skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Characterization and quantification in capillary zone electrophoresis using direct and indirect detection

Abstract

Capillary zone electrophoresis has become a major separation technique for the analysis of small volumes of materials and it is featured with the advantages of high efficiency, simplicity and rapidity. The purpose of this study was to characterize the performance of a capillary electrophoresis system in regards to the separation efficiency and quantitative capacity. Three sample introduction techniques--electromigration, gravity and pressure injection modes--were evaluated utilizing two different sample-buffer systems. Analytes phenol, benzoic acid and 2,4-dihydroxybenzoic acid were separated underpositive polarity voltage using a borate buffet and monitored with direct UV absorption detection. Mixtures of myo-inositol 2-monophosphate, myo-inositol 1,4,5-trisphosphate, and myo-inositol hexakisphosphate were separated under negative polarity voltage using a lon Phor Anionic Electrolyte Buffer containing an electroosmotic flow modifier and a background chromophore for indirect detection.

Authors:
;  [1]
  1. East Carolina Univ., Greenville, NC (United States)
Publication Date:
OSTI Identifier:
126090
Report Number(s):
CONF-950402-
TRN: 95:006086-0059
Resource Type:
Conference
Resource Relation:
Conference: 209. American Chemical Society (ACS) national meeting, Anaheim, CA (United States), 2-6 Apr 1995; Other Information: PBD: 1995; Related Information: Is Part Of 209th ACS national meeting; PB: 2088 p.
Country of Publication:
United States
Language:
English
Subject:
40 CHEMISTRY; ELECTROPHORESIS; EFFICIENCY; DETECTION; BENZOIC ACID; MIXTURES; PHENOL; PHOSPHATES; SEPARATION PROCESSES; ENVIRONMENTAL MATERIALS

Citation Formats

Chiang, Hui-Ti, and Church, W H. Characterization and quantification in capillary zone electrophoresis using direct and indirect detection. United States: N. p., 1995. Web.
Chiang, Hui-Ti, & Church, W H. Characterization and quantification in capillary zone electrophoresis using direct and indirect detection. United States.
Chiang, Hui-Ti, and Church, W H. Fri . "Characterization and quantification in capillary zone electrophoresis using direct and indirect detection". United States.
@article{osti_126090,
title = {Characterization and quantification in capillary zone electrophoresis using direct and indirect detection},
author = {Chiang, Hui-Ti and Church, W H},
abstractNote = {Capillary zone electrophoresis has become a major separation technique for the analysis of small volumes of materials and it is featured with the advantages of high efficiency, simplicity and rapidity. The purpose of this study was to characterize the performance of a capillary electrophoresis system in regards to the separation efficiency and quantitative capacity. Three sample introduction techniques--electromigration, gravity and pressure injection modes--were evaluated utilizing two different sample-buffer systems. Analytes phenol, benzoic acid and 2,4-dihydroxybenzoic acid were separated underpositive polarity voltage using a borate buffet and monitored with direct UV absorption detection. Mixtures of myo-inositol 2-monophosphate, myo-inositol 1,4,5-trisphosphate, and myo-inositol hexakisphosphate were separated under negative polarity voltage using a lon Phor Anionic Electrolyte Buffer containing an electroosmotic flow modifier and a background chromophore for indirect detection.},
doi = {},
url = {https://www.osti.gov/biblio/126090}, journal = {},
number = ,
volume = ,
place = {United States},
year = {1995},
month = {12}
}

Conference:
Other availability
Please see Document Availability for additional information on obtaining the full-text document. Library patrons may search WorldCat to identify libraries that hold this conference proceeding.

Save / Share: