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Title: High-quality permanent draft genome sequence of Ensifer meliloti strain 4H41, an effective salt- and drought-tolerant microsymbiont of Phaseolus vulgaris

Abstract

We report that Ensifer meliloti 4H41 is an aerobic, motile, Gram-negative, non-spore-forming rod that can exist as a soil saprophyte or as a legume microsymbiont of common bean (Phaseolus vulgaris). Strain 4H41 was isolated in 2002 from root nodules of P. vulgaris grown in South Tunisia from the oasis of Rjim-Maatoug. Strain 4H41 is salt- and drought-tolerant and highly effective at fixing nitrogen with P. vulgaris. Here we describe the features of E. meliloti 4H41, together with genome sequence information and its annotation. The 6,795,637 bp high-quality permanent draft genome is arranged into 47 scaffolds of 47 contigs containing 6,350 protein-coding genes and 72 RNA-only encoding genes, and is one of the rhizobial genomes sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project proposal.

Authors:
 [1];  [2];  [2];  [3];  [3];  [3];  [3];  [4];  [3];  [5];  [2]
  1. Centre of Biotechnology of Borj-Cedria, Hammam-Lif (Tunisia)
  2. Murdoch Univ., Western Australia (Australia)
  3. DOE Joint Genome Inst., Walnut Creek, CA (United States)
  4. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  5. DOE Joint Genome Inst., Walnut Creek, CA (United States); King Abdulaziz Univ., Jeddah (Saudi Arabia)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1256908
Grant/Contract Number:
AC02-05CH11231
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Standards in Genomic Sciences
Additional Journal Information:
Journal Volume: 10; Journal Issue: 1; Journal ID: ISSN 1944-3277
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Root-nodule bacteria; Nitrogen fixation; Alphaproteobacteria; Ensifer; Phaseolus vulgaris

Citation Formats

Mhamdi, Ridha, Ardley, Julie, Tian, Rui, Seshadri, Rekha, Reddy, T. B. K., Pati, Amrita, Woyke, Tanja, Markowitz, Victor, Ivanova, Natalia, Kyrpides, Nikos, and Reeve, Wayne. High-quality permanent draft genome sequence of Ensifer meliloti strain 4H41, an effective salt- and drought-tolerant microsymbiont of Phaseolus vulgaris. United States: N. p., 2015. Web. doi:10.1186/s40793-015-0005-1.
Mhamdi, Ridha, Ardley, Julie, Tian, Rui, Seshadri, Rekha, Reddy, T. B. K., Pati, Amrita, Woyke, Tanja, Markowitz, Victor, Ivanova, Natalia, Kyrpides, Nikos, & Reeve, Wayne. High-quality permanent draft genome sequence of Ensifer meliloti strain 4H41, an effective salt- and drought-tolerant microsymbiont of Phaseolus vulgaris. United States. doi:10.1186/s40793-015-0005-1.
Mhamdi, Ridha, Ardley, Julie, Tian, Rui, Seshadri, Rekha, Reddy, T. B. K., Pati, Amrita, Woyke, Tanja, Markowitz, Victor, Ivanova, Natalia, Kyrpides, Nikos, and Reeve, Wayne. 2015. "High-quality permanent draft genome sequence of Ensifer meliloti strain 4H41, an effective salt- and drought-tolerant microsymbiont of Phaseolus vulgaris". United States. doi:10.1186/s40793-015-0005-1. https://www.osti.gov/servlets/purl/1256908.
@article{osti_1256908,
title = {High-quality permanent draft genome sequence of Ensifer meliloti strain 4H41, an effective salt- and drought-tolerant microsymbiont of Phaseolus vulgaris},
author = {Mhamdi, Ridha and Ardley, Julie and Tian, Rui and Seshadri, Rekha and Reddy, T. B. K. and Pati, Amrita and Woyke, Tanja and Markowitz, Victor and Ivanova, Natalia and Kyrpides, Nikos and Reeve, Wayne},
abstractNote = {We report that Ensifer meliloti 4H41 is an aerobic, motile, Gram-negative, non-spore-forming rod that can exist as a soil saprophyte or as a legume microsymbiont of common bean (Phaseolus vulgaris). Strain 4H41 was isolated in 2002 from root nodules of P. vulgaris grown in South Tunisia from the oasis of Rjim-Maatoug. Strain 4H41 is salt- and drought-tolerant and highly effective at fixing nitrogen with P. vulgaris. Here we describe the features of E. meliloti 4H41, together with genome sequence information and its annotation. The 6,795,637 bp high-quality permanent draft genome is arranged into 47 scaffolds of 47 contigs containing 6,350 protein-coding genes and 72 RNA-only encoding genes, and is one of the rhizobial genomes sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project proposal.},
doi = {10.1186/s40793-015-0005-1},
journal = {Standards in Genomic Sciences},
number = 1,
volume = 10,
place = {United States},
year = 2015,
month = 7
}

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