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Title: Using genotyping by sequencing to map two novel anthracnose resistance Loci in Sorghum bicolor

Abstract

Colletotrichum sublineola is an aggressive fungal pathogen that causes anthracnose in sorghum [ Sorghum bicolor (L.) Moench]. The obvious symptoms of anthracnose are leaf blight and stem rot. Sorghum, the fifth most widely grown cereal crop in the world, can be highly susceptible to the disease, most notably in hot and humid environments. In the southeastern United States the acreage of sorghum has been increasing steadily in recent years, spurred by growing interest in producing biofuels, bio-based products, and animal feed. Resistance to anthracnose is, therefore, of paramount importance for successful sorghum production in this region. To identify anthracnose resistance loci present in the highly resistant cultivar ‘Bk7’, a biparental mapping population of F 3:4 and F 4:5 sorghum lines was generated by crossing ‘Bk7’ with the susceptible inbred ‘Early Hegari-Sart’. Lines were phenotyped in three environments and in two different years following natural infection. The population was genotyped by sequencing. Following a stringent custom filtering protocol, totals of 5186 and 2759 informative SNP markers were identified in the two populations. Segregation data and association analysis identified resistance loci on chromosomes 7 and 9, with the resistance alleles derived from ‘Bk7’. Both loci contain multiple classes of defense-related genes basedmore » on sequence similarity and gene ontologies. In addition, genetic analysis following an independent selection experiment of lines derived from a cross between ‘Bk7’ and sweet sorghum ‘Mer81-4’ narrowed the resistance locus on chromosome 9 substantially, validating this QTL. As observed in other species, sorghum appears to have regions of clustered resistance genes. Further characterization of these regions will facilitate the development of novel germplasm with resistance to anthracnose and other diseases.« less

Authors:
 [1]; ORCiD logo [1];  [1]; ORCiD logo [1]
  1. Univ. of Florida, Gainesville, FL (United States)
Publication Date:
Research Org.:
Univ. of Florida, Gainesville, FL (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1313705
Grant/Contract Number:  
SC0014439
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
G3
Additional Journal Information:
Journal Volume: 6; Journal Issue: 7; Journal ID: ISSN 2160-1836
Publisher:
Genetics Society of America
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; anthracnose; Colletotrichum sublineola; GBS; sorghum

Citation Formats

Felderhoff, Terry J., McIntyre, Lauren M., Saballos, Ana, and Vermerris, Wilfred. Using genotyping by sequencing to map two novel anthracnose resistance Loci in Sorghum bicolor. United States: N. p., 2016. Web. doi:10.1534/g3.116.030510.
Felderhoff, Terry J., McIntyre, Lauren M., Saballos, Ana, & Vermerris, Wilfred. Using genotyping by sequencing to map two novel anthracnose resistance Loci in Sorghum bicolor. United States. doi:10.1534/g3.116.030510.
Felderhoff, Terry J., McIntyre, Lauren M., Saballos, Ana, and Vermerris, Wilfred. Wed . "Using genotyping by sequencing to map two novel anthracnose resistance Loci in Sorghum bicolor". United States. doi:10.1534/g3.116.030510. https://www.osti.gov/servlets/purl/1313705.
@article{osti_1313705,
title = {Using genotyping by sequencing to map two novel anthracnose resistance Loci in Sorghum bicolor},
author = {Felderhoff, Terry J. and McIntyre, Lauren M. and Saballos, Ana and Vermerris, Wilfred},
abstractNote = {Colletotrichum sublineola is an aggressive fungal pathogen that causes anthracnose in sorghum [Sorghum bicolor (L.) Moench]. The obvious symptoms of anthracnose are leaf blight and stem rot. Sorghum, the fifth most widely grown cereal crop in the world, can be highly susceptible to the disease, most notably in hot and humid environments. In the southeastern United States the acreage of sorghum has been increasing steadily in recent years, spurred by growing interest in producing biofuels, bio-based products, and animal feed. Resistance to anthracnose is, therefore, of paramount importance for successful sorghum production in this region. To identify anthracnose resistance loci present in the highly resistant cultivar ‘Bk7’, a biparental mapping population of F3:4 and F4:5 sorghum lines was generated by crossing ‘Bk7’ with the susceptible inbred ‘Early Hegari-Sart’. Lines were phenotyped in three environments and in two different years following natural infection. The population was genotyped by sequencing. Following a stringent custom filtering protocol, totals of 5186 and 2759 informative SNP markers were identified in the two populations. Segregation data and association analysis identified resistance loci on chromosomes 7 and 9, with the resistance alleles derived from ‘Bk7’. Both loci contain multiple classes of defense-related genes based on sequence similarity and gene ontologies. In addition, genetic analysis following an independent selection experiment of lines derived from a cross between ‘Bk7’ and sweet sorghum ‘Mer81-4’ narrowed the resistance locus on chromosome 9 substantially, validating this QTL. As observed in other species, sorghum appears to have regions of clustered resistance genes. Further characterization of these regions will facilitate the development of novel germplasm with resistance to anthracnose and other diseases.},
doi = {10.1534/g3.116.030510},
journal = {G3},
issn = {2160-1836},
number = 7,
volume = 6,
place = {United States},
year = {2016},
month = {5}
}

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    Works referencing / citing this record:

    QTL mapping reveals genetic determinants of fungal disease resistance in the wild lentil species Lens ervoides
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    Genome-Wide Association Mapping of Anthracnose ( Colletotrichum sublineolum ) Resistance in the U.S. Sorghum Association Panel
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