skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Recoding method that removes inhibitory sequences and improves HIV gene expression

Abstract

The invention relates to inhibitory nucleotide signal sequences or "INS" sequences in the genomes of lentiviruses. In particular the invention relates to the AGG motif present in all viral genomes. The AGG motif may have an inhibitory effect on a virus, for example by reducing the levels of, or maintaining low steady-state levels of, viral RNAs in host cells, and inducing and/or maintaining in viral latency. In one aspect, the invention provides vaccines that contain, or are produced from, viral nucleic acids in which the AGG sequences have been mutated. In another aspect, the invention provides methods and compositions for affecting the function of the AGG motif, and methods for identifying other INS sequences in viral genomes.

Inventors:
; ; ; ;
Publication Date:
Research Org.:
Institute of Advanced Study, Princeton, NJ (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1303730
Patent Number(s):
9,422,342
Application Number:
12/373,605
Assignee:
Institute of Advanced Study (Princeton, NJ) CHO
DOE Contract Number:  
FG02-90ER40542
Resource Type:
Patent
Resource Relation:
Patent File Date: 2007 Jul 12
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES

Citation Formats

Rabadan, Raul, Krasnitz, Michael, Robins, Harlan, Witten, Daniela, and Levine, Arnold. Recoding method that removes inhibitory sequences and improves HIV gene expression. United States: N. p., 2016. Web.
Rabadan, Raul, Krasnitz, Michael, Robins, Harlan, Witten, Daniela, & Levine, Arnold. Recoding method that removes inhibitory sequences and improves HIV gene expression. United States.
Rabadan, Raul, Krasnitz, Michael, Robins, Harlan, Witten, Daniela, and Levine, Arnold. Tue . "Recoding method that removes inhibitory sequences and improves HIV gene expression". United States. doi:. https://www.osti.gov/servlets/purl/1303730.
@article{osti_1303730,
title = {Recoding method that removes inhibitory sequences and improves HIV gene expression},
author = {Rabadan, Raul and Krasnitz, Michael and Robins, Harlan and Witten, Daniela and Levine, Arnold},
abstractNote = {The invention relates to inhibitory nucleotide signal sequences or "INS" sequences in the genomes of lentiviruses. In particular the invention relates to the AGG motif present in all viral genomes. The AGG motif may have an inhibitory effect on a virus, for example by reducing the levels of, or maintaining low steady-state levels of, viral RNAs in host cells, and inducing and/or maintaining in viral latency. In one aspect, the invention provides vaccines that contain, or are produced from, viral nucleic acids in which the AGG sequences have been mutated. In another aspect, the invention provides methods and compositions for affecting the function of the AGG motif, and methods for identifying other INS sequences in viral genomes.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Aug 23 00:00:00 EDT 2016},
month = {Tue Aug 23 00:00:00 EDT 2016}
}

Patent:

Save / Share:

Works referenced in this record:

Relative contributions of codon usage, promoter efficiency and leader sequence to the antigen expression and immunogenicity of HIV-1 Env DNA vaccine
journal, May 2006