Insights into plant cell wall structure, architecture, and integrity using glycome profiling of native and AFEXTM -pre-treated biomass

Pattathil, Sivakumar; Hahn, Michael G.; Dale, Bruce E.; Chundawat, Shishir P.  S.

doi:10.1093/jxb/erv107

Title: Insights into plant cell wall structure, architecture, and integrity using glycome profiling of native and AFEX^TM -pre-treated biomass

Journal Article · Thu Apr 23 00:00:00 EDT 2015 · Journal of Experimental Botany

DOI:https://doi.org/10.1093/jxb/erv107· OSTI ID:1293995

Pattathil, Sivakumar ^[1]; Hahn, Michael G. ^[1]; Dale, Bruce E. ^[2]; Chundawat, Shishir P. S. ^[3]

Univ. of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)
Michigan State Univ., East Lansing, MI (United States). DOE Great Lakes Bioenergy Research Center, Dept. of Chemical Engineering
Michigan State Univ., East Lansing, MI (United States). DOE Great Lakes Bioenergy Research Center, Dept. of Chemical Engineering; Rutgers Univ., Piscataway, NJ (United States). Dept. of Chemical and Biochemical Engineering

We report that cell walls, which constitute the bulk of plant biomass, vary considerably in their structure, composition, and architecture. Studies on plant cell walls can be conducted on both native and pre-treated plant biomass samples, allowing an enhanced understanding of these structural and compositional variations. Here glycome profiling was employed to determine the relative abundance of matrix polysaccharides in several phylogenetically distinct native and pre-treated plant biomasses. Eight distinct biomass types belonging to four different subgroups (i.e. monocot grasses, woody dicots, herbaceous dicots, and softwoods) were subjected to various regimes of AFEX™ (ammonia fiber expansion) pre-treatment [AFEX is a trademark of MBI, Lansing (http://www.mbi.org]. This approach allowed detailed analysis of close to 200 cell wall glycan epitopes and their relative extractability using a high-throughput platform. In general, irrespective of the phylogenetic origin, AFEX™ pre-treatment appeared to cause loosening and improved accessibility of various xylan epitope subclasses in most plant biomass materials studied. For most biomass types analysed, such loosening was also evident for other major non-cellulosic components including subclasses of pectin and xyloglucan epitopes. The studies also demonstrate that AFEX™ pre-treatment significantly reduced cell wall recalcitrance among diverse phylogenies (except softwoods) by inducing structural modifications to polysaccharides that were not detectable by conventional gross composition analyses. Lastly, we found that monitoring changes in cell wall glycan compositions and their relative extractability for untreated and pre-treated plant biomass can provide an improved understanding of variations in structure and composition of plant cell walls and delineate the role(s) of matrix polysaccharides in cell wall recalcitrance.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-00OR22725; FC02-07ER64494; DBI-0421683; IOS-0923992

OSTI ID:: 1293995

Journal Information:: Journal of Experimental Botany, Vol. 66, Issue 14; ISSN 0022-0957

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 42 works

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59 BASIC BIOLOGICAL SCIENCES
09 BIOMASS FUELS
recalcitrance
plant biomass
glycome profiling
AFEX
biofuels
cell walls

Title: Insights into plant cell wall structure, architecture, and integrity using glycome profiling of native and AFEXTM -pre-treated biomass

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References (55)

Cited By (14)

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Title: Insights into plant cell wall structure, architecture, and integrity using glycome profiling of native and AFEX^TM -pre-treated biomass