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Title: Rapid discovery and functional characterization of terpene synthases from four endophytic xylariaceae

Journal Article · · PLoS ONE
 [1];  [1];  [1];  [2];  [2];  [3];  [4]
  1. Sandia National Lab. (SNL-CA), Livermore, CA (United States)
  2. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Joint BioEnergy Institute, Emeryville, CA (United States)
  3. Sandia National Lab. (SNL-CA), Livermore, CA (United States); Joint BioEnergy Institute, Emeryville, CA (United States)
  4. Michigan State Univ., East Lansing, MI (United States)
+ Show Author Affiliations

© 2016 Wu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in anymedium, provided the original author and source are credited. Endophytic fungi are ubiquitous plant endosymbionts that establish complex and poorly understood relationships with their host organisms. Many endophytic fungi are known to produce a wide spectrum of volatile organic compounds (VOCs) with potential energy applications, which have been described as "mycodiesel". Many of these mycodiesel hydrocarbons are terpenes, a chemically diverse class of compounds produced by many plants, fungi, and bacteria. Due to their high energy densities, terpenes, such as pinene and bisabolene, are actively being investigated as potential "drop-in" biofuels for replacing diesel and aviation fuel. In this study, we rapidly discovered and characterized 26 terpene synthases (TPSs) derived from four endophytic fungi known to produce mycodiesel hydrocarbons. The TPS genes were expressed in an E. coli strain harboring a heterologous mevalonate pathway designed to enhance terpene production, and their product profiles were determined using Solid Phase Micro-Extraction (SPME) and GC-MS. Out of the 26 TPS's profiled, 12 TPS's were functional, with the majority of them exhibiting both monoterpene and sesquiterpene synthase activity.

Research Organization:
Sandia National Lab. (SNL-CA), Livermore, CA (United States); Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE National Nuclear Security Administration (NNSA); USDOE Office of Science (SC), Biological and Environmental Research (BER)
Grant/Contract Number:
AC04-94AL85000; AC02-05CH11231
OSTI ID:
1257274
Alternate ID(s):
OSTI ID: 1266786; OSTI ID: 1333928; OSTI ID: 1379058
Report Number(s):
SAND-2015-5056J
Journal Information:
PLoS ONE, Vol. 11, Issue 2; ISSN 1932-6203
Publisher:
Public Library of ScienceCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 22 works
Citation information provided by
Web of Science

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Cited By (5)

Characterization of four endophytic fungi as potential consolidated bioprocessing hosts for conversion of lignocellulose into advanced biofuels journal January 2017
Engineering Escherichia coli for the production of terpene mixture enriched in caryophyllene and caryophyllene alcohol as potential aviation fuel compounds journal June 2018
Sesquiterpenes in grapes and wines: Occurrence, biosynthesis, functionality, and influence of winemaking processes journal December 2019
Diversity of biologically active secondary metabolites from endophytic and saprotrophic fungi of the ascomycete order Xylariales journal January 2018
Foliar endophytic fungi: diversity in species and functions in forest ecosystems journal January 2020

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
terpenes
sequence motif analysis
fungi
fungal genomics
biosynthesis
cations
cluster compounds
sequence alignment
sequence ailment