Elevated temperature alters proteomic responses of individual organisms within a biofilm community

Mosier, Annika C.; Li, Zhou; Thomas, Brian C.; Hettich, Robert L.; Pan, Chongle; Banfield, Jillian F.

doi:10.1038/ismej.2014.113

Title: Elevated temperature alters proteomic responses of individual organisms within a biofilm community

Journal Article · Tue Jul 22 00:00:00 EDT 2014 · The ISME Journal

DOI:https://doi.org/10.1038/ismej.2014.113· OSTI ID:1286738

^[1]; Li, Zhou ^[2]; Thomas, Brian C. ^[1]; Hettich, Robert L. ^[3]; Pan, Chongle ^[3]; Banfield, Jillian F. ^[4]

Univ. of California, Berkeley, CA (United States). Dept. of Earth and Planetary Science
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee-Oak Ridge National Lab., Knoxville, TN (United States). Graduate School of Genome Science and Technology
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Univ. of California, Berkeley, CA (United States). Dept. of Earth and Planetary Science; Univ. of California, Berkeley, CA (United States). Dept. of Environmental Science, Policy, and Management

Microbial communities that underpin global biogeochemical cycles will likely be influenced by elevated temperature associated with environmental change. Here, we test an approach to measure how elevated temperature impacts the physiology of individual microbial groups in a community context, using a model microbial-based ecosystem. The study is the first application of tandem mass tag (TMT)-based proteomics to a microbial community. We accurately, precisely and reproducibly quantified thousands of proteins in biofilms growing at 40, 43 and 46 °C. Elevated temperature led to upregulation of proteins involved in amino-acid metabolism at the level of individual organisms and the entire community. Proteins from related organisms differed in their relative abundance and functional responses to temperature. Elevated temperature repressed carbon fixation proteins from two Leptospirillum genotypes, whereas carbon fixation proteins were significantly upregulated at higher temperature by a third member of this genus. Leptospirillum group III bacteria may have been subject to viral stress at elevated temperature, which could lead to greater carbon turnover in the microbial food web through the release of viral lysate. Overall, these findings highlight the utility of proteomics-enabled community-based physiology studies, and provide a methodological framework for possible extension to additional mixed culture and environmental sample analyses.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Oak Ridge Leadership Computing Facility (OLCF); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC05-00OR22725; AC02-05CH11231

OSTI ID:: 1286738

Alternate ID(s):: OSTI ID: 1579330

Journal Information:: The ISME Journal, Vol. 9, Issue 1; ISSN 1751-7362

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 39 works

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