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Title: Unraveling the mystery of natural rubber biosynthesis. Part II. Composition and growth of in vitro natural rubber using high-resolution size exclusion chromatography

Abstract

The superior properties of natural rubber (cis-1,4-polyisoprene [NR]) are a function of its structure and composition, properties that still remain a mystery and that are irreplaceable by any synthetic rubber. NR from guayule (Parthenium argentatum) has been gaining special interest for its hypoallergenic properties while maintaining superior mechanical properties that are commonly associated with the Brazilian rubber tree (Hevea brasiliensis), the most common source of NR. Techniques exist to isolate washed rubber particles (WRPs) that contain enzymatically active rubber transferase, to study NR biosynthesis, and previous work on the in vitroNRgrowth in Hevea has demonstrated the presence of around 50wt%of a low molecular weight ([MW], Mn <10 000 g/mol) fraction. Structural and compositional analyses of this low MW fraction in Hevea are challenging due to the high protein content. Here, we discuss the analysis and composition of guayule latex and WRPs using high-resolution Size Exclusion Chromatography. We also discuss the composition of the soluble fraction of inactive guayule latex using matrix-assisted laser desorption ionization/time of flight mass spectrometry.

Authors:
 [1];  [2];  [1];  [3];  [3];  [4];  [4]
  1. Univ. of Akron, OH (United States)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  3. The Ohio State Univ., Wooster, OH (United States)
  4. Univ. of Bordeaux, Pessac Cedex (France)
Publication Date:
Research Org.:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Sciences (CNMS)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1265890
DOE Contract Number:  
AC05-00OR22725
Resource Type:
Journal Article
Journal Name:
Rubber Chemistry and Technology
Additional Journal Information:
Journal Volume: 87; Journal Issue: 3; Journal ID: ISSN 0035-9475
Publisher:
American Chemical Society (ACS), Rubber Division
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE

Citation Formats

Chiang, Cheng Ching K., Barkakaty, Balaka, Puskas, Judit E., Xie, Wenshuang, Cornish, Katrina, Peruch, Federic, and Deffieux, Alain. Unraveling the mystery of natural rubber biosynthesis. Part II. Composition and growth of in vitro natural rubber using high-resolution size exclusion chromatography. United States: N. p., 2014. Web. doi:10.5254/rct.14.87913.
Chiang, Cheng Ching K., Barkakaty, Balaka, Puskas, Judit E., Xie, Wenshuang, Cornish, Katrina, Peruch, Federic, & Deffieux, Alain. Unraveling the mystery of natural rubber biosynthesis. Part II. Composition and growth of in vitro natural rubber using high-resolution size exclusion chromatography. United States. doi:10.5254/rct.14.87913.
Chiang, Cheng Ching K., Barkakaty, Balaka, Puskas, Judit E., Xie, Wenshuang, Cornish, Katrina, Peruch, Federic, and Deffieux, Alain. Mon . "Unraveling the mystery of natural rubber biosynthesis. Part II. Composition and growth of in vitro natural rubber using high-resolution size exclusion chromatography". United States. doi:10.5254/rct.14.87913.
@article{osti_1265890,
title = {Unraveling the mystery of natural rubber biosynthesis. Part II. Composition and growth of in vitro natural rubber using high-resolution size exclusion chromatography},
author = {Chiang, Cheng Ching K. and Barkakaty, Balaka and Puskas, Judit E. and Xie, Wenshuang and Cornish, Katrina and Peruch, Federic and Deffieux, Alain},
abstractNote = {The superior properties of natural rubber (cis-1,4-polyisoprene [NR]) are a function of its structure and composition, properties that still remain a mystery and that are irreplaceable by any synthetic rubber. NR from guayule (Parthenium argentatum) has been gaining special interest for its hypoallergenic properties while maintaining superior mechanical properties that are commonly associated with the Brazilian rubber tree (Hevea brasiliensis), the most common source of NR. Techniques exist to isolate washed rubber particles (WRPs) that contain enzymatically active rubber transferase, to study NR biosynthesis, and previous work on the in vitroNRgrowth in Hevea has demonstrated the presence of around 50wt%of a low molecular weight ([MW], Mn <10 000 g/mol) fraction. Structural and compositional analyses of this low MW fraction in Hevea are challenging due to the high protein content. Here, we discuss the analysis and composition of guayule latex and WRPs using high-resolution Size Exclusion Chromatography. We also discuss the composition of the soluble fraction of inactive guayule latex using matrix-assisted laser desorption ionization/time of flight mass spectrometry.},
doi = {10.5254/rct.14.87913},
journal = {Rubber Chemistry and Technology},
issn = {0035-9475},
number = 3,
volume = 87,
place = {United States},
year = {2014},
month = {9}
}