Genome sequence and description of the anaerobic lignin-degrading bacterium Tolumonas lignolytica sp. nov.

Billings, Andrew F.; Fortney, Julian L.; Hazen, Terry C.; Simmons, Blake; Davenport, Karen W.; Goodwin, Lynne; Ivanova, Natalia; Kyrpides, Nikos C.; Mavromatis, Konstantinos; Woyke, Tanja; DeAngelis, Kristen M.

doi:10.1186/s40793-015-0100-3

Title: Genome sequence and description of the anaerobic lignin-degrading bacterium Tolumonas lignolytica sp. nov.

Journal Article · Thu Nov 19 00:00:00 EST 2015 · Standards in Genomic Sciences

DOI:https://doi.org/10.1186/s40793-015-0100-3· OSTI ID:1259501

Billings, Andrew F. ^[1]; Fortney, Julian L. ^[2]; Hazen, Terry C. ^[3]; Simmons, Blake ^[4]; Davenport, Karen W. ^[5]; Goodwin, Lynne ^[5]; Ivanova, Natalia ^[6]; Kyrpides, Nikos C. ^[6]; Mavromatis, Konstantinos ^[6]; Woyke, Tanja ^[6]; DeAngelis, Kristen M. ^[1]

Univ. of Massachusetts, Amherst, MA (United States)
Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Univ. of Tennessee, Knoxville, TN (United States). Dept. of Civil & Environmental Engineering
Univ. of Tennessee, Knoxville, TN (United States). Dept. of Civil & Environmental Engineering; Univ. of Tennessee, Knoxville, TN (United States). Dept. of Microbiology; Univ. of Tennessee, Knoxville, TN (United States). Dept. of Earth & Planetary Sciences
Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Sandia National Lab. (SNL-CA), Livermore, CA (United States)
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)

Â© 2015 Billings et al. Tolumonas lignolytica BRL6-1 T sp. nov. is the type strain of T. lignolytica sp. nov., a proposed novel species of the Tolumonas genus. This strain was isolated from tropical rainforest soils based on its ability to utilize lignin as a sole carbon source. Cells of Tolumonas lignolytica BRL6-1 T are mesophilic, non-spore forming, Gram-negative rods that are oxidase and catalase negative. The genome for this isolate was sequenced and returned in seven unique contigs totaling 3.6Mbp, enabling the characterization of several putative pathways for lignin breakdown. Particularly, we found an extracellular peroxidase involved in lignin depolymerization, as well as several enzymes involved in β-aryl ether bond cleavage, which is the most abundant linkage between lignin monomers. We also found genes for enzymes involved in ferulic acid metabolism, which is a common product of lignin breakdown. By characterizing pathways and enzymes employed in the bacterial breakdown of lignin in anaerobic environments, this work should assist in the efficient engineering of biofuel production from lignocellulosic material.

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Research Organization:: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1259501

Alternate ID(s):: OSTI ID: 1378652

Journal Information:: Standards in Genomic Sciences, Vol. 10, Issue 1; ISSN 1944-3277

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 39 works

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Genome sequence and description of the anaerobic lignin-degrading bacterium Tolumonas lignolytica sp. nov. [Supplemental Data] Billings, Andrew; Fortney, Julian; Hazen, Terry https://doi.org/10.6084/m9.figshare.c.3630179 The current record is supplemented by this dataset	dataset	November 2015
Genome sequence and description of the anaerobic lignin-degrading bacterium Tolumonas lignolytica sp. nov. [Supplemental Data] Billings, Andrew; Fortney, Julian; Hazen, Terry https://doi.org/10.6084/m9.figshare.c.3630179.v1 The current record is supplemented by this dataset	dataset	November 2015
Additional file 1: Table S1. of Genome sequence and description of the anaerobic lignin-degrading bacterium Tolumonas lignolytica sp. nov. Billings, Andrew; Fortney, Julian; Hazen, Terry https://doi.org/10.6084/m9.figshare.c.3630179_d1 The current record is supplemented by this dataset	dataset	November 2015
Additional file 1: Table S1. of Genome sequence and description of the anaerobic lignin-degrading bacterium Tolumonas lignolytica sp. nov. Billings, Andrew; Fortney, Julian; Hazen, Terry https://doi.org/10.6084/m9.figshare.c.3630179_d1.v1 The current record is supplemented by this dataset	dataset	November 2015