Proteomic analysis of Chlorella vulgaris: Potential targets for enhanced lipid accumulation
Oleaginous microalgae are capable of producing large quantities of fatty acids and triacylglycerides. As such, they are promising feedstocks for the production of biofuels and bioproducts. Genetic strain-engineering strategies offer a means to accelerate the commercialization of algal biofuels by improving the rate and total accumulation of microalgal lipids. However, the industrial potential of these organisms remains to be met, largely due to the incomplete knowledgebase surrounding the mechanisms governing the induction of algal lipid biosynthesis. Such strategies require further elucidation of genes and gene products controlling algal lipid accumulation. In this study, we have set out to examine these mechanisms and identify novel strain-engineering targets in the oleaginous microalga, Chlorella vulgaris. Comparative shotgun proteomic analyses have identified a number of novel targets, including previously unidentified transcription factors and proteins involved in cell signaling and cell cycle regulation. These results lay the foundation for strain-improvement strategies and demonstrate the power of translational proteomic analysis.
- Research Organization:
- National Renewable Energy Lab. (NREL), Golden, CO (United States)
- Sponsoring Organization:
- USDOE. Office of Energy Efficiency and Renewable Energy Biomass Program
- DOE Contract Number:
- AC36-08GO28308
- OSTI ID:
- 1260919
- Report Number(s):
- NREL/JA-5100-58983
- Journal Information:
- Journal of Proteomics, Vol. 93, Issue C; ISSN 1874-3919
- Country of Publication:
- United States
- Language:
- English
Similar Records
Optimized inorganic carbon regime for enhanced growth and lipid accumulation in Chlorella vulgaris
Chapter 5: Methods for Algal Protein Isolation and Proteome Analysis