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Title: Investigations into the metabolic diversity of microorganisms as part of microbial diversity

Abstract

DOE funds supported a key portion of the MBL Microbial Diversity (Woods Hole) program across 6 complete summers. The initial 4 years of the funded period were overseen by two co-Directors, Daniel Buckley (Cornell) and Steve Zinder (Cornell), who then completed their term. The final 2 summers were overseen by 2 new co-Directors, Jared R. Leadbetter (Caltech) and Dianne Newman (Caltech). The 6 funded summer iterations of the course included the incorporation of new themes such as single cell approaches applied to natural microbial communities (cell separation and sorting, genome amplification from single cells, and the use of Nano-SIMS to examine assimilation of carbon and nitrogen from isotopically labeled substrates into single cells), genetics and genomics on bacteria freshly isolated during the course of the programs, quantitative systems biology, and modern quantitative light microscopy.

Authors:
 [1]
  1. California Inst. of Technology (CalTech), Pasadena, CA (United States)
Publication Date:
Research Org.:
Marine Biological Laboratory, Woods Hole, MA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1272220
Report Number(s):
DOE-MBL-13361
DOE Contract Number:
FG02-85ER13361
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Leadbetter, Jared. Investigations into the metabolic diversity of microorganisms as part of microbial diversity. United States: N. p., 2016. Web. doi:10.2172/1272220.
Leadbetter, Jared. Investigations into the metabolic diversity of microorganisms as part of microbial diversity. United States. doi:10.2172/1272220.
Leadbetter, Jared. 2016. "Investigations into the metabolic diversity of microorganisms as part of microbial diversity". United States. doi:10.2172/1272220. https://www.osti.gov/servlets/purl/1272220.
@article{osti_1272220,
title = {Investigations into the metabolic diversity of microorganisms as part of microbial diversity},
author = {Leadbetter, Jared},
abstractNote = {DOE funds supported a key portion of the MBL Microbial Diversity (Woods Hole) program across 6 complete summers. The initial 4 years of the funded period were overseen by two co-Directors, Daniel Buckley (Cornell) and Steve Zinder (Cornell), who then completed their term. The final 2 summers were overseen by 2 new co-Directors, Jared R. Leadbetter (Caltech) and Dianne Newman (Caltech). The 6 funded summer iterations of the course included the incorporation of new themes such as single cell approaches applied to natural microbial communities (cell separation and sorting, genome amplification from single cells, and the use of Nano-SIMS to examine assimilation of carbon and nitrogen from isotopically labeled substrates into single cells), genetics and genomics on bacteria freshly isolated during the course of the programs, quantitative systems biology, and modern quantitative light microscopy.},
doi = {10.2172/1272220},
journal = {},
number = ,
volume = ,
place = {United States},
year = 2016,
month = 7
}

Technical Report:

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  • The philosophy of the course described here is to underscore the essence of microbiology which is diversity>: diversity of morphology and cellular development, behavior, and metabolic and physiological functions. Emphasis is on microbes other than those customarily covered in conventional microbiology courses and includes: the archaebacteria, extremophiles, and array of obligate anaerobes, various phototrophs, and those microbes exhibiting complex developmental cycles. Also included are microbes carrying out a variety of transformations of organic and inorganic compounds, as well as those which normally occur in symbiotic association with other microbes or with higher forms of life.
  • The philosophy of the course described here is to underscore the essence of microbiology which is diversity>: diversity of morphology and cellular development, behavior, and metabolic and physiological functions. Emphasis is on microbes other than those customarily covered in conventional microbiology courses and includes: the archaebacteria, extremophiles, and array of obligate anaerobes, various phototrophs, and those microbes exhibiting complex developmental cycles. Also included are microbes carrying out a variety of transformations of organic and inorganic compounds, as well as those which normally occur in symbiotic association with other microbes or with higher forms of life.
  • Experiments at NIPER have demonstrated that oil mobilization by microbial formulations is not merely the result of the effects of the metabolic products from the in situ fermentation of nutrient. A combination of two microorganisms, Bacillus licheniformis, NIPER and a Clostridium species (NIPER 6) was determined to be an effective microbial formulation for the recovery of residual crude oil in porous media. Flask tests with various nutrients and environmental conditions were used to evaluate the growth and metabolite production of NIPER 1 and 6. Several interfacial tension (IFT) measurements were conducted using certain metabolic products from the combined microbial culturesmore » NIPER 1 and NIPER 6. Nonane was used as the oil for these experiments, since crude oil from Delaware-Childers field emulsifies very easily. The IFT of a selected microbial formulation were measured with two different crude oils using brines of varying salinities. Comparisons were made with saline brines containing only the nutrient and with microbial metabolite solutions from which the active cells have been removed by filtration to isolate the specific effects of the microbial cells. Etched-glass micromodel studies showed that the microbial formulation effectively mobilized crude oil trapped after waterflooding. Wettability alteration and unsteady-state relative permeability tests were performed in Berea Sandstone cores. 16 refs., 17 figs., 12 tabs.« less
  • Results are summarized from a series of studies on metabolic pathways ion microorganiosms ion which C/sup 14/, S/sup 35/, and H/sup 3./ were used as tracers. (C.H.)