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Title: Biocompatibility of Soft-Templated Mesoporous Carbons

Abstract

We report that soft-templated mesoporous carbon is morphologically a non-nano type of carbon. It is a relatively newer variety of biomaterial, which has already demonstrated its successful role in drug delivery applications. To investigate the toxicity and biocompatibility, we introduced three types of mesoporous carbons with varying synthesis conditions and pore textural properties. We compared the Brunauer–Emmett–Teller (BET) surface area and pore width and performed cytotoxicity experiments with HeLa cells, cell viability studies with fibroblast cells and hemocomapatibility studies. Cytotoxicity tests reveal that two of the carbons are not cytotoxic, with cell survival over 90%. The mesoporous carbon with the highest surface area showed slight toxicity (~70% cell survival) at the highest carbon concentration of 500 μg/mL. Fibroblast cell viability assays suggested high and constant viability of over 98% after 3 days with no apparent relation with materials property and good visible cell-carbon compatibility. No hemolysis (<1%) was confirmed for all the carbon materials. Protein adsorption experiments with bovine serum albumin (BSA) and fibrinogen revealed a lower protein binding capacity of 0.2–0.6 mg/m 2 and 2–4 mg/m 2 for BSA and fibrinogen, respectively, with lower binding associated with an increase in surface area. The results of this study confirm themore » biocompatibility of soft-templated mesoporous carbons.« less

Authors:
 [1];  [2];  [2];  [3];  [3];  [1];  [2]
  1. Michigan Technological Univ., Houghton, MI (United States). Dept. of Chemical Engineering
  2. Widener Univ., Chester, PA (United States). Dept. of Chemical Engineering
  3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Science (CNMS)
Publication Date:
Research Org.:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Sciences (CNMS)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1265392
DOE Contract Number:
AC05-00OR22725
Resource Type:
Journal Article
Resource Relation:
Journal Name: ACS Applied Materials and Interfaces; Journal Volume: 6; Journal Issue: 17
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; biocompatibility; cell viability; cytotoxicity; hemolysis; mesoporous carbon; protein adsorption

Citation Formats

Gencoglu, Maria F., Spurri, Amanda, Franko, Mitchell, Chen, Jihua, Hensley, Dale K., Heldt, Caryn L., and Saha, Dipendu. Biocompatibility of Soft-Templated Mesoporous Carbons. United States: N. p., 2014. Web. doi:10.1021/am503076u.
Gencoglu, Maria F., Spurri, Amanda, Franko, Mitchell, Chen, Jihua, Hensley, Dale K., Heldt, Caryn L., & Saha, Dipendu. Biocompatibility of Soft-Templated Mesoporous Carbons. United States. doi:10.1021/am503076u.
Gencoglu, Maria F., Spurri, Amanda, Franko, Mitchell, Chen, Jihua, Hensley, Dale K., Heldt, Caryn L., and Saha, Dipendu. Thu . "Biocompatibility of Soft-Templated Mesoporous Carbons". United States. doi:10.1021/am503076u.
@article{osti_1265392,
title = {Biocompatibility of Soft-Templated Mesoporous Carbons},
author = {Gencoglu, Maria F. and Spurri, Amanda and Franko, Mitchell and Chen, Jihua and Hensley, Dale K. and Heldt, Caryn L. and Saha, Dipendu},
abstractNote = {We report that soft-templated mesoporous carbon is morphologically a non-nano type of carbon. It is a relatively newer variety of biomaterial, which has already demonstrated its successful role in drug delivery applications. To investigate the toxicity and biocompatibility, we introduced three types of mesoporous carbons with varying synthesis conditions and pore textural properties. We compared the Brunauer–Emmett–Teller (BET) surface area and pore width and performed cytotoxicity experiments with HeLa cells, cell viability studies with fibroblast cells and hemocomapatibility studies. Cytotoxicity tests reveal that two of the carbons are not cytotoxic, with cell survival over 90%. The mesoporous carbon with the highest surface area showed slight toxicity (~70% cell survival) at the highest carbon concentration of 500 μg/mL. Fibroblast cell viability assays suggested high and constant viability of over 98% after 3 days with no apparent relation with materials property and good visible cell-carbon compatibility. No hemolysis (<1%) was confirmed for all the carbon materials. Protein adsorption experiments with bovine serum albumin (BSA) and fibrinogen revealed a lower protein binding capacity of 0.2–0.6 mg/m2 and 2–4 mg/m2 for BSA and fibrinogen, respectively, with lower binding associated with an increase in surface area. The results of this study confirm the biocompatibility of soft-templated mesoporous carbons.},
doi = {10.1021/am503076u},
journal = {ACS Applied Materials and Interfaces},
number = 17,
volume = 6,
place = {United States},
year = {Thu Aug 21 00:00:00 EDT 2014},
month = {Thu Aug 21 00:00:00 EDT 2014}
}