Changes in Cell Wall Properties Coincide with Overexpression of Extensin Fusion Proteins in Suspension Cultured Tobacco Cells

Tan, Li; Pu, Yunqiao; Pattathil, Sivakumar; Avci, Utku; Qian, Jin; Arter, Allison; Chen, Liwei; Hahn, Michael G.; Ragauskas, Arthur J.; Kieliszewski, Marcia J.

doi:10.1371/journal.pone.0115906

Title: Changes in Cell Wall Properties Coincide with Overexpression of Extensin Fusion Proteins in Suspension Cultured Tobacco Cells

Journal Article · Tue Dec 23 00:00:00 EST 2014 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0115906· OSTI ID:1260605

Tan, Li ^[1]; Pu, Yunqiao ^[2]; Pattathil, Sivakumar ^[1]; Avci, Utku ^[1]; Qian, Jin ^[1]; Arter, Allison ^[3]; Chen, Liwei ^[4]; Hahn, Michael G. ^[1]; Ragauskas, Arthur J. ^[2]; Kieliszewski, Marcia J. ^[3]

Univ. of Georgia, Athens, GA (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division
Ohio Univ., Athens, OH (United States)
Ohio Univ., Athens, OH (United States); Suzhou Inst. of Nano-Tech and Nano-Bionics (SINANO), Suzhou (China)

Extensins are one subfamily of the cell wall hydroxyproline-rich glycoproteins, containing characteristic SerHyp₄ glycosylation motifs and intermolecular cross-linking motifs such as the TyrXaaTyr sequence. Extensins are believed to form a cross-linked network in the plant cell wall through the tyrosine-derivatives isodityrosine, pulcherosine, and di-isodityrosine. Overexpression of three synthetic genes encoding different elastin-arabinogalactan protein-extensin hybrids in tobacco suspension cultured cells yielded novel cross-linking glycoproteins that shared features of the extensins, arabinogalactan proteins and elastin. The cell wall properties of the three transgenic cell lines were all changed, but in different ways. One transgenic cell line showed decreased cellulose crystallinity and increased wall xyloglucan content; the second transgenic cell line contained dramatically increased hydration capacity and notably increased cell wall biomass, increased di-isodityrosine, and increased protein content; the third transgenic cell line displayed wall phenotypes similar to wild type cells, except changed xyloglucan epitope extractability. These data indicate that overexpression of modified extensins may be a route to engineer plants for bioenergy and biomaterial production.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: PS02-06ER6430; MCB- 9874744; 526-HF02; AC05-00OR22725

OSTI ID:: 1260605

Alternate ID(s):: OSTI ID: 1265756

Journal Information:: PLoS ONE, Vol. 9, Issue 12; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 8 works

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Title: Changes in Cell Wall Properties Coincide with Overexpression of Extensin Fusion Proteins in Suspension Cultured Tobacco Cells

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