., 2016. Web. doi:10.1111/are.13184.
Mota, Vasco C., Martins, Catarina I. M., Eding, Ep H., Canário, Adelino V. M., & Verreth, Johan A. J. Cortisol and testosterone accumulation in a low pH recirculating aquaculture system for rainbow trout ( Oncorhynchus mykiss ). Country unknown/Code not available. doi:10.1111/are.13184.
Mota, Vasco C., Martins, Catarina I. M., Eding, Ep H., Canário, Adelino V. M., and Verreth, Johan A. J. 2016. "Cortisol and testosterone accumulation in a low pH recirculating aquaculture system for rainbow trout ( Oncorhynchus mykiss )". Country unknown/Code not available. doi:10.1111/are.13184.
@article{osti_1321030,
title = {Cortisol and testosterone accumulation in a low pH recirculating aquaculture system for rainbow trout ( Oncorhynchus mykiss )},
author = {Mota, Vasco C. and Martins, Catarina I. M. and Eding, Ep H. and Canário, Adelino V. M. and Verreth, Johan A. J.},
abstractNote = {},
doi = {10.1111/are.13184},
journal = {Aquaculture Research},
number = 7,
volume = 48,
place = {Country unknown/Code not available},
year = 2016,
month = 8
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at 10.1111/are.13184

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  • Species differences in physiological and biochemical attributes exist even among closely related species and may underlie species-specific sensitivity to toxicants. Rainbow trout (RT) are more sensitive than brook trout (BT) to the teratogenic effects of selenium (Se), but it is not known whether all tissues exhibit this pattern of vulnerability. In this study, primary cultures of RT and BT adrenocortical cells were exposed to selenite (Na{sub 2}SO{sub 3}) and selenomethionine (Se-Met) to compare cell viability and ACTH-stimulated cortisol secretion in the two fish species. Cortisol, the primary stress hormone in fish, facilitates maintenance of homeostasis when fish are exposed tomore » stressors, including toxicants. Cell viability was not affected by Se, but selenite impaired cortisol secretion, while Se-Met did not (RT and BT EC{sub 50} > 2000 mg/L). RT cells were more sensitive (EC{sub 50} = 8.7 mg/L) to selenite than BT cells (EC{sub 50} = 90.4 mg/L). To identify the targets where Se disrupts cortisol synthesis, selenite-impaired RT and BT cells were stimulated with ACTH, dbcAMP, OH-cholesterol, and pregnenolone. Selenite acted at different steps in the cortisol biosynthesis pathway in RT and BT cells, confirming a species-specific toxicity mechanism. To test the hypothesis that oxidative stress mediates Se-induced toxicity, selenite-impaired RT cells were exposed to NAC, BSO and antioxidants (DETCA, ATA, Vit A, and Vit E). Inhibition of SOD by DETCA enhanced selenite-induced cortisol impairment, indicating that oxidative stress plays a role in Se toxicity; however, modifying GSH content of the cells did not have an effect. The results of this study, with two closely related salmonids, provided additional evidence for species-specific differences in sensitivity to Se which should be considered when setting thresholds and water quality guidelines. - Research Highlights: > We investigated species-specific sensitivity to Se in trout adrenocortical cells. > Selenite, not Se-Met, disrupts cortisol secretion in trout adrenocortical cells. > Rainbow trout cells are more sensitive than brook trout cells to selenite toxicity. > Superoxide dismutase may protect adrenocortical cells from selenite toxicity.« less
  • Olfactory epithelial structure and olfactory bulb neurophysiological responses were measured in chinook salmon and rainbow trout in response to 25 to 300 {micro}g copper (Cu)/L. Using confocal laser scanning microscopy, the number of olfactory receptors was significantly reduced in chinook salmon exposed to {ge}50 {micro}g Cu/L and in rainbow trout exposed to {ge}200 {micro}g cu/L for 1 h. The number of receptors was significantly reduced in both species following exposure to 25 {micro}g Cu/L for 4 h. Transmission electron microscopy of olfactory epithelial tissue indicated that the loss of receptors was from cellular necrosis. Olfactory bulk electroencephalogram (EEG) responses tomore » 10{sup {minus}3} M L-serine were initially reduced by all Cu concentrations but were virtually eliminated in chinook salmon exposed to {ge}50 {micro}g Cu/L and in rainbow trout exposed to {ge}200 {micro}g Cu/L within 1 h of exposure. Following Cu exposure, EEG response recovery rates were slower in fish exposed to higher Cu concentrations. The higher sensitivity of the chinook salmon olfactory system to Cu-induced histological damage and neurophysiological impairment parallels the relative species sensitivity observed in behavioral avoidance experiments. This difference in species sensitivity may reduce the survival and reproductive potential of chinook salmon compared with that of rainbow trout in Cu-contaminated waters.« less
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