Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography

Nogly, Przemyslaw; Panneels, Valerie; Nelson, Garrett; Gati, Cornelius; Kimura, Tetsunari; Milne, Christopher; Milathianaki, Despina; Kubo, Minoru; Wu, Wenting; Conrad, Chelsie; Coe, Jesse; Bean, Richard; Zhao, Yun; Bath, Petra; Dods, Robert; Harimoorthy, Rajiv; Beyerlein, Kenneth R.; Rheinberger, Jan; James, Daniel; DePonte, Daniel; Li, Chufeng; Sala, Leonardo; Williams, Garth J.; Hunter, Mark S.; Koglin, Jason E.; Berntsen, Peter; Nango, Eriko; Iwata, So; Chapman, Henry N.; Fromme, Petra; Frank, Matthias; Abela, Rafael; Boutet, Sebastien; Barty, Anton; White, Thomas A.; Weierstall, Uwe; Spence, John; Neutze, Richard; Schertler, Gebhard; Standfuss, Jorg

doi:10.1038/ncomms12314

Title: Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography

Journal Article · Mon Aug 22 00:00:00 EDT 2016 · Nature Communications

DOI:https://doi.org/10.1038/ncomms12314· OSTI ID:1313008

^[1]; Panneels, Valerie ^[1]; Nelson, Garrett ^[2]; Gati, Cornelius ^[3]; Kimura, Tetsunari ^[4];

^[1]; Milathianaki, Despina ^[5]; Kubo, Minoru ^[6]; Wu, Wenting ^[1]; Conrad, Chelsie ^[2]; Coe, Jesse ^[2]; Bean, Richard ^[3]; Zhao, Yun ^[2]; Bath, Petra ^[7]; Dods, Robert ^[7]; Harimoorthy, Rajiv ^[7]; Beyerlein, Kenneth R. ^[3]; Rheinberger, Jan ^[1];

^[1]; DePonte, Daniel ^[5] more »

Paul Scherrer Inst. (PSI), Villigen (Switzerland)
Arizona State Univ., Tempe, AZ (United States)
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
RIKEN SPring-8 Center, Hyogo (Japan)
SLAC National Accelerator Lab., Menlo Park, CA (United States)
RIKEN SPring-8 Center, Hyogo (Japan); PRESTO, JST, Saitama (Japan)
Univ. of Gothenburg, Gothenburg (Sweden)
RIKEN/SPring-8 Center, Hyogo (Japan)
RIKEN/SPring-8 Center, Hyogo (Japan); Kyoto Univ., Kyoto (Japan)
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Univ. of Hamburg, Hamburg (Germany)
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Paul Scherrer Inst. (PSI), Villigen (Switzerland); ETH Zurich, Zurich (Switzerland)

Serial femtosecond crystallography (SFX) using X-ray free-electron laser sources is an emerging method with considerable potential for time-resolved pump-probe experiments. Here we present a lipidic cubic phase SFX structure of the light-driven proton pump bacteriorhodopsin (bR) to 2.3 Å resolution and a method to investigate protein dynamics with modest sample requirement. Time-resolved SFX (TR-SFX) with a pump-probe delay of 1 ms yields difference Fourier maps compatible with the dark to M state transition of bR. Importantly, the method is very sample efficient and reduces sample consumption to about 1 mg per collected time point. Accumulation of M intermediate within the crystal lattice is confirmed by time-resolved visible absorption spectroscopy. This study provides an important step towards characterizing the complete photocycle dynamics of retinal proteins and demonstrates the feasibility of a sample efficient viscous medium jet for TR-SFX.

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Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States); Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE National Nuclear Security Administration (NNSA)

Grant/Contract Number:: AC02-76SF00515; AC52-07NA27344

OSTI ID:: 1313008

Alternate ID(s):: OSTI ID: 1959522

Report Number(s):: LLNL-JRNL-844106; ncomms12314

Journal Information:: Nature Communications, Vol. 7; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 69 works

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Time-resolved x-ray crystallography capture of a slow reaction tetrahydrofolate intermediate Cao, Hongnan; Skolnick, Jeffrey Structural Dynamics, Vol. 6, Issue 2 https://doi.org/10.1063/1.5086436	journal	March 2019
The serial millisecond crystallography instrument at the Australian Synchrotron incorporating the “Lipidico” injector Berntsen, P.; Hadian Jazi, M.; Kusel, M. Review of Scientific Instruments, Vol. 90, Issue 8 https://doi.org/10.1063/1.5104298	journal	August 2019
Proton uptake mechanism in bacteriorhodopsin captured by serial synchrotron crystallography Weinert, Tobias; Skopintsev, Petr; James, Daniel Science, Vol. 365, Issue 6448 https://doi.org/10.1126/science.aaw8634	journal	July 2019
Retinal isomerization in bacteriorhodopsin captured by a femtosecond x-ray laser Nogly, Przemyslaw; Weinert, Tobias; James, Daniel Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2018-05837	text	January 2018
Strategies for sample delivery for femtosecond crystallography Martiel, Isabelle; Müller-Werkmeister, Henrike M.; Cohen, Aina E. International Union of Crystallography (IUCr) https://doi.org/10.1107/s2059798318017953/ba5296sup1.pdf	text	January 2019
Photoswitching mechanism of a fluorescent protein revealed by time-resolved crystallography and transient absorption spectroscopy Woodhouse, Joyce; Nass Kovacs, Gabriela; Coquelle, Nicolas Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-020-14537-0	journal	February 2020
Short-wavelength free-electron laser sources and science: a review Seddon, E. A.; Clarke, J. A.; Dunning, D. J. Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2018-00596	text	January 2017
A guide to sample delivery systems for serial crystallography Zhao, Feng‐Zhu; Zhang, Bin; Yan, Er‐Kai The FEBS Journal, Vol. 286, Issue 22 https://doi.org/10.1111/febs.15099	journal	August 2019
SPIND : a reference-based auto-indexing algorithm for sparse serial crystallography data Li, Chufeng; Li, Xuanxuan; Kirian, Richard IUCrJ, Vol. 6, Issue 1 https://doi.org/10.1107/s2052252518014951	journal	January 2019
Serial millisecond crystallography for routine room-temperature structure determination at synchrotrons Weinert, Tobias; Olieric, Natacha; Cheng, Robert Nature Communications, Vol. 8, Issue 1 https://doi.org/10.1038/s41467-017-00630-4	journal	September 2017
Serial millisecond crystallography for routine room-temperature structure determination at synchrotrons Weinert, Tobias; Olieric, Natacha; Cheng, Robert ETH Zurich https://doi.org/10.3929/ethz-b-000191628	text	January 2017
Orientation of non-spherical protonated water clusters revealed by infrared absorption dichroism Daldrop, Jan O.; Saita, Mattia; Heyden, Matthias Nature Communications, Vol. 9, Issue 1 https://doi.org/10.1038/s41467-017-02669-9	journal	January 2018
Temperature dependence of protein-water interactions in a gated yeast aquaporin Aponte-Santamaría, Camilo; Fischer, Gerhard; Båth, Petra Scientific Reports, Vol. 7, Issue 1 https://doi.org/10.1038/s41598-017-04180-z	journal	June 2017