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Title: In vivo quantitative imaging of photoassimilate transport dynamics and allocation in large plants using a commercial positron emission tomography (PET) scanner

Abstract

Although important aspects of whole-plant carbon allocation in crop plants (e.g., to grain) occur late in development when the plants are large, techniques to study carbon transport and allocation processes have not been adapted for large plants. Positron emission tomography (PET), developed for dynamic imaging in medicine, has been applied in plant studies to measure the transport and allocation patterns of carbohydrates, nutrients, and phytohormones labeled with positron-emitting radioisotopes. However, the cost of PET and its limitation to smaller plants has restricted its use in plant biology. Here we describe the adaptation and optimization of a commercial clinical PET scanner to measure transport dynamics and allocation patterns of 11C-photoassimilates in large crops. Based on measurements of a phantom, we optimized instrument settings, including use of 3-D mode and attenuation correction to maximize the accuracy of measurements. To demonstrate the utility of PET, we measured 11C-photoassimilate transport and allocation in Sorghum bicolor, an important staple crop, at vegetative and reproductive stages (40 and 70 days after planting; DAP). The 11C-photoassimilate transport speed did not change over the two developmental stages. However, within a stem, transport speeds were reduced across nodes, likely due to higher 11C-photoassimilate unloading in the nodes. Photosynthesis inmore » leaves and the amount of 11C that was exported to the rest of the plant decreased as plants matured. In young plants, exported 11C was allocated mostly (88 %) to the roots and stem, but in flowering plants (70 DAP) the majority of the exported 11C (64 %) was allocated to the apex. Our results show that commercial PET scanners can be used reliably to measure whole-plant C-allocation in large plants nondestructively including, importantly, allocation to roots in soil. This capability revealed extreme changes in carbon allocation in sorghum plants, as they advanced to maturity. Further, our results suggest that nodes may be important control points for photoassimilate distribution in crops of the family Poaceae. In conclusion, quantifying real-time carbon allocation and photoassimilate transport dynamics, as demonstrated here, will be important for functional genomic studies to unravel the mechanisms controlling phloem transport in large crop plants, which will provide crucial insights for improving yields.« less

Authors:
 [1];  [2];  [3];  [3];  [3];  [4]
  1. Brookhaven National Lab. (BNL), Upton, NY (United States); Purdue Research Foundation, West Lafayette, IN (United States)
  2. Brookhaven National Lab. (BNL), Upton, NY (United States); Five Eleven Pharma Inc, Philadelphia, PA (United States)
  3. Brookhaven National Lab. (BNL), Upton, NY (United States)
  4. Brookhaven National Lab. (BNL), Upton, NY (United States); Univ. of Arkansas at Monticello, Monticello, AR (United States)
Publication Date:
Research Org.:
Brookhaven National Lab. (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1263483
Grant/Contract Number:  
AC02-98CH10886; MO094
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
BMC Plant Biology
Additional Journal Information:
Journal Volume: 15; Journal Issue: 1; Journal ID: ISSN 1471-2229
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION; Carbon allocation; Positron emission tomography (PET); Transport; Imaging; Carbon-11 (11C)

Citation Formats

Karve, Abhijit A., Alexoff, David, Kim, Dohyun, Schueller, Michael J., Ferrieri, Richard A., and Babst, Benjamin A. In vivo quantitative imaging of photoassimilate transport dynamics and allocation in large plants using a commercial positron emission tomography (PET) scanner. United States: N. p., 2015. Web. doi:10.1186/s12870-015-0658-3.
Karve, Abhijit A., Alexoff, David, Kim, Dohyun, Schueller, Michael J., Ferrieri, Richard A., & Babst, Benjamin A. In vivo quantitative imaging of photoassimilate transport dynamics and allocation in large plants using a commercial positron emission tomography (PET) scanner. United States. doi:10.1186/s12870-015-0658-3.
Karve, Abhijit A., Alexoff, David, Kim, Dohyun, Schueller, Michael J., Ferrieri, Richard A., and Babst, Benjamin A. Mon . "In vivo quantitative imaging of photoassimilate transport dynamics and allocation in large plants using a commercial positron emission tomography (PET) scanner". United States. doi:10.1186/s12870-015-0658-3. https://www.osti.gov/servlets/purl/1263483.
@article{osti_1263483,
title = {In vivo quantitative imaging of photoassimilate transport dynamics and allocation in large plants using a commercial positron emission tomography (PET) scanner},
author = {Karve, Abhijit A. and Alexoff, David and Kim, Dohyun and Schueller, Michael J. and Ferrieri, Richard A. and Babst, Benjamin A.},
abstractNote = {Although important aspects of whole-plant carbon allocation in crop plants (e.g., to grain) occur late in development when the plants are large, techniques to study carbon transport and allocation processes have not been adapted for large plants. Positron emission tomography (PET), developed for dynamic imaging in medicine, has been applied in plant studies to measure the transport and allocation patterns of carbohydrates, nutrients, and phytohormones labeled with positron-emitting radioisotopes. However, the cost of PET and its limitation to smaller plants has restricted its use in plant biology. Here we describe the adaptation and optimization of a commercial clinical PET scanner to measure transport dynamics and allocation patterns of 11C-photoassimilates in large crops. Based on measurements of a phantom, we optimized instrument settings, including use of 3-D mode and attenuation correction to maximize the accuracy of measurements. To demonstrate the utility of PET, we measured 11C-photoassimilate transport and allocation in Sorghum bicolor, an important staple crop, at vegetative and reproductive stages (40 and 70 days after planting; DAP). The 11C-photoassimilate transport speed did not change over the two developmental stages. However, within a stem, transport speeds were reduced across nodes, likely due to higher 11C-photoassimilate unloading in the nodes. Photosynthesis in leaves and the amount of 11C that was exported to the rest of the plant decreased as plants matured. In young plants, exported 11C was allocated mostly (88 %) to the roots and stem, but in flowering plants (70 DAP) the majority of the exported 11C (64 %) was allocated to the apex. Our results show that commercial PET scanners can be used reliably to measure whole-plant C-allocation in large plants nondestructively including, importantly, allocation to roots in soil. This capability revealed extreme changes in carbon allocation in sorghum plants, as they advanced to maturity. Further, our results suggest that nodes may be important control points for photoassimilate distribution in crops of the family Poaceae. In conclusion, quantifying real-time carbon allocation and photoassimilate transport dynamics, as demonstrated here, will be important for functional genomic studies to unravel the mechanisms controlling phloem transport in large crop plants, which will provide crucial insights for improving yields.},
doi = {10.1186/s12870-015-0658-3},
journal = {BMC Plant Biology},
issn = {1471-2229},
number = 1,
volume = 15,
place = {United States},
year = {2015},
month = {11}
}

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Works referenced in this record:

From PET detectors to PET scanners
journal, October 2003

  • Humm, John L.; Rosenfeld, Anatoly; Del Guerra, Alberto
  • European Journal of Nuclear Medicine and Molecular Imaging, Vol. 30, Issue 11, p. 1574-1597
  • DOI: 10.1007/s00259-003-1266-2