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Method for genetic identification of unknown organisms

Patent ·
OSTI ID:1303664
; ; ; ;
A method of rapid, genome and proteome based identification of unknown pathogenic or non-pathogenic organisms in a complex sample. The entire sample is analyzed by creating millions of emulsion encapsulated microdroplets, each containing a single pathogenic or non-pathogenic organism sized particle and appropriate reagents for amplification. Following amplification, the amplified product is analyzed.
Research Organization:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
W-7405-ENG-48;
Assignee:
Lawrence Livermore National Security, LLC (Livermore, CA)
Patent Number(s):
9,422,586
Application Number:
11/818,623
OSTI ID:
1303664
Country of Publication:
United States
Language:
English

References (6)

Single-molecule PCR using water-in-oil emulsion journal April 2003
Selective Encapsulation of Single Cells and Subcellular Organelles into Picoliter- and Femtoliter-Volume Droplets journal March 2005
Miniemulsion Droplets as Single Molecule Nanoreactors for Polymerase Chain Reaction journal June 2005
Amplification of complex gene libraries by emulsion PCR journal July 2006
BEAMing: single-molecule PCR on microparticles in water-in-oil emulsions journal July 2006
APPLIED PHYSICS: Droplet Control for Microfluidics journal August 2005

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