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Title: Genomics-informed isolation and characterization of a symbiotic Nanoarchaeota system from a terrestrial geothermal environment

Abstract

Biological features can be inferred, based on genomic data, for many microbial lineages that remain uncultured. However, cultivation is important for characterizing an organism’s physiology and testing its genome-encoded potential. Here we use single-cell genomics to infer cultivation conditions for the isolation of an ectosymbiotic Nanoarchaeota (‘Nanopusillus acidilobi’) and its host (Acidilobus, a crenarchaeote) from a terrestrial geothermal environment. The cells of ‘Nanopusillus’ are among the smallest known cellular organisms (100–300 nm). They appear to have a complete genetic information processing machinery, but lack almost all primary biosynthetic functions as well as respiration and ATP synthesis. Lastly, genomic and proteomic comparison with its distant relative, the marine Nanoarchaeum equitans illustrate an ancient, common evolutionary history of adaptation of the Nanoarchaeota to ectosymbiosis, so far unique among the Archaea.

Authors:
 [1];  [2];  [1];  [1];  [2];  [1];  [3];  [1]
  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States). Dept. of Microbiology
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  3. Portland State Univ., Portland, OR (United States). Dept. of Biology
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
Work for Others (WFO); USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1295113
Grant/Contract Number:  
AC05-00OR22725; SC0006654; DEB1134877
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Nature Communications
Additional Journal Information:
Journal Volume: 7; Journal ID: ISSN 2041-1723
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
Nanopusillus acidilobi; single-cell genomics

Citation Formats

Wurch, Louie, Giannone, Richard J., Belisle, Bernard S., Swift, Carolyn, Utturkar, Sagar, Hettich, Robert L., Reysenbach, Anna-Louise, and Podar, Mircea. Genomics-informed isolation and characterization of a symbiotic Nanoarchaeota system from a terrestrial geothermal environment. United States: N. p., 2016. Web. doi:10.1038/ncomms12115.
Wurch, Louie, Giannone, Richard J., Belisle, Bernard S., Swift, Carolyn, Utturkar, Sagar, Hettich, Robert L., Reysenbach, Anna-Louise, & Podar, Mircea. Genomics-informed isolation and characterization of a symbiotic Nanoarchaeota system from a terrestrial geothermal environment. United States. doi:10.1038/ncomms12115.
Wurch, Louie, Giannone, Richard J., Belisle, Bernard S., Swift, Carolyn, Utturkar, Sagar, Hettich, Robert L., Reysenbach, Anna-Louise, and Podar, Mircea. Tue . "Genomics-informed isolation and characterization of a symbiotic Nanoarchaeota system from a terrestrial geothermal environment". United States. doi:10.1038/ncomms12115. https://www.osti.gov/servlets/purl/1295113.
@article{osti_1295113,
title = {Genomics-informed isolation and characterization of a symbiotic Nanoarchaeota system from a terrestrial geothermal environment},
author = {Wurch, Louie and Giannone, Richard J. and Belisle, Bernard S. and Swift, Carolyn and Utturkar, Sagar and Hettich, Robert L. and Reysenbach, Anna-Louise and Podar, Mircea},
abstractNote = {Biological features can be inferred, based on genomic data, for many microbial lineages that remain uncultured. However, cultivation is important for characterizing an organism’s physiology and testing its genome-encoded potential. Here we use single-cell genomics to infer cultivation conditions for the isolation of an ectosymbiotic Nanoarchaeota (‘Nanopusillus acidilobi’) and its host (Acidilobus, a crenarchaeote) from a terrestrial geothermal environment. The cells of ‘Nanopusillus’ are among the smallest known cellular organisms (100–300 nm). They appear to have a complete genetic information processing machinery, but lack almost all primary biosynthetic functions as well as respiration and ATP synthesis. Lastly, genomic and proteomic comparison with its distant relative, the marine Nanoarchaeum equitans illustrate an ancient, common evolutionary history of adaptation of the Nanoarchaeota to ectosymbiosis, so far unique among the Archaea.},
doi = {10.1038/ncomms12115},
journal = {Nature Communications},
issn = {2041-1723},
number = ,
volume = 7,
place = {United States},
year = {2016},
month = {7}
}

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Cited by: 16 works
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