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Title: Effect of aging on lignin content, composition and enzymatic saccharification in Corymbia hybrids and parental taxa between years 9 and 12

Abstract

Corymbia (a eucalypt) is an important forestry genus and a potential lignocellulosic bioenergy feedstock. The composition of the lignocellulosic cell wall significantly impacts pretreatment efficiency and conversion to biofuel but is variable and changes with age. In this study, we estimated Klason lignin content, composition, and monosaccharide (glucose and xylose) release after enzymatic saccharification of untreated and hydrothermally pretreated biomass from Corymbia parental species Corymbia torelliana (CT), Corymbia citriodora subsp. variegata (spotted gum; CCV), and interspecific F1 hybrids (CT x CCV) at ages 9 and 12 years from planting. Analysis of lignin composition derived from syringyl/guaiacyl monolignols (S/G) found significant differences among taxa, with CT S/G ratios (2.2 and 2.0) being significantly lower than CCV (2.6 and 2.3) or hybrids (2.5 and 2.3) at ages 9 and 12 respectively. In general, enzymatic saccharification yields from untreated biomass were significantly different among taxa, with CT (113 and 75 mg g -1) and hybrids (108 and 81 mg g -1) yielding significantly higher glucose from untreated biomass than CCV (82 and 56 mg g -1) at ages 9 and 12 respectively. Comparison of traits within taxa between ages 9 and 12 found S/G ratios and glucose yields from untreated biomass were significantlymore » lower in CT, CCV and hybrid taxa. As a result, the formation of lignocellulosic cell walls is complex, influenced by genetics and age of material, requiring optimization of rotation age for biofuel production and other industrial processes.« less

Authors:
 [1];  [2]; ORCiD logo [3];  [4];  [5];  [5];  [4];  [5];  [6];  [1]
  1. Univ. of Queensland, Queensland (Australia)
  2. Sage Analytics, Boulder, CO (United States)
  3. Univ. of Sunshine Coast, Queensland (Australia); Agri-Science Queensland, Gympie (Australia)
  4. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); National Renewable Energy Lab. (NREL), Golden, CO (United States)
  5. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Sandia National Lab. (SNL-CA), Livermore, CA (United States)
  6. Univ. of Queensland, Queensland (Australia); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Sandia National Lab. (SNL-CA), Livermore, CA (United States)
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1266697
Report Number(s):
NREL/JA-2700-64939
Journal ID: ISSN 0961-9534
Grant/Contract Number:
AC36-08GO28308; AC02-05CH11231
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Biomass and Bioenergy
Additional Journal Information:
Journal Volume: 93; Journal ID: ISSN 0961-9534
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; Corymbia; saccharification; lignin; S/G; bioproducts; biofuels

Citation Formats

Healey, Adam L., Lupoi, Jason S., Lee, David J., Sykes, Robert W., Guenther, Joel M., Tran, Kim, Decker, Stephen R., Singh, Seema, Simmons, Blake A., and Henry, Robert J. Effect of aging on lignin content, composition and enzymatic saccharification in Corymbia hybrids and parental taxa between years 9 and 12. United States: N. p., 2016. Web. doi:10.1016/j.biombioe.2016.06.016.
Healey, Adam L., Lupoi, Jason S., Lee, David J., Sykes, Robert W., Guenther, Joel M., Tran, Kim, Decker, Stephen R., Singh, Seema, Simmons, Blake A., & Henry, Robert J. Effect of aging on lignin content, composition and enzymatic saccharification in Corymbia hybrids and parental taxa between years 9 and 12. United States. doi:10.1016/j.biombioe.2016.06.016.
Healey, Adam L., Lupoi, Jason S., Lee, David J., Sykes, Robert W., Guenther, Joel M., Tran, Kim, Decker, Stephen R., Singh, Seema, Simmons, Blake A., and Henry, Robert J. 2016. "Effect of aging on lignin content, composition and enzymatic saccharification in Corymbia hybrids and parental taxa between years 9 and 12". United States. doi:10.1016/j.biombioe.2016.06.016. https://www.osti.gov/servlets/purl/1266697.
@article{osti_1266697,
title = {Effect of aging on lignin content, composition and enzymatic saccharification in Corymbia hybrids and parental taxa between years 9 and 12},
author = {Healey, Adam L. and Lupoi, Jason S. and Lee, David J. and Sykes, Robert W. and Guenther, Joel M. and Tran, Kim and Decker, Stephen R. and Singh, Seema and Simmons, Blake A. and Henry, Robert J.},
abstractNote = {Corymbia (a eucalypt) is an important forestry genus and a potential lignocellulosic bioenergy feedstock. The composition of the lignocellulosic cell wall significantly impacts pretreatment efficiency and conversion to biofuel but is variable and changes with age. In this study, we estimated Klason lignin content, composition, and monosaccharide (glucose and xylose) release after enzymatic saccharification of untreated and hydrothermally pretreated biomass from Corymbia parental species Corymbia torelliana (CT), Corymbia citriodora subsp. variegata (spotted gum; CCV), and interspecific F1 hybrids (CT x CCV) at ages 9 and 12 years from planting. Analysis of lignin composition derived from syringyl/guaiacyl monolignols (S/G) found significant differences among taxa, with CT S/G ratios (2.2 and 2.0) being significantly lower than CCV (2.6 and 2.3) or hybrids (2.5 and 2.3) at ages 9 and 12 respectively. In general, enzymatic saccharification yields from untreated biomass were significantly different among taxa, with CT (113 and 75 mg g-1) and hybrids (108 and 81 mg g-1) yielding significantly higher glucose from untreated biomass than CCV (82 and 56 mg g-1) at ages 9 and 12 respectively. Comparison of traits within taxa between ages 9 and 12 found S/G ratios and glucose yields from untreated biomass were significantly lower in CT, CCV and hybrid taxa. As a result, the formation of lignocellulosic cell walls is complex, influenced by genetics and age of material, requiring optimization of rotation age for biofuel production and other industrial processes.},
doi = {10.1016/j.biombioe.2016.06.016},
journal = {Biomass and Bioenergy},
number = ,
volume = 93,
place = {United States},
year = 2016,
month = 7
}

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  • In order for a lignocellulosic bioenergy feedstock to be considered sustainable,it must possess a high rate of growth to supply biomass for conversion. Despite the desirability of a fast growth rate for industrial application,it is unclear what effect growth rate has on biomass composition or saccharification. We characterized Klason lignin,glucan,and xylan content with response to growth in Corymbia interspecific F1 hybrid families (HF) and parental species Corymbia torelliana and C. citriodora subspecies variegata and measured the effects on enzymatic hydrolysis from hydrothermally pretreated biomass. Analysis of biomass composition within Corymbia populations found similar amounts of Klason lignin content (19.7–21.3%) amongmore » parental and hybrid populations,whereas glucan content was clearly distinguished within C. citriodora subspecies variegata (52%) and HF148 (60%) as compared to other populations (28–38%). Multiple linear regression indicates that biomass composition is significantly impacted by tree size measured at the same age,with Klason lignin content increasing with diameter breast height (DBH) (+0.12% per cm DBH increase),and glucan and xylan typically decreasing per DBH cm increase (-0.7 and -0.3%,respectively). Polysaccharide content within C. citriodora subspecies variegata and HF-148 were not significantly affected by tree size. High-throughput enzymatic saccharification of hydrothermally pretreated biomass found significant differences among Corymbia populations for total glucose production from biomass,with parental Corymbia torelliana and hybrids HF-148 and HF-51 generating the highest amounts of glucose (~180 mg/g biomass,respectively),with HF-51 undergoing the most efficient glucan-to-glucose conversion (74%). Based on growth rate,biomass composition,and further optimization of enzymatic saccharification yield,high production Corymbia hybrid trees are potentially suitable for fast-rotation bioenergy or biomaterial production.« less
  • A series of correlations was made between the performance of 15 wood species in simultaneous saccharification and fermentation (SSF) and their respective chemical compositions. A compelling inverse trend (P < 0.001) was demonstrated between the percent conversion of glucan to ethanol during SSF and the Mason lignin content of the wood samples before dilute acid pretreatment. No significant relationships were found between the glucan, xylan, and ash compositions of the native wood samples and ethanol yield. This observation is unique and provides a convenient predictor of biomass conversion efficiency. 8 refs., 2 figs., 2 tabs.
  • A series of correlations was made between the performance of 15 wood species in simultaneous saccharification and fermentation (SSF) and their respective chemical compositions. A compelling inverse trend (p<0.001) was demonstrated between the percent conversion of glucan to ethanol during SSF and the Klason lignin content of the wood samples before dilute acid pretreatment. No significant relationships were found between the glucan, xylan, and ash compositons of the native wood samples and ethanol yield. This observation is unique and provides a convenient predictor of biomass conversion efficiency.