Scar-less multi-part DNA assembly design automation
The present invention provides a method of a method of designing an implementation of a DNA assembly. In an exemplary embodiment, the method includes (1) receiving a list of DNA sequence fragments to be assembled together and an order in which to assemble the DNA sequence fragments, (2) designing DNA oligonucleotides (oligos) for each of the DNA sequence fragments, and (3) creating a plan for adding flanking homology sequences to each of the DNA oligos. In an exemplary embodiment, the method includes (1) receiving a list of DNA sequence fragments to be assembled together and an order in which to assemble the DNA sequence fragments, (2) designing DNA oligonucleotides (oligos) for each of the DNA sequence fragments, and (3) creating a plan for adding optimized overhang sequences to each of the DNA oligos.
- Research Organization:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC02-05CH11231
- Assignee:
- The Regents of the University Of California
- Patent Number(s):
- 9,361,427
- Application Number:
- 13/364,285
- OSTI ID:
- 1255960
- Resource Relation:
- Patent File Date: 2012 Feb 01
- Country of Publication:
- United States
- Language:
- English
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Scar-less multi-part DNA assembly design automation
A Fast Scalable, Automated, One-Pot Process for Assembly of DNA Oligonucleotides into Large Gene Sequences