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Title: Fusion proteins useful for producing pinene

Abstract

The present invention provides for a modified host cell comprising a heterologous pinene synthase (PS), or enzymatically active fragment or variant thereof, and optionally a geranyl pyrophosphate synthase (GPPS), or enzymatically active fragment or variant thereof, or a fusion protein comprising: (a) a PS and (b) a GPPS linked by a linker.

Inventors:
;
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1259654
Patent Number(s):
9,376,691
Application Number:
14/091,818
Assignee:
The Regents of the University of California (Oakland, CA) LBNL
DOE Contract Number:
AC02-05CH11231
Resource Type:
Patent
Resource Relation:
Patent File Date: 2013 Nov 27
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Peralta-Yahya, Pamela P., and Keasling, Jay D. Fusion proteins useful for producing pinene. United States: N. p., 2016. Web.
Peralta-Yahya, Pamela P., & Keasling, Jay D. Fusion proteins useful for producing pinene. United States.
Peralta-Yahya, Pamela P., and Keasling, Jay D. Tue . "Fusion proteins useful for producing pinene". United States. doi:. https://www.osti.gov/servlets/purl/1259654.
@article{osti_1259654,
title = {Fusion proteins useful for producing pinene},
author = {Peralta-Yahya, Pamela P. and Keasling, Jay D},
abstractNote = {The present invention provides for a modified host cell comprising a heterologous pinene synthase (PS), or enzymatically active fragment or variant thereof, and optionally a geranyl pyrophosphate synthase (GPPS), or enzymatically active fragment or variant thereof, or a fusion protein comprising: (a) a PS and (b) a GPPS linked by a linker.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jun 28 00:00:00 EDT 2016},
month = {Tue Jun 28 00:00:00 EDT 2016}
}

Patent:

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  • Method and apparatus are disclosed for producing a useful stream of hot gas from a fluidized bed combustor and controlling bed temperature by introducing cooling gas into the freeboard region of the combustor. The cooling gas is introduced by a plurality of inlet ports located within the combustion chamber or about its periphery. The cooling gas contacts particulate matter in the freeboard region, extracting heat therefrom. Cooled particulate matter either falls back into the bed or is returned to the bed from a separator downstream of the combustion chamber.
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  • The invention relates to a host cell comprising at least four different heterologous polynucleotides chosen from the group of polynucleotides encoding cellulases, hemicellulases and pectinases, wherein the host cell is capable of producing the at least four different enzymes chosen from the group of cellulases, hemicellulases and pectinases, wherein the host cell is a filamentous fungus and is capable of secretion of the at least four different enzymes. This host cell can suitably be used for the production of an enzyme composition that can be used in a process for the saccharification of cellulosic material.
  • The invention relates to a host cell comprising at least four different heterologous polynucleotides chosen from the group of polynucleotides encoding cellulases, hemicellulases and pectinases, wherein the host cell is capable of producing the at least four different enzymes chosen from the group of cellulases, hemicellulases and pectinases, wherein the host cell is a filamentous fungus and is capable of secretion of the at least four different enzymes. This host cell can suitably be used for the production of an enzyme composition that can be used in a process for the saccharification of cellulosic material.