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Title: Rapid discovery and functional characterization of terpene synthases from four endophytic xylariaceae

Abstract

Endophytic fungi are ubiquitous plant endosymbionts that establish complex and poorly understood relationships with their host organisms. Many endophytic fungi are known to produce a wide spectrum of volatile organic compounds (VOCs) with potential energy applications, which have been described as "mycodiesel". Many of these mycodiesel hydrocarbons are terpenes, a chemically diverse class of compounds produced by many plants, fungi, and bacteria. Due to their high energy densities, terpenes, such as pinene and bisabolene, are actively being investigated as potential "drop-in" biofuels for replacing diesel and aviation fuel. In this study, we rapidly discovered and characterized 26 terpene synthases (TPSs) derived from four endophytic fungi known to produce mycodiesel hydrocarbons. The TPS genes were expressed in an E. coli strain harboring a heterologous mevalonate pathway designed to enhance terpene production, and their product profiles were determined using Solid Phase Micro-Extraction (SPME) and GC-MS. Lastly, out of the 26 TPS’s profiled, 12 TPS’s were functional, with the majority of them exhibiting both monoterpene and sesquiterpene synthase activity.

Authors:
 [1];  [1];  [1];  [2];  [2];  [3];  [4]
  1. Sandia National Lab. (SNL-CA), Livermore, CA (United States)
  2. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Joint BioEnergy Institute, Emeryville, CA (United States)
  3. Sandia National Lab. (SNL-CA), Livermore, CA (United States); Joint BioEnergy Institute, Emeryville, CA (United States)
  4. Michigan State Univ., East Lansing, MI (United States)
Publication Date:
Research Org.:
Sandia National Lab. (SNL-CA), Livermore, CA (United States); Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE National Nuclear Security Administration (NNSA); USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1257274
Alternate Identifier(s):
OSTI ID: 1266786; OSTI ID: 1333928; OSTI ID: 1379058
Report Number(s):
SAND-2015-5056J
Journal ID: ISSN 1932-6203
Grant/Contract Number:  
AC04-94AL85000; AC02-05CH11231
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
PLoS ONE
Additional Journal Information:
Journal Volume: 11; Journal Issue: 2; Journal ID: ISSN 1932-6203
Publisher:
Public Library of Science
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; terpenes; sequence motif analysis; fungi; fungal genomics; biosynthesis; cations; cluster compounds; sequence alignment; sequence ailment

Citation Formats

Wu, Weihua, Tran, William, Taatjes, Craig A., Alonso-Gutierrez, Jorge, Lee, Taek Soon, Gladden, John M., and Hamberger, Bjorn. Rapid discovery and functional characterization of terpene synthases from four endophytic xylariaceae. United States: N. p., 2016. Web. doi:10.1371/journal.pone.0146983.
Wu, Weihua, Tran, William, Taatjes, Craig A., Alonso-Gutierrez, Jorge, Lee, Taek Soon, Gladden, John M., & Hamberger, Bjorn. Rapid discovery and functional characterization of terpene synthases from four endophytic xylariaceae. United States. doi:10.1371/journal.pone.0146983.
Wu, Weihua, Tran, William, Taatjes, Craig A., Alonso-Gutierrez, Jorge, Lee, Taek Soon, Gladden, John M., and Hamberger, Bjorn. Wed . "Rapid discovery and functional characterization of terpene synthases from four endophytic xylariaceae". United States. doi:10.1371/journal.pone.0146983. https://www.osti.gov/servlets/purl/1257274.
@article{osti_1257274,
title = {Rapid discovery and functional characterization of terpene synthases from four endophytic xylariaceae},
author = {Wu, Weihua and Tran, William and Taatjes, Craig A. and Alonso-Gutierrez, Jorge and Lee, Taek Soon and Gladden, John M. and Hamberger, Bjorn},
abstractNote = {Endophytic fungi are ubiquitous plant endosymbionts that establish complex and poorly understood relationships with their host organisms. Many endophytic fungi are known to produce a wide spectrum of volatile organic compounds (VOCs) with potential energy applications, which have been described as "mycodiesel". Many of these mycodiesel hydrocarbons are terpenes, a chemically diverse class of compounds produced by many plants, fungi, and bacteria. Due to their high energy densities, terpenes, such as pinene and bisabolene, are actively being investigated as potential "drop-in" biofuels for replacing diesel and aviation fuel. In this study, we rapidly discovered and characterized 26 terpene synthases (TPSs) derived from four endophytic fungi known to produce mycodiesel hydrocarbons. The TPS genes were expressed in an E. coli strain harboring a heterologous mevalonate pathway designed to enhance terpene production, and their product profiles were determined using Solid Phase Micro-Extraction (SPME) and GC-MS. Lastly, out of the 26 TPS’s profiled, 12 TPS’s were functional, with the majority of them exhibiting both monoterpene and sesquiterpene synthase activity.},
doi = {10.1371/journal.pone.0146983},
journal = {PLoS ONE},
number = 2,
volume = 11,
place = {United States},
year = {Wed Feb 17 00:00:00 EST 2016},
month = {Wed Feb 17 00:00:00 EST 2016}
}

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Works referenced in this record:

Geranyl diphosphate synthase from Abies grandis: cDNA isolation, functional expression, and characterization
journal, September 2002


cDNA isolation, functional expression, and characterization of (+)-α-pinene synthase and (−)-α-pinene synthase from loblolly pine (Pinus taeda): Stereocontrol in pinene biosynthesis
journal, March 2003

  • Phillips, Michael A.; Wildung, Mark R.; Williams, David C.
  • Archives of Biochemistry and Biophysics, Vol. 411, Issue 2, p. 267-276
  • DOI: 10.1016/S0003-9861(02)00746-4