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Fusion proteins useful for producing pinene

Patent ·
OSTI ID:1259654
;
The present invention provides for a modified host cell comprising a heterologous pinene synthase (PS), or enzymatically active fragment or variant thereof, and optionally a geranyl pyrophosphate synthase (GPPS), or enzymatically active fragment or variant thereof, or a fusion protein comprising: (a) a PS and (b) a GPPS linked by a linker.
Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC02-05CH11231
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Number(s):
9,376,691
Application Number:
14/091,818
OSTI ID:
1259654
Country of Publication:
United States
Language:
English

References (11)

Microbial production of fatty-acid-derived fuels and chemicals from plant biomass journal January 2010
Monitoring and Analyzing Process Streams Towards Understanding Ionic Liquid Pretreatment of Switchgrass (Panicum virgatum L.) journal April 2010
Proteome-based identification of fusion partner for high-level extracellular production of recombinant proteins in Escherichia coli journal October 2008
Characterization of a Bifunctional Cellulase and Its Structural Gene: THE cel GENE OF BACILLUS SP. D04 HAS EXO- AND ENDOGLUCANASE ACTIVITY journal October 1995
cDNA isolation, functional expression, and characterization of (+)-α-pinene synthase and (−)-α-pinene synthase from loblolly pine (Pinus taeda): Stereocontrol in pinene biosynthesis journal March 2003
Engineering a mevalonate pathway in Escherichia coli for production of terpenoids journal June 2003
Geranyl diphosphate synthase from Abies grandis: cDNA isolation, functional expression, and characterization journal September 2002
Metabolic engineering for advanced biofuels production from Escherichia coli journal October 2008
Insights into Plant Cell Wall Degradation from the Genome Sequence of the Soil Bacterium Cellvibrio japonicus journal June 2008
Characterization of the gene celD and its encoded product 1,4-β-d-glucan glucohydrolase D from Pseudomonas fluorescens subsp. cellulosa journal August 1992
Synthesis of three advanced biofuels from ionic liquid-pretreated switchgrass using engineered Escherichia coli journal November 2011

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09 BIOMASS FUELS
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