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Title: Fully integrated wearable sensor arrays for multiplexed in situ perspiration analysis

Abstract

© 2016 Macmillan Publishers Limited. All rights reserved. Wearable sensor technologies are essential to the realization of personalized medicine through continuously monitoring an individual's state of health. Sampling human sweat, which is rich in physiological information, could enable non-invasive monitoring. Previously reported sweat-based and other non-invasive biosensors either can only monitor a single analyte at a time or lack on-site signal processing circuitry and sensor calibration mechanisms for accurate analysis of the physiological state. Given the complexity of sweat secretion, simultaneous and multiplexed screening of target biomarkers is critical and requires full system integration to ensure the accuracy of measurements. Here we present a mechanically flexible and fully integrated (that is, no external analysis is needed) sensor array for multiplexed in situ perspiration analysis, which simultaneously and selectively measures sweat metabolites (such as glucose and lactate) and electrolytes (such as sodium and potassium ions), as well as the skin temperature (to calibrate the response of the sensors). Our work bridges the technological gap between signal transduction, conditioning (amplifi cation and filtering), processing and wireless transmission in wearable biosensors by merging plastic-based sensors that interface with the skin with silicon integrated circuits consolidated on a flexible circuit board for complex signalmore » processing. This application could not have been realized using either of these technologies alone owing to their respective inherent limitations. The wearable system is used to measure the detailed sweat profile of human subjects engaged in prolonged indoor and outdoor physical activities, and to make a real-time assessment of the physiological state of the subjects. This platform enables a wide range of personalized diagnostic and physiological monitoring applications.« less

Authors:
 [1];  [1];  [1];  [2];  [1];  [3];  [1];  [1];  [1];  [1];  [4];  [3];  [2];  [4]
  1. Univ. of California, Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Materials Sciences Division
  2. Stanford Shool of Medicine, Palo Alto, CA (United States). Stanford Genome Center
  3. Univ. of California, Berkeley, CA (United States)
  4. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Materials Sciences Division
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1252977
Alternate Identifier(s):
OSTI ID: 1414743
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Nature (London)
Additional Journal Information:
Journal Volume: 529; Journal Issue: 7587; Journal ID: ISSN 0028-0836
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION; electrical and electronic engineering; materials for devices; techniques and instrumentation; health care

Citation Formats

Gao, Wei, Emaminejad, Sam, Nyein, Hnin Yin Yin, Challa, Samyuktha, Chen, Kevin, Peck, Austin, Fahad, Hossain M., Ota, Hiroki, Shiraki, Hiroshi, Kiriya, Daisuke, Lien, Der-Hsien, Brooks, George A., Davis, Ronald W., and Javey, Ali. Fully integrated wearable sensor arrays for multiplexed in situ perspiration analysis. United States: N. p., 2016. Web. doi:10.1038/nature16521.
Gao, Wei, Emaminejad, Sam, Nyein, Hnin Yin Yin, Challa, Samyuktha, Chen, Kevin, Peck, Austin, Fahad, Hossain M., Ota, Hiroki, Shiraki, Hiroshi, Kiriya, Daisuke, Lien, Der-Hsien, Brooks, George A., Davis, Ronald W., & Javey, Ali. Fully integrated wearable sensor arrays for multiplexed in situ perspiration analysis. United States. https://doi.org/10.1038/nature16521
Gao, Wei, Emaminejad, Sam, Nyein, Hnin Yin Yin, Challa, Samyuktha, Chen, Kevin, Peck, Austin, Fahad, Hossain M., Ota, Hiroki, Shiraki, Hiroshi, Kiriya, Daisuke, Lien, Der-Hsien, Brooks, George A., Davis, Ronald W., and Javey, Ali. Wed . "Fully integrated wearable sensor arrays for multiplexed in situ perspiration analysis". United States. https://doi.org/10.1038/nature16521. https://www.osti.gov/servlets/purl/1252977.
@article{osti_1252977,
title = {Fully integrated wearable sensor arrays for multiplexed in situ perspiration analysis},
author = {Gao, Wei and Emaminejad, Sam and Nyein, Hnin Yin Yin and Challa, Samyuktha and Chen, Kevin and Peck, Austin and Fahad, Hossain M. and Ota, Hiroki and Shiraki, Hiroshi and Kiriya, Daisuke and Lien, Der-Hsien and Brooks, George A. and Davis, Ronald W. and Javey, Ali},
abstractNote = {© 2016 Macmillan Publishers Limited. All rights reserved. Wearable sensor technologies are essential to the realization of personalized medicine through continuously monitoring an individual's state of health. Sampling human sweat, which is rich in physiological information, could enable non-invasive monitoring. Previously reported sweat-based and other non-invasive biosensors either can only monitor a single analyte at a time or lack on-site signal processing circuitry and sensor calibration mechanisms for accurate analysis of the physiological state. Given the complexity of sweat secretion, simultaneous and multiplexed screening of target biomarkers is critical and requires full system integration to ensure the accuracy of measurements. Here we present a mechanically flexible and fully integrated (that is, no external analysis is needed) sensor array for multiplexed in situ perspiration analysis, which simultaneously and selectively measures sweat metabolites (such as glucose and lactate) and electrolytes (such as sodium and potassium ions), as well as the skin temperature (to calibrate the response of the sensors). Our work bridges the technological gap between signal transduction, conditioning (amplifi cation and filtering), processing and wireless transmission in wearable biosensors by merging plastic-based sensors that interface with the skin with silicon integrated circuits consolidated on a flexible circuit board for complex signal processing. This application could not have been realized using either of these technologies alone owing to their respective inherent limitations. The wearable system is used to measure the detailed sweat profile of human subjects engaged in prolonged indoor and outdoor physical activities, and to make a real-time assessment of the physiological state of the subjects. This platform enables a wide range of personalized diagnostic and physiological monitoring applications.},
doi = {10.1038/nature16521},
url = {https://www.osti.gov/biblio/1252977}, journal = {Nature (London)},
issn = {0028-0836},
number = 7587,
volume = 529,
place = {United States},
year = {2016},
month = {1}
}

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Works referenced in this record:

User-interactive electronic skin for instantaneous pressure visualization
journal, July 2013


Ultrathin conformal devices for precise and continuous thermal characterization of human skin
journal, September 2013


Highly ordered nanowire arrays on plastic substrates for ultrasensitive flexible chemical sensors
journal, April 2007


In vivo life sign application of dielectric spectroscopy and non-invasive glucose monitoring
journal, December 2007


Flexible and Stretchable Electronics for Biointegrated Devices
journal, August 2012


A reference electrode based on polyvinyl butyral (PVB) polymer for decentralized chemical measurements
journal, April 2014


Soft Microfluidic Assemblies of Sensors, Circuits, and Radios for the Skin
journal, April 2014


An ultra-lightweight design for imperceptible plastic electronics
journal, July 2013


Skin-like pressure and strain sensors based on transparent elastic films of carbon nanotubes
journal, October 2011


A wearable electrochemical sensor for the real-time measurement of sweat sodium concentration
journal, January 2010


Piezoelectricity of single-atomic-layer MoS2 for energy conversion and piezotronics
journal, October 2014


Epidermal Electronics
journal, August 2011


Non-invasive wearable electrochemical sensors: a review
journal, July 2014


Thin-Film Microbiosensors for Glucose−Lactate Monitoring
journal, January 1996


The relationship between exercise intensity and the sweat lactate excretion rate
journal, December 2009


Clinical Skin Temperature Measurement to Predict Incipient Pressure Ulcers
journal, January 2001


Lactate in human sweat: a critical review of research to the present day
journal, June 2012


Lactate kinetics at the lactate threshold in trained and untrained men
journal, June 2013


Acute effects of dehydration on sweat composition in men during prolonged exercise in the heat
journal, September 2004


Epidermal tattoo potentiometric sodium sensors with wireless signal transduction for continuous non-invasive sweat monitoring
journal, April 2014


Tattoo-Based Noninvasive Glucose Monitoring: A Proof-of-Concept Study
journal, December 2014


Nanowire active-matrix circuitry for low-voltage macroscale artificial skin
journal, September 2010


BIOTEX—Biosensing Textiles for Personalised Healthcare Management
journal, March 2010


Exercise-associated hyponatremia: A review
journal, March 2001


Comparison of sweat rate during graded exercise and the local rate induced by pilocarpine
journal, July 2005


Variations in Regional Sweat Composition in Normal Human Males
journal, November 2000


Preparation of all solid-state potentiometric ion sensors with polymer-CNT composites
journal, August 2009


A flexible and wearable glucose sensor based on functional polymers with Soft-MEMS techniques
journal, October 2006


Adhesive RFID Sensor Patch for Monitoring of Sweat Electrolytes
journal, June 2015


Effects of Dehydration on Exercise Performance
journal, April 1999


Elastomeric polymer light-emitting devices and displays
journal, September 2013


A review of wearable sensors and systems with application in rehabilitation
journal, January 2012


Electrochemical Tattoo Biosensors for Real-Time Noninvasive Lactate Monitoring in Human Perspiration
journal, July 2013


Electrochemical Biosensors - Sensor Principles and Architectures
journal, March 2008


A modified anaerobic method of sweat collection
journal, May 1984


Importance of skin temperature in the regulation of sweating.
journal, July 1971


Effects of water intake on composition of thermal sweat in normal human volunteers
journal, November 1970


Electrochemical Biosensors - Sensor Principles and Architectures
journal, March 2008


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    A skin-like stretchable colorimetric temperature sensor
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    Direct electrochemical biosensing in gastrointestinal fluids
    journal, December 2018


    High‐Transconductance Stretchable Transistors Achieved by Controlled Gold Microcrack Morphology
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    An Overview of the Development of Flexible Sensors
    journal, July 2017


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    Planar‐Type Printed Flexible Mechanical Switch
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    A mass-customizable dermal patch with discrete colorimetric indicators for personalized sweat rate quantification
    journal, June 2019


    Palladium/palladium oxide coated electrospun fibers for wearable sweat pH-sensors
    journal, June 2019


    Wearable sensors for monitoring the physiological and biochemical profile of the athlete
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    Developing a new device for continuously recording, in vivo, the excretion rate of sweat (perspiration) in humans
    journal, January 2019


    A Combinatorial Electrochemical Biosensor for Sweat Biomarker Benchmarking
    journal, October 2019


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    journal, November 2019


    Assessment of the Fitbit Charge 2 for monitoring heart rate
    journal, February 2018


    Epidermal Inorganic Optoelectronics for Blood Oxygen Measurement
    journal, February 2017


    An Overview of the Development of Flexible Sensors
    journal, July 2017


    Flexible 1D Batteries: Recent Progress and Prospects
    journal, July 2019


    Flexible Hybrid Electronics for Digital Healthcare
    journal, June 2019


    Modular and Reconfigurable Wireless E‐Tattoos for Personalized Sensing
    journal, May 2019


    Toward Flexible Surface‐Enhanced Raman Scattering (SERS) Sensors for Point‐of‐Care Diagnostics
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    Planar‐Type Printed Flexible Mechanical Switch
    journal, June 2018


    High‐Transconductance Stretchable Transistors Achieved by Controlled Gold Microcrack Morphology
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    Direct electrochemical biosensing in gastrointestinal fluids
    journal, December 2018


    User-centered Interface Design Approach for a Smart Heated Garment
    journal, January 2018


    A skin-like stretchable colorimetric temperature sensor
    journal, April 2018


    A mass-customizable dermal patch with discrete colorimetric indicators for personalized sweat rate quantification
    journal, June 2019


    Palladium/palladium oxide coated electrospun fibers for wearable sweat pH-sensors
    journal, June 2019


    Wearable sensors for monitoring the physiological and biochemical profile of the athlete
    journal, July 2019


    A flexible, ultra-sensitive chemical sensor with 3D biomimetic templating for diabetes-related acetone detection
    journal, January 2017


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    journal, November 2019


    Developing a new device for continuously recording, in vivo, the excretion rate of sweat (perspiration) in humans
    journal, January 2019


    Thermomechanical Analysis of Epidermal Electronic Devices Integrated With Human Skin
    journal, September 2017


    A Combinatorial Electrochemical Biosensor for Sweat Biomarker Benchmarking
    journal, October 2019


    Simple Fabrication of Flexible Biosensor Arrays Using Direct Writing for Multianalyte Measurement from Human Astrocytes
    journal, November 2019


    CdSSe nanowire-chip based wearable sweat sensor
    journal, March 2019


    Assessment of the Fitbit Charge 2 for monitoring heart rate
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    Stretchable bioelectronics—Current and future
    journal, December 2017


    Wearable Woven Triboelectric Nanogenerator Utilizing Electrospun PVDF Nanofibers for Mechanical Energy Harvesting
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    Size-Scalable and High-Density Liquid-Metal-Based Soft Electronic Passive Components and Circuits Using Soft Lithography
    journal, November 2016


    Restickable Oxide Neuromorphic Transistors with Spike-Timing-Dependent Plasticity and Pavlovian Associative Learning Activities
    journal, September 2018


    Flexible Crossbar‐Structured Phase Change Memory Array via Mo‐Based Interfacial Physical Lift‐Off
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    Microparticle‐Based Soft Electronic Devices: Toward One‐Particle/One‐Pixel
    journal, May 2019


    Wearable Wireless Tyrosinase Bandage and Microneedle Sensors: Toward Melanoma Screening
    journal, January 2018


    Mechanically Reinforced Skin-Electronics with Networked Nanocomposite Elastomer
    journal, October 2016


    Full Dynamic-Range Pressure Sensor Matrix Based on Optical and Electrical Dual-Mode Sensing
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    Materials and Wearable Devices for Autonomous Monitoring of Physiological Markers
    journal, March 2018


    Vitrimer Elastomer-Based Jigsaw Puzzle-Like Healable Triboelectric Nanogenerator for Self-Powered Wearable Electronics
    journal, February 2018


    A Colloidal‐Quantum‐Dot‐Based Self‐Charging System via the Near‐Infrared Band
    journal, May 2018


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    journal, April 2018


    3D Printed Functional and Biological Materials on Moving Freeform Surfaces
    journal, April 2018


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    journal, July 2018


    Disposable Sensors in Diagnostics, Food, and Environmental Monitoring
    journal, May 2019


    Organic Photodetectors for Next‐Generation Wearable Electronics
    journal, August 2019


    Material‐Based Approaches for the Fabrication of Stretchable Electronics
    journal, April 2020


    A Flexible Carbon Nanotube Sen‐Memory Device
    journal, January 2020


    Flexible Difunctional (Pressure and Light) Sensors Based on ZnO Nanowires/Graphene Heterostructures
    journal, January 2020


    Portable Multiplex Optical Assays
    journal, December 2018


    Robotics in the Gut
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    Simultaneous Monitoring of Sweat and Interstitial Fluid Using a Single Wearable Biosensor Platform
    journal, August 2018


    Design and Assembly of Reconfigurable 3D Radio‐Frequency Antennas Based on Mechanically Triggered Switches
    journal, May 2019


    Use of Polymer Coatings to Enhance the Response of Redox‐Polymer‐Mediated Electrodes
    journal, September 2018


    Printed Organic Transistor‐Based Enzyme Sensor for Continuous Glucose Monitoring in Wearable Healthcare Applications
    journal, October 2018


    Wearable Platform for Real-time Monitoring of Sodium in Sweat
    journal, April 2018


    Ionic Gels and Their Applications in Stretchable Electronics
    journal, July 2018


    Large-Scale Direct-Writing of Aligned Nanofibers for Flexible Electronics
    journal, February 2018


    Noninvasive Transdermal Delivery System of Lidocaine Using an Acoustic Droplet-Vaporization Based Wearable Patch
    journal, October 2018


    A MXene‐Based Wearable Biosensor System for High‐Performance In Vitro Perspiration Analysis
    journal, April 2019


    Exercise intensity effects on total sweat electrolyte losses and regional vs. whole-body sweat [Na+], [Cl−], and [K+]
    journal, December 2018


    Carbon Nanofiber-Based Wearable Patches for Bio-Potential Monitoring
    journal, April 2019


    Technological leap for sweat sensing
    journal, January 2016


    Fully integrated wearable impedance cytometry platform on flexible circuit board with online smartphone readout
    journal, July 2018


    Self-powered smart patch for sweat conductivity monitoring
    journal, January 2019


    Monitoring biomolecule concentrations in tissue using a wearable droplet microfluidic-based sensor
    journal, June 2019


    Highly selective detection of methanol over ethanol by a handheld gas sensor
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    journal, September 2019


    Toward a new generation of smart skins
    journal, April 2019


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    Human sweat monitoring using polymer-based fiber
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    Stretchable lithium-air batteries for wearable electronics
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    Top-down fabrication meets bottom-up synthesis for nanoelectronic barcoding of microparticles
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    Flexible quasi-solid-state planar micro-supercapacitor based on cellular graphene films
    journal, January 2017


    A wearable and highly sensitive strain sensor based on a polyethylenimine–rGO layered nanocomposite thin film
    journal, January 2017


    Integrated paper-based microfluidic devices for point-of-care testing
    journal, January 2018


    A general strategy for printing colloidal nanomaterials into one-dimensional micro/nanolines
    journal, January 2018


    Motion recognition by a liquid filled tubular triboelectric nanogenerator
    journal, January 2019


    Three-dimensional helical inorganic thermoelectric generators and photodetectors for stretchable and wearable electronic devices
    journal, January 2018


    An efficient PEDOT-coated textile for wearable thermoelectric generators and strain sensors
    journal, January 2019


    Flexible, low volume detection of chronobiology biomarkers from human sweat
    journal, January 2020


    A wearable electrofluidic actuation system
    journal, January 2019


    Construction of a flexible electrochemiluminescence platform for sweat detection
    journal, January 2019


    Multiscale porous elastomer substrates for multifunctional on-skin electronics with passive-cooling capabilities
    journal, December 2019


    Progress on wearable triboelectric nanogenerators in shapes of fiber, yarn, and textile
    journal, August 2019


    High sensitivity knitted fabric bi-directional pressure sensor based on conductive blended yarn
    journal, February 2019


    Wearable nanosensor systems and their applications in healthcare
    conference, April 2017

    • Ramasamy, Mouli; Kumar, Prashanth S.; Varadan, Vijay K.
    • SPIE Smart Structures and Materials + Nondestructive Evaluation and Health Monitoring, SPIE Proceedings
    • https://doi.org/10.1117/12.2264812

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    A soft, wearable microfluidic device for the capture, storage, and colorimetric sensing of sweat
    journal, November 2016


    Battery-free, wireless sensors for full-body pressure and temperature mapping
    journal, April 2018


    Paper-Based Disk-Type Self-Powered Glucose Biosensor Based on Screen-Printed Biofuel Cell Array
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