Elimination of formate production in Clostridium thermocellum

Rydzak, Thomas; Lynd, Lee R.; Guss, Adam M.

doi:10.1007/s10295-015-1644-3

Title: Elimination of formate production in Clostridium thermocellum

Journal Article · Sat Jul 11 00:00:00 EDT 2015 · Journal of Industrial Microbiology and Biotechnology

DOI:https://doi.org/10.1007/s10295-015-1644-3· OSTI ID:1286847

Rydzak, Thomas ^[1]; Lynd, Lee R. ^[2]; Guss, Adam M. ^[1]

Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); ; Dartmouth College, Hanover, NH (United States)

We study the ability of Clostridium thermocellum to rapidly degrade cellulose and ferment resulting hydrolysis products into ethanol makes it a promising platform organism for cellulosic biofuel production via consolidated bioprocessing. Currently, however, ethanol yield are far below theoretical maximum due to branched product pathways that divert carbon and electrons towards formate, H₂, lactate, acetate, and secreted amino acids. To redirect carbon and electron flux away from formate, pyruvate:formate lyase (pfl) and respective PFL-activating enzyme were deleted. Formate production in the resulting Δpfl strain was eliminated and acetate production decreased by 50% on both complex and defined medium. Growth rate of Δpfl decreased by 2.9-fold on defined medium and diauxic growth was observed on complex medium. Supplementation of defined medium with 2 mM formate restored Δpfl growth rate to 80% of the parent strain. Finally, we discuss the role of pfl in metabolic engineering strategies and C₁ metabolism.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1286847

Journal Information:: Journal of Industrial Microbiology and Biotechnology, Vol. 42, Issue 9; ISSN 1367-5435

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 23 works

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Title: Elimination of formate production in Clostridium thermocellum

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