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Title: Direct analysis of complex mixtures by mass spectrometry

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USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
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Journal Article: Publisher's Accepted Manuscript
Journal Name:
International Journal of Mass Spectrometry
Additional Journal Information:
Journal Volume: 377; Journal Issue: C; Related Information: CHORUS Timestamp: 2017-05-18 03:06:39; Journal ID: ISSN 1387-3806
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Citation Formats

Cooks, R. Graham, Jarmusch, Alan K., and Wleklinski, Michael. Direct analysis of complex mixtures by mass spectrometry. Netherlands: N. p., 2015. Web. doi:10.1016/j.ijms.2014.06.035.
Cooks, R. Graham, Jarmusch, Alan K., & Wleklinski, Michael. Direct analysis of complex mixtures by mass spectrometry. Netherlands. doi:10.1016/j.ijms.2014.06.035.
Cooks, R. Graham, Jarmusch, Alan K., and Wleklinski, Michael. 2015. "Direct analysis of complex mixtures by mass spectrometry". Netherlands. doi:10.1016/j.ijms.2014.06.035.
title = {Direct analysis of complex mixtures by mass spectrometry},
author = {Cooks, R. Graham and Jarmusch, Alan K. and Wleklinski, Michael},
abstractNote = {},
doi = {10.1016/j.ijms.2014.06.035},
journal = {International Journal of Mass Spectrometry},
number = C,
volume = 377,
place = {Netherlands},
year = 2015,
month = 2

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at 10.1016/j.ijms.2014.06.035

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Cited by: 5works
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  • State-of-the-art proteomics workflows involve multiple interdependent steps: sample preparation, protein peptide separation, mass spectrometry and data analysis.While improvements in any of these steps can increase the depth and breadth of analysis, advances in mass spectrometry have catalysed many of the most important developments. We discuss common classes of mass analysers and how these analysers are put together to produce some of the most popular mass spectrometry platforms.The capabilities of these platforms determine how they can be used in a variety of common proteomic strategies and, in turn, what types of biological questions can be addressed. Moving forward, powerful new hybridmassmore » spectrometers and application of emerging types of tandemmass spectrometry promise that our ability to analyse complex mixtures of proteins will continue to advance.« less
  • Reactive nanospray desorption electrospray ionization (nano-DESI) combined with high-resolution mass spectrometry was utilized for the analysis of secondary organic aerosol produced through ozonolysis of limonene (LSOA). Previous studies showed that LSOA constituents are multifunctional compounds containing aldehyde and ketone groups. In this study, we used the selectivity of the Girard T (GT) reagent towards carbonyl compounds to examine the utility of reactive nano-DESI for the analysis of complex organic mixtures. In these experiments, 1-100 {micro}M GT solution was used as a working solvent for reactive nano-DESI analysis. Abundant products of a single addition of GT to LSOA constituents were observedmore » at GT concentrations in excess of 10 {micro}M. We found that LSOA compounds with 18-20 carbon atoms (dimers) and 27-30 carbon atoms (trimers) react with GT through a simple addition reaction resulting in formation of the carbinolamine derivative. In contrast, reactions of GT with monomeric species result in formation of both the carbinolamine and the hydrazone derivatives. In addition, several monomers did not react with GT on the timescale of our experiment. These molecules were characterized by relatively high values of the double bond equivalent (DBE) and low oxygen content. Furthermore, because addition of a charged GT tag to a neutral molecule eliminates the discrimination against the low proton affinity compounds in the ionization process, reactive nano-DESI analysis enables quantification of individual compounds in the complex mixture. For example, we were able to estimate for the first time the amounts of dimers and trimers in the LSOA mixture. Specifically, we found that the most abundant LSOA dimer was detected at ca. 0.5 pg level and the total amount of dimers and trimers in the analyzed sample was just around 11 pg. Our results indicate that reactive nano-DESI is a valuable approach for examining the presence of specific functional groups and quantification of compounds possessing these groups in complex mixtures.« less