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Title: Multiplexed microfluidic approach for nucleic acid enrichment

Abstract

A system for enhancing a nucleic acid sample may include a one pump, a denaturing chamber; a microfluidic hydroxyapatite chromatography device configured for performing hydroxyapatite chromatography on the nucleic acid sample, a sample collector, and tubing connecting the pump with the denaturing chamber, the hydroxyapatite chromatography device and the sample collector such that the pump may be used to move the nucleic acid sample from the denaturing chamber to the hydroxyapatite chromatography device and then to the sample collector.

Inventors:
; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1248895
Patent Number(s):
9,322,014
Application Number:
14/084,937
Assignee:
Sandia Corporation (Albuquerque, NM) SNL-A
DOE Contract Number:  
AC04-94AL85000
Resource Type:
Patent
Resource Relation:
Patent File Date: 2013 Nov 20
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

VanderNoot, Victoria A., Langevin, Stanley Alan, Bent, Zachary, Renzi, Ronald F., Ferko, Scott M., Van De Vreugde, James L., Lane, Todd, Patel, Kamlesh, and Branda, Steven. Multiplexed microfluidic approach for nucleic acid enrichment. United States: N. p., 2016. Web.
VanderNoot, Victoria A., Langevin, Stanley Alan, Bent, Zachary, Renzi, Ronald F., Ferko, Scott M., Van De Vreugde, James L., Lane, Todd, Patel, Kamlesh, & Branda, Steven. Multiplexed microfluidic approach for nucleic acid enrichment. United States.
VanderNoot, Victoria A., Langevin, Stanley Alan, Bent, Zachary, Renzi, Ronald F., Ferko, Scott M., Van De Vreugde, James L., Lane, Todd, Patel, Kamlesh, and Branda, Steven. Tue . "Multiplexed microfluidic approach for nucleic acid enrichment". United States. doi:. https://www.osti.gov/servlets/purl/1248895.
@article{osti_1248895,
title = {Multiplexed microfluidic approach for nucleic acid enrichment},
author = {VanderNoot, Victoria A. and Langevin, Stanley Alan and Bent, Zachary and Renzi, Ronald F. and Ferko, Scott M. and Van De Vreugde, James L. and Lane, Todd and Patel, Kamlesh and Branda, Steven},
abstractNote = {A system for enhancing a nucleic acid sample may include a one pump, a denaturing chamber; a microfluidic hydroxyapatite chromatography device configured for performing hydroxyapatite chromatography on the nucleic acid sample, a sample collector, and tubing connecting the pump with the denaturing chamber, the hydroxyapatite chromatography device and the sample collector such that the pump may be used to move the nucleic acid sample from the denaturing chamber to the hydroxyapatite chromatography device and then to the sample collector.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Apr 26 00:00:00 EDT 2016},
month = {Tue Apr 26 00:00:00 EDT 2016}
}

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Works referenced in this record:

Rare target enrichment for ultrasensitive PCR detection using cot–rehybridization and duplex-specific nuclease
journal, February 2012

  • Gijavanekar, Charul; Strych, Ulrich; Fofanov, Yuriy
  • Analytical Biochemistry, Vol. 421, Issue 1, p. 81-85
  • DOI: 10.1016/j.ab.2011.11.010

Enriching pathogen transcripts from infected samples: A capture-based approach to enhanced host–pathogen RNA sequencing
journal, July 2013

  • Bent, Zachary W.; Tran-Gyamfi, Mary B.; Langevin, Stanley A.
  • Analytical Biochemistry, Vol. 438, Issue 1, p. 90-96
  • DOI: 10.1016/j.ab.2013.03.008

On-line monitoring system for chemical warfare agents using automated capillary micellar electrokinetic chromatography
journal, August 2012

  • VanderNoot, Victoria; Ferko, Scott; Van De Vreugde, James
  • Journal of Chromatography A, Vol. 1249, p. 233-240
  • DOI: 10.1016/j.chroma.2012.06.010