Characterization of DWARF14 Genes in Populus

Zheng, Kaijie; Wang, Xiaoping; Weighill, Deborah A.; Guo, Hao-Bo; Xie, Meng; Yang, Yongil; Yang, Jun; Wang, Shucai; Jacobson, Daniel A.; Guo, Hong; Muchero, Wellington; Tuskan, Gerald A.; Chen, Jin-Gui

doi:10.1038/srep21593

Title: Characterization of DWARF14 Genes in Populus

Journal Article · Mon Feb 15 00:00:00 EST 2016 · Scientific Reports

DOI:https://doi.org/10.1038/srep21593· OSTI ID:1259854

Zheng, Kaijie ^[1]; Wang, Xiaoping ^[1]; Weighill, Deborah A. ^[2]; Guo, Hao-Bo ^[3]; Xie, Meng ^[4]; Yang, Yongil ^[4]; Yang, Jun ^[5]; Wang, Shucai ^[6]; Jacobson, Daniel A. ^[7]; Guo, Hong ^[3]; Muchero, Wellington ^[4]; Tuskan, Gerald A. ^[4]; Chen, Jin-Gui ^[4]

Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division; Northeast Normal Univ., Changchun (China)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division; Univ. of Tennessee, Knoxville, TN (United States). Bredesen Center for Interdisciplinary Research and Graduate Education
Univ. of Tennessee, Knoxville, TN (United States). Department of Biochemistry and Cellular and Molecular Biology
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division; Chinese Academy of Sciences (CAS), Shanghai (China). Shanghai Inst. for Biological Sciences
Northeast Normal Univ., Changchun (China)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division; Univ. of Tennessee, Knoxville, TN (United States). Bredesen Center for Interdisciplinary Research and Graduate Education; Univ. of Tennessee, Knoxville, TN (United States). Genome Science and Technology Program

We report that strigolactones are a new class of plant hormones regulating shoot branching and symbiotic interactions with arbuscular mycorrhizal fungi. Studies of branching mutants in herbaceous plants have identified several key genes involved in strigolactone biosynthesis or signaling. The strigolactone signal is perceived by a member of the α/β-fold hydrolase superfamily, known as DWARF14 (D14). However, little is known about D14 genes in the woody perennial plants. Here we report the identification of D14 homologs in the model woody plant Populus trichocarpa. We showed that there are two D14 homologs in P. trichocarpa, designated as PtD14a and PtD14b that are over 95% similar at the amino acid level. Expression analysis indicated that the transcript level of PtD14a is generally more abundant than that of PtD14b. However, only PtD14a was able to complement Arabidopsis d14 mutants, suggesting that PtD14a is the functional D14 ortholog. Amino acid alignment and structural modeling revealed substitutions of several highly conserved amino acids in the PtD14b protein including a phenylalanine near the catalytic triad of D14 proteins. In conclusion, this study lays a foundation for further characterization of strigolactone pathway and its functions in the woody perennial plants.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Oak Ridge Leadership Computing Facility (OLCF)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); Chinese Academy of Sciences (CAS)

Grant/Contract Number:: AC05-00OR22725; 201019

OSTI ID:: 1259854

Alternate ID(s):: OSTI ID: 1311227

Journal Information:: Scientific Reports, Vol. 6; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 20 works

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