skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Investigation of genetic divergence and polymorphism of nuclear DNA in species and populations of domestic and wild sheep

Abstract

Genetic divergence in repetitive sequences of nuclear DNA of wild and domestic sheep was studied by general restriction endonuclease mapping (i.e., the taxonoprint method). The PCR RAPD method with one and two arbitrary primers was also used to analyze the nuclear DNA polymorphism in some other regions. The taxonoprint method, performed using six endonucleases, showed specificity and virtually complete similarity in the patterns of repetitive DNA sequences of two wild forms, argali and moufflon, and five domestic sheep breeds. Central Asian breeds, Kazakh fine-fleeced, karakuk, ghissar, and eadeelbay, and an English breed, Lincoln, were examined. The results confirm the opinion that wild and domestic sheep may be considered one polytypic species. The PCR-RAPD method, both with one and two arbitrary primers, revealed a closer similarity of all the sheep breeds examined when aragali, rather than with moufflon, was used. These results indicate that the domestication area of sheep was much more broader than was earlier presumed. Otherwise, hybridizations of domestic and wild forms could occasionally occur in the area of their coexistence. The amplification patterns of PCR-RAPD products are the most promising population genetic markers. 27 refs., 4 figs., 7 tabs.

Authors:
;  [1];  [2]
  1. Moscow State Univ. (Russian Federation)
  2. Engelhardt Institute of Molecular Biology, Moscow (Russian Federation)
Publication Date:
OSTI Identifier:
125452
Resource Type:
Journal Article
Journal Name:
Russian Journal of Genetics
Additional Journal Information:
Journal Volume: 31; Journal Issue: 8; Other Information: PBD: Aug 1995; TN: Translated from Genetika; 31: No. 8, 1120-1131(1995)
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; SHEEP; HYBRIDIZATION; POPULATION DYNAMICS; DNA; GENETIC VARIABILITY; DNA SEQUENCING; OLIGONUCLEOTIDES; SIZE; GENETIC MAPPING; POLYMERASE CHAIN REACTION; BIOLOGICAL MARKERS

Citation Formats

Mel`nikova, M N, Grechko, V V, and Mednikov, B M. Investigation of genetic divergence and polymorphism of nuclear DNA in species and populations of domestic and wild sheep. United States: N. p., 1995. Web.
Mel`nikova, M N, Grechko, V V, & Mednikov, B M. Investigation of genetic divergence and polymorphism of nuclear DNA in species and populations of domestic and wild sheep. United States.
Mel`nikova, M N, Grechko, V V, and Mednikov, B M. 1995. "Investigation of genetic divergence and polymorphism of nuclear DNA in species and populations of domestic and wild sheep". United States.
@article{osti_125452,
title = {Investigation of genetic divergence and polymorphism of nuclear DNA in species and populations of domestic and wild sheep},
author = {Mel`nikova, M N and Grechko, V V and Mednikov, B M},
abstractNote = {Genetic divergence in repetitive sequences of nuclear DNA of wild and domestic sheep was studied by general restriction endonuclease mapping (i.e., the taxonoprint method). The PCR RAPD method with one and two arbitrary primers was also used to analyze the nuclear DNA polymorphism in some other regions. The taxonoprint method, performed using six endonucleases, showed specificity and virtually complete similarity in the patterns of repetitive DNA sequences of two wild forms, argali and moufflon, and five domestic sheep breeds. Central Asian breeds, Kazakh fine-fleeced, karakuk, ghissar, and eadeelbay, and an English breed, Lincoln, were examined. The results confirm the opinion that wild and domestic sheep may be considered one polytypic species. The PCR-RAPD method, both with one and two arbitrary primers, revealed a closer similarity of all the sheep breeds examined when aragali, rather than with moufflon, was used. These results indicate that the domestication area of sheep was much more broader than was earlier presumed. Otherwise, hybridizations of domestic and wild forms could occasionally occur in the area of their coexistence. The amplification patterns of PCR-RAPD products are the most promising population genetic markers. 27 refs., 4 figs., 7 tabs.},
doi = {},
url = {https://www.osti.gov/biblio/125452}, journal = {Russian Journal of Genetics},
number = 8,
volume = 31,
place = {United States},
year = {Tue Aug 01 00:00:00 EDT 1995},
month = {Tue Aug 01 00:00:00 EDT 1995}
}