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Title: Useful halophilic, thermostable and ionic liquids tolerant cellulases

Abstract

The present invention provides for an isolated or recombinant polypeptide comprising an amino acid sequence having at least 70% identity with the amino acid sequence of a Halorhabdus utahensis cellulase, such as Hu-CBH1, wherein said amino acid sequence has a halophilic thermostable and/or thermophilic cellobiohydrolase (CBH) activity. In some embodiments, the polypeptide has a CBH activity that is resistant to up to about 20% of ionic liquids. The present invention also provides for compositions comprising and methods using the isolated or recombinant polypeptide.

Inventors:
; ; ;
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1259651
Patent Number(s):
9,376,728
Application Number:
13/493,938
Assignee:
The Regents of the University of California (Oakland, CA) LBNL
DOE Contract Number:
AC02-05CH11231
Resource Type:
Patent
Resource Relation:
Patent File Date: 2012 Jun 11
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Zhang, Tao, Datta, Supratim, Simmons, Blake A., and Rubin, Edward M.. Useful halophilic, thermostable and ionic liquids tolerant cellulases. United States: N. p., 2016. Web.
Zhang, Tao, Datta, Supratim, Simmons, Blake A., & Rubin, Edward M.. Useful halophilic, thermostable and ionic liquids tolerant cellulases. United States.
Zhang, Tao, Datta, Supratim, Simmons, Blake A., and Rubin, Edward M.. 2016. "Useful halophilic, thermostable and ionic liquids tolerant cellulases". United States. doi:. https://www.osti.gov/servlets/purl/1259651.
@article{osti_1259651,
title = {Useful halophilic, thermostable and ionic liquids tolerant cellulases},
author = {Zhang, Tao and Datta, Supratim and Simmons, Blake A. and Rubin, Edward M.},
abstractNote = {The present invention provides for an isolated or recombinant polypeptide comprising an amino acid sequence having at least 70% identity with the amino acid sequence of a Halorhabdus utahensis cellulase, such as Hu-CBH1, wherein said amino acid sequence has a halophilic thermostable and/or thermophilic cellobiohydrolase (CBH) activity. In some embodiments, the polypeptide has a CBH activity that is resistant to up to about 20% of ionic liquids. The present invention also provides for compositions comprising and methods using the isolated or recombinant polypeptide.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = 2016,
month = 6
}

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