Characterization of Shiga toxin subtypes and virulence genes in porcine Shiga toxin-producing Escherichia coli

Baranzoni, Gian Marco; Fratamico, Pina M.; Gangiredla, Jayanthi; Patel, Isha; Bagi, Lori K.; Delannoy, Sabine; Fach, Patrick; Boccia, Federica; Anastasio, Aniello; Pepe, Tiziana

doi:10.3389/fmicb.2016.00574

Title: Characterization of Shiga toxin subtypes and virulence genes in porcine Shiga toxin-producing Escherichia coli

Journal Article · Thu Apr 21 00:00:00 EDT 2016 · Frontiers in Microbiology

DOI:https://doi.org/10.3389/fmicb.2016.00574· OSTI ID:1261891

Baranzoni, Gian Marco ^[1]; Fratamico, Pina M. ^[1]; Gangiredla, Jayanthi ^[2]; Patel, Isha ^[2]; Bagi, Lori K. ^[1]; Delannoy, Sabine ^[3]; Fach, Patrick ^[3]; Boccia, Federica ^[4]; Anastasio, Aniello ^[4]; Pepe, Tiziana ^[4]

United States Dept. of Agriculture - Agricultural Research Service, Wyndmoor, PA (United States)
U.S. Food and Drug Administration, Laurel, MD (United States)
Univ. of Paris-Est, Maisons-Alfort (France)
Univ. of Naples Federico II, Naples (Italy)

Similar to ruminants, swine have been shown to be a reservoir for Shiga toxin-producing Escherichia coli (STEC), and pork products have been linked with outbreaks associated with STEC O157 and O111:H-. STEC strains, isolated in a previous study from fecal samples of late-finisher pigs, belonged to a total of 56 serotypes, including O15:H27, O91:H14, and other serogroups previously associated with human illness. The isolates were tested by polymerase chain reaction (PCR) and a high-throughput real-time PCR system to determine the Shiga toxin (Stx) subtype and virulence-associated and putative virulence-associated genes they carried. Select STEC strains were further analyzed using a Minimal Signature E. coli Array Strip. As expected, stx_2e (81%) was the most common Stx variant, followed by stx_1a (14%), stx_2d (3%), and stx_1c (1%). The STEC serogroups that carried stx_2d were O15:H27, O159:H16 and O159:H-. Similar to stx_2a and stx_2c, the stx_2d variant is associated with development of hemorrhagic colitis and hemolytic uremic syndrome, and reports on the presence of this variant in STEC strains isolated from swine are lacking. Moreover, the genes encoding heat stable toxin (estIa) and enteroaggregative E. coli heat stable enterotoxin-1 (astA) were commonly found in 50 and 44% of isolates, respectively. The hemolysin genes, hlyA and ehxA, were both detected in 7% of the swine STEC strains. Although the eae gene was not found, other genes involved in host cell adhesion, including lpfA_O113 and paa were detected in more than 50% of swine STEC strains, and a number of strains also carried iha, lpfA_O26, lpfA_O157, fedA, orfA, and orfB. Furthermore, the present work provides new insights on the distribution of virulence factors among swine STEC strains and shows that swine may carry Stx1a-, Stx2e-, or Stx2d-producing E. coli with virulence gene profiles associated with human infections.

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Research Organization:: Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE; ORAU/ORISE; USDA; (ARS) Research Participation Program; French joint ministerial program of R&D against CBRNE risks

Grant/Contract Number:: AC05-06OR23100

OSTI ID:: 1261891

Journal Information:: Frontiers in Microbiology, Vol. 7; ISSN 1664-302X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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Toxins of locus of enterocyte effacement-negative shiga toxin-producing escherichia coli Krause, Maike; Barth, Holger; Schmidt, Herbert Universität Ulm https://doi.org/10.18725/oparu-42860	text	January 2018
A Topographical Atlas of Shiga Toxin 2e Receptor Distribution in the Tissues of Weaned Piglets Steil, Daniel; Bonse, Robert; Meisen, Iris Toxins, Vol. 8, Issue 12 https://doi.org/10.3390/toxins8120357	journal	November 2016
Shiga toxin-producing Escherichia coli in slaughtered pigs and pork products Bardasi, Lia; Taddei, Roberta; Fiocchi, Ilaria Italian Journal of Food Safety, Vol. 6, Issue 2 https://doi.org/10.4081/ijfs.2017.6584	journal	May 2017
Prevalence of O-serogroups, virulence genes, and F18 antigenic variants inEscherichia coliisolated from weaned piglets with diarrhea in Korea during 2008–2016 Do, Kyung-Hyo; Byun, Jae-Won; Lee, Wan-Kyu Journal of Veterinary Science, Vol. 20, Issue 1 https://doi.org/10.4142/jvs.2019.20.1.43	journal	January 2019

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non-o157
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