Simultaneous knockdown of six non-family genes using a single synthetic RNAi fragment in Arabidopsis thaliana

Czarnecki, Olaf; Bryan, Anthony C.; Jawdy, Sara S.; Yang, Xiaohan; Cheng, Zong-Ming; Chen, Jin-Gui; Tuskan, Gerald A.

doi:10.1186/s13007-016-0116-8

Title: Simultaneous knockdown of six non-family genes using a single synthetic RNAi fragment in Arabidopsis thaliana

Abstract

Genetic engineering of plants that results in successful establishment of new biochemical or regulatory pathways requires stable introduction of one or more genes into the plant genome. It might also be necessary to down-regulate or turn off expression of endogenous genes in order to reduce activity of competing pathways. An established way to knockdown gene expression in plants is expressing a hairpin-(hp)-RNAi construct, eventually leading to degradation of a specifically targeted mRNA. Ultimately, knockdown of multiple genes that do not share homologous sequences is still challenging and involves either sophisticated cloning strategies to create vectors with different serial expression constructs or multiple transformation events that is often restricted by a lack of available transformation markers.

Authors:: Czarnecki, Olaf; Bryan, Anthony C.; Jawdy, Sara S.; Yang, Xiaohan; Cheng, Zong-Ming; Chen, Jin-Gui; Tuskan, Gerald A.

Publication Date:: Wed Feb 17 00:00:00 EST 2016

Research Org.:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Org.:: USDOE Office of Science (SC)

OSTI Identifier:: 1618904

Alternate Identifier(s):: OSTI ID: 1294077; OSTI ID: 1311232

Grant/Contract Number:: SC0008834; AC05-00OR22725

Resource Type:: Journal Article: Published Article

Journal Name:: Plant Methods

Additional Journal Information:: Journal Name: Plant Methods Journal Volume: 12 Journal Issue: 1; Journal ID: ISSN 1746-4811

Publisher:: Springer Science + Business Media

Country of Publication:: United Kingdom

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; 58 GEOSCIENCES

Citation Formats


                    Czarnecki, Olaf, Bryan, Anthony C., Jawdy, Sara S., Yang, Xiaohan, Cheng, Zong-Ming, Chen, Jin-Gui, and Tuskan, Gerald A. Simultaneous knockdown of six non-family genes using a single synthetic RNAi fragment in Arabidopsis thaliana.  United Kingdom: N. p., 2016. 
        Web.  doi:10.1186/s13007-016-0116-8.

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                    Czarnecki, Olaf, Bryan, Anthony C., Jawdy, Sara S., Yang, Xiaohan, Cheng, Zong-Ming, Chen, Jin-Gui, & Tuskan, Gerald A. Simultaneous knockdown of six non-family genes using a single synthetic RNAi fragment in Arabidopsis thaliana.  United Kingdom.  https://doi.org/10.1186/s13007-016-0116-8

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                    Czarnecki, Olaf, Bryan, Anthony C., Jawdy, Sara S., Yang, Xiaohan, Cheng, Zong-Ming, Chen, Jin-Gui, and Tuskan, Gerald A. 2016.  
        "Simultaneous knockdown of six non-family genes using a single synthetic RNAi fragment in Arabidopsis thaliana".  United Kingdom.  https://doi.org/10.1186/s13007-016-0116-8.

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@article{osti_1618904,

  title        = {Simultaneous knockdown of six non-family genes using a single synthetic RNAi fragment in Arabidopsis thaliana},

  author       = {Czarnecki, Olaf and Bryan, Anthony C. and Jawdy, Sara S. and Yang, Xiaohan and Cheng, Zong-Ming and Chen, Jin-Gui and Tuskan, Gerald A.},

  abstractNote = {Genetic engineering of plants that results in successful establishment of new biochemical or regulatory pathways requires stable introduction of one or more genes into the plant genome. It might also be necessary to down-regulate or turn off expression of endogenous genes in order to reduce activity of competing pathways. An established way to knockdown gene expression in plants is expressing a hairpin-(hp)-RNAi construct, eventually leading to degradation of a specifically targeted mRNA. Ultimately, knockdown of multiple genes that do not share homologous sequences is still challenging and involves either sophisticated cloning strategies to create vectors with different serial expression constructs or multiple transformation events that is often restricted by a lack of available transformation markers.},

  doi          = {10.1186/s13007-016-0116-8},

  url          = {https://www.osti.gov/biblio/1618904},
  journal      = {Plant Methods},

  issn         = {1746-4811},

  number       = 1,

  volume       = 12,

  place        = {United Kingdom},

  year         = {Wed Feb 17 00:00:00 EST 2016},

  month        = {Wed Feb 17 00:00:00 EST 2016}

}

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GENETIC MODIFICATION OF GIBBERELLIC ACID SIGNALING TO PROMOTE CARBON SEQUESTRATION IN TREE ROOTS AND STEMS

Technical Report Busov, Victor

Semidwarfism has been used extensively in row crops and horticulture to promote yield, reduce lodging, and improve harvest index, and it might have similar benefits for trees for short-rotation forestry or energy plantations, reclamation, phytoremediation, or other applications. We studied the effects of the dominant semidwarfism transgenes GA Insensitive (GAI) and Repressor of GAI-Like, which affect gibberellin (GA) action, and the GA catabolic gene, GA 2-oxidase, in nursery beds and in 2-year-old high-density stands of hybrid poplar (Populus tremula - Populus alba). Twenty-nine traits were analyzed, including measures of growth, morphology, and physiology. Endogenous GA levels were modified in mostmore »« less
https://doi.org/10.2172/1067341

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