skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Simultaneous Production of Triacylglycerol and High-Value Carotenoids by the Astaxanthin-Producing Oleaginous Green Microalga Chlorella zofingiensis

Abstract

The production of lipids and astaxanthin, a high-value carotenoid, by Chlorella zofingiensis was investigated under different culture conditions. Comparative analysis revealed a good correlation between triacylglycerol (TAG) and astaxanthin accumulation in C. zofingiensis. Stress conditions promoted cell size and weight and induced the accumulation of neutral lipids, especially TAG and astaxanthin, with a concomitant decrease in membrane lipids. The highest contents of TAG and astaxanthin achieved were 387 and 4.89 mg g-1 dry weight, respectively. A semi-continuous culture strategy was developed to optimize the TAG and astaxanthin productivities, which reached 297 and 3.3 mg L-1 day-1, respectively. Additionally, astaxanthin accumulation was enhanced by inhibiting de novo fatty acid biosynthesis. In summary, our study represents a pioneering work of utilizing Chlorella for the integrated production of lipids and high-value products and C. zofingiensis has great potential to be a promising production strain and serve as an emerging oleaginous model alga.

Authors:
; ; ;
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE)
OSTI Identifier:
1254111
Report Number(s):
NREL/JA-5100-66476
Journal ID: ISSN 0960-8524
DOE Contract Number:  
AC36-08GO28308
Resource Type:
Journal Article
Resource Relation:
Journal Name: Bioresource Technology; Journal Volume: 214; Related Information: Bioresource Technology
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; astaxanthin; biofuels; Chlorella zofingiensis; integrated production; stress conditions; triacylglycerol

Citation Formats

Liu, Jin, Mao, Xuemei, Zhou, Wenguang, and Guarnieri, Michael T. Simultaneous Production of Triacylglycerol and High-Value Carotenoids by the Astaxanthin-Producing Oleaginous Green Microalga Chlorella zofingiensis. United States: N. p., 2016. Web. doi:10.1016/j.biortech.2016.04.112.
Liu, Jin, Mao, Xuemei, Zhou, Wenguang, & Guarnieri, Michael T. Simultaneous Production of Triacylglycerol and High-Value Carotenoids by the Astaxanthin-Producing Oleaginous Green Microalga Chlorella zofingiensis. United States. doi:10.1016/j.biortech.2016.04.112.
Liu, Jin, Mao, Xuemei, Zhou, Wenguang, and Guarnieri, Michael T. Mon . "Simultaneous Production of Triacylglycerol and High-Value Carotenoids by the Astaxanthin-Producing Oleaginous Green Microalga Chlorella zofingiensis". United States. doi:10.1016/j.biortech.2016.04.112.
@article{osti_1254111,
title = {Simultaneous Production of Triacylglycerol and High-Value Carotenoids by the Astaxanthin-Producing Oleaginous Green Microalga Chlorella zofingiensis},
author = {Liu, Jin and Mao, Xuemei and Zhou, Wenguang and Guarnieri, Michael T.},
abstractNote = {The production of lipids and astaxanthin, a high-value carotenoid, by Chlorella zofingiensis was investigated under different culture conditions. Comparative analysis revealed a good correlation between triacylglycerol (TAG) and astaxanthin accumulation in C. zofingiensis. Stress conditions promoted cell size and weight and induced the accumulation of neutral lipids, especially TAG and astaxanthin, with a concomitant decrease in membrane lipids. The highest contents of TAG and astaxanthin achieved were 387 and 4.89 mg g-1 dry weight, respectively. A semi-continuous culture strategy was developed to optimize the TAG and astaxanthin productivities, which reached 297 and 3.3 mg L-1 day-1, respectively. Additionally, astaxanthin accumulation was enhanced by inhibiting de novo fatty acid biosynthesis. In summary, our study represents a pioneering work of utilizing Chlorella for the integrated production of lipids and high-value products and C. zofingiensis has great potential to be a promising production strain and serve as an emerging oleaginous model alga.},
doi = {10.1016/j.biortech.2016.04.112},
journal = {Bioresource Technology},
number = ,
volume = 214,
place = {United States},
year = {Mon Aug 01 00:00:00 EDT 2016},
month = {Mon Aug 01 00:00:00 EDT 2016}
}