The composition and organization of Drosophila heterochromatin are heterogeneous and dynamic

Swenson, Joel M.; Colmenares, Serafin U.; Strom, Amy R.; Costes, Sylvain V.; Karpen, Gary H.

doi:10.7554/eLife.16096

Title: The composition and organization of Drosophila heterochromatin are heterogeneous and dynamic

Journal Article · Thu Aug 11 00:00:00 EDT 2016 · eLife

DOI:https://doi.org/10.7554/eLife.16096· OSTI ID:1290303

Swenson, Joel M. ^[1];

^[1]; Strom, Amy R. ^[2]; Costes, Sylvain V. ^[1];

^[2]

Division of Biological Systems and Engineering, Lawrence Berkeley National Laboratory, Berkeley, United States
Division of Biological Systems and Engineering, Lawrence Berkeley National Laboratory, Berkeley, United States, Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States

Heterochromatin is enriched for specific epigenetic factors including Heterochromatin Protein 1a (HP1a), and is essential for many organismal functions. To elucidate heterochromatin organization and regulation, we purified Drosophila melanogaster HP1a interactors, and performed a genome-wide RNAi screen to identify genes that impact HP1a levels or localization. The majority of the over four hundred putative HP1a interactors and regulators identified were previously unknown. We found that 13 of 16 tested candidates (83%) are required for gene silencing, providing a substantial increase in the number of identified components that impact heterochromatin properties. Surprisingly, image analysis revealed that although some HP1a interactors and regulators are broadly distributed within the heterochromatin domain, most localize to discrete subdomains that display dynamic localization patterns during the cell cycle. We conclude that heterochromatin composition and architecture is more spatially complex and dynamic than previously suggested, and propose that a network of subdomains regulates diverse heterochromatin functions.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE; National Institutes of Health (NIH)

Grant/Contract Number:: LB11015; AC02-05CH11231

OSTI ID:: 1290303

Alternate ID(s):: OSTI ID: 1290306; OSTI ID: 1379563

Journal Information:: eLife, Journal Name: eLife Vol. 5; ISSN 2050-084X

Publisher:: eLife Sciences Publications, Ltd.Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 43 works

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