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Title: Applications of capillary electrophoresis and laser-induced fluorescence detection to the analysis of trace species: From single cells to single molecules

Abstract

This Ph.D. Thesis describes several separation and detection schemes for the analysis of small volume and amount of samples, such as intracellular components and single enzymes developed during research. Indirect Laser-induced fluorescence detection and capillary electrophoresis were used to quantify lactate and pyruvate in single red blood cells. The assay of specific enzyme activities was achieved by monitoring the highly fluorescent enzymatic reaction product, NADH. LDH activity was found not to be a unique marker for diagnosis of leukemia. Reactions of single LDH-1 molecules were investigated by monitoring the reaction product with LIF detection.

Authors:
 [1]
  1. Iowa State Univ., Ames, IA (United States)
Publication Date:
Research Org.:
Ames Lab., IA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
130612
Report Number(s):
IS-T-1715
ON: DE96002247; TRN: 95:008303
DOE Contract Number:  
W-7405-ENG-82
Resource Type:
Thesis/Dissertation
Resource Relation:
Other Information: TH: Thesis (Ph.D.); PBD: 1995
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ENZYMES; EVALUATION; TRACE AMOUNTS; ANIMAL CELLS; BIOCHEMISTRY; CELL CONSTITUENTS; ELECTROPHORESIS; FLUORESCENCE; BLOOD CELLS

Citation Formats

Xue, Qifeng. Applications of capillary electrophoresis and laser-induced fluorescence detection to the analysis of trace species: From single cells to single molecules. United States: N. p., 1995. Web. doi:10.2172/130612.
Xue, Qifeng. Applications of capillary electrophoresis and laser-induced fluorescence detection to the analysis of trace species: From single cells to single molecules. United States. doi:10.2172/130612.
Xue, Qifeng. Tue . "Applications of capillary electrophoresis and laser-induced fluorescence detection to the analysis of trace species: From single cells to single molecules". United States. doi:10.2172/130612. https://www.osti.gov/servlets/purl/130612.
@article{osti_130612,
title = {Applications of capillary electrophoresis and laser-induced fluorescence detection to the analysis of trace species: From single cells to single molecules},
author = {Xue, Qifeng},
abstractNote = {This Ph.D. Thesis describes several separation and detection schemes for the analysis of small volume and amount of samples, such as intracellular components and single enzymes developed during research. Indirect Laser-induced fluorescence detection and capillary electrophoresis were used to quantify lactate and pyruvate in single red blood cells. The assay of specific enzyme activities was achieved by monitoring the highly fluorescent enzymatic reaction product, NADH. LDH activity was found not to be a unique marker for diagnosis of leukemia. Reactions of single LDH-1 molecules were investigated by monitoring the reaction product with LIF detection.},
doi = {10.2172/130612},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1995},
month = {9}
}

Thesis/Dissertation:
Other availability
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