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Title: Genome Sequence of the Historical Clinical Isolate Burkholderia pseudomallei PHLS 6

Abstract

We present the draft genome sequence ofBurkholderia pseudomalleiPHLS 6, a virulent clinical strain isolated from a melioidosis patient in Bangladesh in 1960. This draft genome consists of 39 contigs and is 7,322,181 bp long.

Authors:
ORCiD logo [1]; ORCiD logo [2];  [2];  [2];  [3];  [3];  [3];  [3];  [1];  [3];  [1]
  1. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
  2. Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
  3. Sandia National Lab. (SNL-CA), Livermore, CA (United States)
Publication Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
USDOE National Nuclear Security Administration (NNSA)
OSTI Identifier:
1303160
Report Number(s):
SAND2016-4716J
Journal ID: ISSN 2169-8287; 640460
Grant/Contract Number:
AC04-94AL85000
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Genome Announcements
Additional Journal Information:
Journal Volume: 4; Journal Issue: 3; Journal ID: ISSN 2169-8287
Publisher:
American Society for Microbiology
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

D’haeseleer, Patrik, Johnson, Shannon L., Davenport, Karen W., Chain, Patrick S., Schoeniger, Joe, Ray, Debjit, Sinha, Anupama, Williams, Kelly P., Peña, José, Branda, Steven S., and El-Etr, Sahar. Genome Sequence of the Historical Clinical Isolate Burkholderia pseudomallei PHLS 6. United States: N. p., 2016. Web. doi:10.1128/genomeA.00649-16.
D’haeseleer, Patrik, Johnson, Shannon L., Davenport, Karen W., Chain, Patrick S., Schoeniger, Joe, Ray, Debjit, Sinha, Anupama, Williams, Kelly P., Peña, José, Branda, Steven S., & El-Etr, Sahar. Genome Sequence of the Historical Clinical Isolate Burkholderia pseudomallei PHLS 6. United States. doi:10.1128/genomeA.00649-16.
D’haeseleer, Patrik, Johnson, Shannon L., Davenport, Karen W., Chain, Patrick S., Schoeniger, Joe, Ray, Debjit, Sinha, Anupama, Williams, Kelly P., Peña, José, Branda, Steven S., and El-Etr, Sahar. 2016. "Genome Sequence of the Historical Clinical Isolate Burkholderia pseudomallei PHLS 6". United States. doi:10.1128/genomeA.00649-16. https://www.osti.gov/servlets/purl/1303160.
@article{osti_1303160,
title = {Genome Sequence of the Historical Clinical Isolate Burkholderia pseudomallei PHLS 6},
author = {D’haeseleer, Patrik and Johnson, Shannon L. and Davenport, Karen W. and Chain, Patrick S. and Schoeniger, Joe and Ray, Debjit and Sinha, Anupama and Williams, Kelly P. and Peña, José and Branda, Steven S. and El-Etr, Sahar},
abstractNote = {We present the draft genome sequence ofBurkholderia pseudomalleiPHLS 6, a virulent clinical strain isolated from a melioidosis patient in Bangladesh in 1960. This draft genome consists of 39 contigs and is 7,322,181 bp long.},
doi = {10.1128/genomeA.00649-16},
journal = {Genome Announcements},
number = 3,
volume = 4,
place = {United States},
year = 2016,
month = 6
}

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  • Infection by the Gram-negative pathogen Burkholderia pseudomallei results in the disease melioidosis, acquired from the environment in parts of southeast Asia and northern Australia. Clinical symptoms of melioidosis range from acute (fever, pneumonia, septicemia, and localized infection) to chronic (abscesses in various organs and tissues, most commonly occurring in the lungs, liver, spleen, kidney, prostate and skeletal muscle), and persistent infections in humans are difficult to cure. Understanding the basic biology and genomics of B. pseudomallei is imperative for the development of new vaccines and therapeutic interventions. This formidable task is becoming more tractable due to the increasing number ofmore » B. pseudomallei genomes that are being sequenced and compared. Here, we compared three B. pseudomallei genomes, from strains MSHR668, K96243 and 1106a, to identify features that might explain why MSHR668 is more virulent than K96243 and 1106a in a mouse model of B. pseudomallei infection. Our analyses focused on metabolic, virulence and regulatory genes that were present in MSHR668 but absent from both K96243 and 1106a. We also noted features present in K96243 and 1106a but absent from MSHR668, and identified genomic differences that may contribute to variations in virulence noted among the three B. pseudomallei isolates. While this work contributes to our understanding of B. pseudomallei genomics, more detailed experiments are necessary to characterize the relevance of specific genomic features to B. pseudomallei metabolism and virulence. Functional analyses of metabolic networks, virulence and regulation shows promise for examining the effects of B. pseudomallei on host cell metabolism and will lay a foundation for future prediction of the virulence of emerging strains. Continued emphasis in this area will be critical for protection against melioidosis, as a better understanding of what constitutes a fully virulent Burkholderia isolate may provide for better diagnostic and medical countermeasure strategies.« less
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