Speeding up biomolecular interactions by molecular sledding
- Univ. of Groningen (Netherlands)
- Brookhaven National Lab. (BNL), Upton, NY (United States)
In numerous biological processes associations involve a protein with its binding partner, an event that is preceded by a diffusion-mediated search bringing the two partners together. Often hindered by crowding in biologically relevant environments, three-dimensional diffusion can be slow and result in long bimolecular association times. Moreover, the initial association step between two binding partners often represents a rate-limiting step in biotechnologically relevant reactions. We also demonstrate the practical use of an 11-a.a. DNA-interacting peptide derived from adenovirus to reduce the dimensionality of diffusional search processes and speed up associations between biological macromolecules. We functionalize binding partners with the peptide and demonstrate that the ability of the peptide to one-dimensionally diffuse along DNA results in a 20-fold reduction in reaction time. We also show that modifying PCR primers with the peptide sled enables significant acceleration of standard PCR reactions.
- Research Organization:
- Brookhaven National Lab. (BNL), Upton, NY (United States)
- Sponsoring Organization:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- Grant/Contract Number:
- SC00112704
- OSTI ID:
- 1260167
- Report Number(s):
- BNL-112327-2016-JA; CSHCBM
- Journal Information:
- Chemical Science, Vol. 7, Issue 2; ISSN 2041-6520
- Publisher:
- Royal Society of ChemistryCopyright Statement
- Country of Publication:
- United States
- Language:
- English
Web of Science
Catalytic transport of molecular cargo using diffusive binding along a polymer track
|
journal | January 2019 |
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