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Title: Summers_FinalReport_ER64408

Abstract

Elemental mercury, Hg(0) is a contaminant at many DOE sites, especially at Oak Ridge National Laboratory (ORNL) where the spread of spilled Hg and its effects on microbial populations have been monitored for decades. To explore the microbial interactions with Hg, we devised a global proteomic approach capable of directly detecting Hg-adducts of proteins. This technique developed in the facultative anaerobe, Escherichia coli, allows us to identify the proteins most vulnerable to acute exposure to organomercurials phenyl- and ethyl-mercury (as surrogates for the highly neurotoxic methyl-Hg) (Polacco, et al, 2011). We have found >300 such proteins in all metabolic functional groups and cellular compartments; most are highly conserved and can serve as markers for acute Hg exposure (Zink, et al. 2016, in preparation).

Authors:
 [1];  [2];  [3]
  1. Univ. of Georgia, Athens, GA (United States)
  2. Genentech, San Francisco, CA (United States)
  3. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Publication Date:
Research Org.:
Univ. of Georgia, Athens, GA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1302637
Report Number(s):
DOE-UGA-10-21-RR182-348
DOE Contract Number:
FG02-07ER64408
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; mercury; proteomics; mass spectrometry; bacteria

Citation Formats

Summers, Anne, Miller, Susan M., and Lipton, Mary. Summers_FinalReport_ER64408. United States: N. p., 2016. Web. doi:10.2172/1302637.
Summers, Anne, Miller, Susan M., & Lipton, Mary. Summers_FinalReport_ER64408. United States. doi:10.2172/1302637.
Summers, Anne, Miller, Susan M., and Lipton, Mary. Mon . "Summers_FinalReport_ER64408". United States. doi:10.2172/1302637. https://www.osti.gov/servlets/purl/1302637.
@article{osti_1302637,
title = {Summers_FinalReport_ER64408},
author = {Summers, Anne and Miller, Susan M. and Lipton, Mary},
abstractNote = {Elemental mercury, Hg(0) is a contaminant at many DOE sites, especially at Oak Ridge National Laboratory (ORNL) where the spread of spilled Hg and its effects on microbial populations have been monitored for decades. To explore the microbial interactions with Hg, we devised a global proteomic approach capable of directly detecting Hg-adducts of proteins. This technique developed in the facultative anaerobe, Escherichia coli, allows us to identify the proteins most vulnerable to acute exposure to organomercurials phenyl- and ethyl-mercury (as surrogates for the highly neurotoxic methyl-Hg) (Polacco, et al, 2011). We have found >300 such proteins in all metabolic functional groups and cellular compartments; most are highly conserved and can serve as markers for acute Hg exposure (Zink, et al. 2016, in preparation).},
doi = {10.2172/1302637},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Mon Aug 22 00:00:00 EDT 2016},
month = {Mon Aug 22 00:00:00 EDT 2016}
}

Technical Report:

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